Characterization of the ccpA gene of Enterococcus faecalis: identification of starvation-inducible proteins regulated by ccpA.

abstract：:Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...

journal_title：Journal of bacteriology

authors： Morty RE,Fülöp V,Andrews NW

更新日期：2002-06-01 00:00:00

abstract：:Bacteria use three distinct systems for iron-sulfur (Fe/S) cluster biogenesis: the ISC, SUF, and NIF machineries. The ISC and SUF systems are widely distributed, and many bacteria possess both of them. In Escherichia coli, ISC is the major and constitutive system, whereas SUF is induced under iron starvation and/or ox...

journal_title：Journal of bacteriology

authors： Albrecht AG,Netz DJ,Miethke M,Pierik AJ,Burghaus O,Peuckert F,Lill R,Marahiel MA

更新日期：2010-03-01 00:00:00

abstract：:Mouse-virulent Salmonella typhimurium strains SR-11 and ATCC 14028-1s express curli fibers, thin aggregative fibers, at ambient temperature on plates as judged by Western blot analysis and electron microscopy. Concomitantly with curli expression, cells develop a rough and dry colony morphology and bind the dye Congo r...

journal_title：Journal of bacteriology

authors： Römling U,Bian Z,Hammar M,Sierralta WD,Normark S

更新日期：1998-02-01 00:00:00

abstract：:The chemical properties of the cell wall of Mycobacterium lepraemurium strain Hawaii were investigated. Five subunits of the cell wall, arabinose mycolate, mycolic acids, tetrapeptide (Ala-Gln-diaminopimelic acid-Ala), disaccharide (N-acetylglucosaminyl-beta-1,4-N-glycolylmuramic acid), and arabinogalactan, were obtai...

journal_title：Journal of bacteriology

authors： Azuma I,Yamamura Y,Tanaka Y,Kosaka K,Mori T

更新日期：1973-01-01 00:00:00

abstract：:Entomopathogenic bacteria of the genus Photorhabdus harbor a type III secretion system. This system was probably acquired prior to the separation of the species within this genus. Furthermore, the core components of the secretion machinery are highly conserved but the predicted effectors differ between Photorhabdus lu...

journal_title：Journal of bacteriology

authors： Brugirard-Ricaud K,Givaudan A,Parkhill J,Boemare N,Kunst F,Zumbihl R,Duchaud E

更新日期：2004-07-01 00:00:00

abstract：:Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...

journal_title：Journal of bacteriology

authors： Saika A,Ushimaru K,Mizuno S,Tsuge T

更新日期：2015-04-01 00:00:00

abstract：:phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from...

journal_title：Journal of bacteriology

authors： Pajunen MI,Kiljunen SJ,Söderholm ME,Skurnik M

更新日期：2001-03-01 00:00:00

abstract：:The gene for staphylococcal enterotoxin type E (entE) was cloned from Staphylococcus aureus into plasmid vector pBR322 and introduced into Escherichia coli. A staphylococcal enterotoxin type E-producing E. coli strain was isolated. The complete nucleotide sequence of the cloned structural entE gene and the N-terminal ...

journal_title：Journal of bacteriology

authors： Couch JL,Soltis MT,Betley MJ

更新日期：1988-07-01 00:00:00

abstract：:Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

journal_title：Journal of bacteriology

authors： Fitz-James PC

更新日期：1971-03-01 00:00:00

abstract：:We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...

journal_title：Journal of bacteriology

authors： Makino K,Kim SK,Shinagawa H,Amemura M,Nakata A

更新日期：1991-04-01 00:00:00

abstract：:The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...

journal_title：Journal of bacteriology

authors： O'Connor M,Gregory ST

更新日期：2011-01-01 00:00:00

abstract：:Gel retardation experiments indicated the presence in Pseudomonas aeruginosa cell extracts of an arginine-inducible DNA-binding protein that interacts with the control regions for the car and argF operons, encoding carbamoylphosphate synthetase and anabolic ornithine carbamoyltransferase, respectively. Both enzymes ar...

journal_title：Journal of bacteriology

authors： Park SM,Lu CD,Abdelal AT

更新日期：1997-09-01 00:00:00

abstract：:The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...

journal_title：Journal of bacteriology

authors： Kung FC,Raymond J,Glaser DA

更新日期：1976-06-01 00:00:00

abstract：:Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

journal_title：Journal of bacteriology

authors： ELLNER PD,BOHAN CD

更新日期：1962-02-01 00:00:00

abstract：:Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

journal_title：Journal of bacteriology

authors： Franzetti B,Sohlberg B,Zaccai G,von Gabain A

更新日期：1997-02-01 00:00:00

abstract：:The ability to reduce Hg(II) to Hg(0), which is determined by a plasmid-borne gene in Escherichia coli, is conferred by a Hg(II)-inducible activity which is located in the cytoplasm rather than in the periplasmic space of the cell. This Hg(II)-reducing activity can be isolated from the supernatant of a 160,000 x g cen...

journal_title：Journal of bacteriology

authors： Summers AO,Sugarman LI

更新日期：1974-07-01 00:00:00

abstract：:The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...

journal_title：Journal of bacteriology

authors： Ying CW,Ordal GW

更新日期：1989-03-01 00:00:00

abstract：:DNA helicase I, encoded on the Escherichia coli F plasmid, catalyzes a site- and strand-specific nicking reaction within the F plasmid origin of transfer (oriT) to initiate conjugative DNA strand transfer. The product of the nicking reaction contains a single phosphodiester bond interruption as determined by single-nu...

journal_title：Journal of bacteriology

authors： Matson SW,Nelson WC,Morton BS

更新日期：1993-05-01 00:00:00

abstract：:The GcvA protein is a LysR family regulatory protein necessary for both activation and repression of the Escherichia coli glycine cleavage enzyme operon (gcv) and negative regulation of gcvA. Gel shift assays indicated that overexpressed GcvA in crude extracts is capable of binding specifically to DNA containing the g...

journal_title：Journal of bacteriology

authors： Wilson RL,Urbanowski ML,Stauffer GV

更新日期：1995-09-01 00:00:00

abstract：:Erwinia chrysanthemi 3937 secretes several pectinolytic enzymes, among which eight isoenzymes of pectate lyases with an endo-cleaving mode (PelA, PelB, PelC, PelD, PelE, PelI, PelL, and PelZ) have been identified. Two exo-cleaving enzymes, the exopolygalacturonate lyase, PelX, and an exo-poly-alpha-D-galacturonosidase...

journal_title：Journal of bacteriology

authors： Shevchik VE,Kester HC,Benen JA,Visser J,Robert-Baudouy J,Hugouvieux-Cotte-Pattat N

更新日期：1999-03-01 00:00:00

abstract：:Temperate bacteriophages effect chromosomal evolution of their bacterial hosts, mediating rearrangements and the acquisition of novel genes from other taxa. Although the Haemophilus influenzae genome shows evidence of past phage-mediated lateral transfer, the phages presumed responsible have not been identified. To da...

journal_title：Journal of bacteriology

authors： Williams BJ,Golomb M,Phillips T,Brownlee J,Olson MV,Smith AL

更新日期：2002-12-01 00:00:00

abstract：:A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...

journal_title：Journal of bacteriology

authors： Elorza MV,Sentandreu R,Ruiz-Herrera J

更新日期：1994-04-01 00:00:00

abstract：:Adsorption of phages T4, T5, and BF23 to previously starved Escherichia coli cells triggered the immediate release of respiratory control. A similar stimulation of respiration was induced after T4 ghost attachment, showing that this process was independent of the mechanism of DNA injection. Rather, this change in the ...

journal_title：Journal of bacteriology

authors： Letellier L,Labedan B

更新日期：1985-01-01 00:00:00

abstract：:Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...

journal_title：Journal of bacteriology

authors： Willsky GR,Leung JO,Offermann PV Jr,Plotnick EK,Dosch SF

更新日期：1985-11-01 00:00:00

abstract：:The staphylococcal virulon is activated by the density-sensing agr system, which is autoinduced by a short peptide (autoinducing peptide [AIP]) processed from a propeptide encoded by agrD. A central segment of the agr locus, consisting of the C-terminal two-thirds of AgrB (the putative processing enzyme), AgrD, and th...

journal_title：Journal of bacteriology

authors： Jarraud S,Lyon GJ,Figueiredo AM,Lina G,Vandenesch F,Etienne J,Muir TW,Novick RP

更新日期：2000-11-01 00:00:00

abstract：:Replication initiator proteins in bacteria not only allow DNA replication but also often regulate the rate of replication initiation as well. The regulation is mediated by limiting the synthesis or availability of initiator proteins. The applicability of this principle is demonstrated here for RctB, the replication in...

journal_title：Journal of bacteriology

authors： Pal D,Venkova-Canova T,Srivastava P,Chattoraj DK

更新日期：2005-11-01 00:00:00

abstract：:The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...

journal_title：Journal of bacteriology

authors： Chen MW,Kotaka M,Vonrhein C,Bricogne G,Rao F,Chuah ML,Svergun D,Schneider G,Liang ZX,Lescar J

更新日期：2012-09-01 00:00:00

abstract：:The synthesis of pyruvate carboxylase (PC) was studied by using quantitative immunoblot analysis with an antibody raised against PC purified from Rhodobacter capsulatus and was found to vary 20-fold depending on the growth conditions. The PC content was high in cells grown on pyruvate or on carbon substrates metaboliz...

journal_title：Journal of bacteriology

authors： Yakunin AF,Hallenbeck PC

更新日期：1997-03-01 00:00:00

abstract：:Four genes encoding for polypeptides with apparent molecular weights of 17,000, 26,000 (the fimbrial subunit), 27,000, and 81,000 have been implicated in the biosynthesis of the K88ab fimbria (Mooi et al., J. Bacteriol. 150:512-521, 1982). Escherichia coli mutants with defects in these genes were examined for the pres...

journal_title：Journal of bacteriology

authors： Mooi FR,Wijfjes A,de Graaf FK

更新日期：1983-04-01 00:00:00

abstract：:Growth of Streptomyces viridochromogenes on a solid glycerol-NH4NO3 salts medium was accompanied by the formation of aerial mycelia and spores. Adding 0.5% or more casein hydrolysate to the medium stimulated growth while completely repressing the formation of aerial mycelia and spores. This repression was temporary, a...

journal_title：Journal of bacteriology

authors： Coleman RH,Ensign JC

更新日期：1982-03-01 00:00:00