Abstract:
:We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expression, and maximal induction was observed only after 20 min of exposure to paraquat. Neither a mutation in soxS nor one in soxR prevented the paraquat-dependent increase in phi(hmp-lacZ) expression, but either mutant allele delayed full expression of phi(hmp-lacZ) activity after paraquat addition. Induction of hmp by paraquat was demonstrated in aerobically grown cultures during exponential growth and the stationary phase, thus revealing two Sox-independent regulatory mechanisms. Induction of hmp by paraquat in the stationary phase was dependent on the global regulator of stationary-phase gene expression, RpoS (sigma S). However, a mutation in rpoS did not prevent an increase in hmp expression by paraquat in exponentially growing cells. Induction of sigma S in the exponential phase by heat shock also induced phi(hmp-lacZ) expression in the presence of paraquat, supporting the role of sigma S in one of the regulatory mechanisms. Mutations in oxyR or rob, known regulators of several stress promoters in E. coli, had no effect on the induction of hmp by paraquat. Other known superoxide-generating agents (plumbagin, menadione, and phenazine methosulfate) were not effective in inducing hmp expression.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Membrillo-Hernández J,Kim SO,Cook GM,Poole RKdoi
10.1128/jb.179.10.3164-3170.1997subject
Has Abstractpub_date
1997-05-01 00:00:00pages
3164-70issue
10eissn
0021-9193issn
1098-5530journal_volume
179pub_type
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/JB.110.1.281-290.1972
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1105-1108.1979
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.00324-13
更新日期:2013-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.775-779.1986
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doi:10.1128/JB.94.1.141-148.1967
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journal_title:Journal of bacteriology
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doi:10.1128/JB.127.1.536-544.1976
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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doi:10.1128/JB.158.1.180-186.1984
更新日期:1984-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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更新日期:1971-01-01 00:00:00
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1975-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1390-1394.1969
更新日期:1969-06-01 00:00:00
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