Abstract:
:Four genes encoding for polypeptides with apparent molecular weights of 17,000, 26,000 (the fimbrial subunit), 27,000, and 81,000 have been implicated in the biosynthesis of the K88ab fimbria (Mooi et al., J. Bacteriol. 150:512-521, 1982). Escherichia coli mutants with defects in these genes were examined for the presence of fimbrial precursors. An analysis of these mutants revealed that fimbrial subunits accumulated transiently in the periplasmic space before being translocated across the outer membrane. The 81,000-dalton (d) polypeptide is probably involved in translocating fimbrial subunits across the outer membrane, because in the absence of this polypeptide the fimbrial subunits remained in the periplasmic space, where they were found to be associated with the 17,000- and 27,000-d polypeptides. In mutants with a deletion in the gene for the 27,000-d polypeptide, fimbrial precursors were not detected, because the fimbrial subunits were degraded. The 27,000-d polypeptide might be involved in stabilizing a conformation of the fimbrial subunit required to translocate it across the outer membrane. In the absence of the 17,000-d polypeptide, most fimbrial subunits were found in the periplasmic space associated with the 27,000-d polypeptide. However, small amounts of subunits were also translocated across the outer membrane. These extracellular subunits did not adhere to brushborders, suggesting that fimbrial subunits must be modified by the 17,000-d polypeptide to be assembled into functional fimbriae. A model for the biosynthesis of the K88ab fimbria is proposed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Mooi FR,Wijfjes A,de Graaf FKdoi
10.1128/JB.154.1.41-49.1983subject
Has Abstractpub_date
1983-04-01 00:00:00pages
41-9issue
1eissn
0021-9193issn
1098-5530journal_volume
154pub_type
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pub_type: 杂志文章
doi:10.1128/JB.145.1.145-155.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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doi:10.1128/jb.182.17.4951-4958.2000
更新日期:2000-09-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.82.1.130-134.1961
更新日期:1961-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.185.4.1289-1298.2003
更新日期:2003-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1213-1216.1973
更新日期:1973-06-01 00:00:00
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更新日期:1984-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.112.1.142-147.1972
更新日期:1972-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01047-07
更新日期:2007-11-01 00:00:00
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更新日期:1962-06-01 00:00:00
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更新日期:1969-04-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2005-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2010-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-05-01 00:00:00