Abstract:
:A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable from that of the parental strain, but they were unable to grow as mycelial forms after application of any of the common effective inducers, i.e., heat shock, pH alterations, proline addition, or use of GlcNAc as the carbon source. Studies performed with one selected strain demonstrated that it had severe alterations in the chemical composition of the cell wall, mainly in the levels of chitin and glucans, and in specific mannoproteins, some of them recognizable by specific polyclonal and monoclonal antibodies. It is suggested that these structural alterations hinder the construction of a normal hyphal wall.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Elorza MV,Sentandreu R,Ruiz-Herrera Jdoi
10.1128/jb.176.8.2318-2325.1994subject
Has Abstractpub_date
1994-04-01 00:00:00pages
2318-25issue
8eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.2178-2180.1990
更新日期:1990-04-01 00:00:00
abstract::The effects of guanosine tetraphosphate (ppGpp) and pyrimidine ribonucleoside triphosphates on Escherichia coli aspartate transcarbamylase (ATCase) synthesis were examined. To determine the effect of ppGpp, a stringent (relA+) and relaxed (relA) isogenic pair of E. coli K-12 strains was starved for isoleucine, and the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.998-1007.1983
更新日期:1983-02-01 00:00:00
abstract::The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.3.1306-1309.1972
更新日期:1972-12-01 00:00:00
abstract::Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.768-770.1982
更新日期:1982-02-01 00:00:00
abstract::Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3915-3923.1988
更新日期:1988-09-01 00:00:00
abstract::The extent of change in cytoplasmic proteins which accompanies yeast-to-mycelium morphogenesis of Candida albicans was analyzed by two-dimensional gel electrophoresis. Pure cultures of yeasts and true hyphae (i.e., without concomitant production of pseudohyphae) were grown in a synthetic low-sulfate medium. The two st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.258-273.1980
更新日期:1980-10-01 00:00:00
abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01690-09
更新日期:2010-05-01 00:00:00
abstract::A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1651-1655.1987
更新日期:1987-04-01 00:00:00
abstract::The presence of the altered lysine transfer ribonucleic acid (tRNA) in Bacillus subtilis spores is strongly dependent on the medium on which the cells were sporulated. Cells sporulated on synthetic media or dilute complex media contain little or none of the new component, whereas those sporulated on concentrated compl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.125-130.1967
更新日期:1967-07-01 00:00:00
abstract::Pseudomonas sp. strain LB400 grows on biphenyl as the sole carbon and energy source. This organism also cooxidizes several chlorinated biphenyl congeners. Biphenyl dioxygenase activity in cell extract required addition of NAD(P)H as an electron donor for the conversion of biphenyl to cis-2,3-dihydroxy-2,3-dihydrobiphe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.395-400.1993
更新日期:1993-01-01 00:00:00
abstract::The cell surfaces of several Lactobacillus species are covered by a regular layer composed of a single species of protein, the S-protein. The 43-kDa S-protein of the neotype strain Lactobacillus acidophilus ATCC 4356, which originated from the pharynx of a human, was purified. Antibodies generated against purified S-p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6089-6096.1993
更新日期:1993-10-01 00:00:00
abstract::Pathways of mannitol biosynthesis and utilization in Aspergillus candidus NRRL 305 were studied in cell-free extracts of washed mycelia prepared by sonic and French pressure cell treatments. A nicotinamide adenine dinucleotide-linked mannitol-1-phosphate (M1P) dehydrogenase was found in French pressure cell extracts o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1305-1309.1969
更新日期:1969-03-01 00:00:00
abstract::The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-13
更新日期:2013-08-01 00:00:00
abstract::The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3035-3040.1991
更新日期:1991-05-01 00:00:00
abstract::We previously showed that dprA is required for efficient processing of linear DNA during cellular transformation in Haemophilus influenzae. In this study the transcriptional regulation of dprA and two downstream genes, dprB and dprC, is examined. We demonstrate by Northern blot analysis that the dprABC genes are trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4815-4820.1997
更新日期:1997-08-01 00:00:00
abstract::Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.593-600.2005
更新日期:2005-01-01 00:00:00
abstract::Bacterial uptake of phosphate is usually accomplished via high-affinity transporters that are commonly regulated by two-component systems, which are activated when the concentration of phosphate is low. Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transpo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01965-14
更新日期:2014-10-01 00:00:00
abstract::Alginates are polysaccharides composed of 1-4-linked beta-D-mannuronic acid and alpha-L-guluronic acid. The polymer can be degraded by alginate lyases, which cleave the polysaccharide using a beta-elimination reaction. Two such lyases have previously been identified in the soil bacterium Azotobacter vinelandii, as fol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00455-09
更新日期:2009-08-01 00:00:00
abstract::A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.488-497.2005
更新日期:2005-01-01 00:00:00
abstract::Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.449-457.1968
更新日期:1968-02-01 00:00:00
abstract::The yeast-phase cell cycle of Wangiella dermatitidis was studied using flow microfluorimetry and the deoxyribonucleic acid (DNA) synthesis inhibitor hydroxyurea (HU). Exposure of exponential-phase yeastlike cells to 0.1 M HU for 3 to 6 h resulted in the arrest of the cells in DNA synthesis and produced a nearly homoge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.721-731.1980
更新日期:1980-11-01 00:00:00
abstract::Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.3.840-843.1974
更新日期:1974-09-01 00:00:00
abstract::Among mutants which require isoleucine, but not valine, for growth, we have found two distinguishable classes. One is defective in the biosynthetic enzyme threonine deaminase (l-threonine hydro-lyase, deaminating, EC 4.2.1.16) and the other has an altered isoleucyl transfer ribonucleic acid (tRNA) synthetase [l-isoleu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.2.527-537.1971
更新日期:1971-02-01 00:00:00
abstract::Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.486-494.1979
更新日期:1979-08-01 00:00:00
abstract::We have previously cloned the genes for synthesis of capsular polysaccharide (cps) and slime from Erwinia stewartii in cosmid pES2144. In this study, pES2144 was shown to complement 14 spontaneous cps mutants. These mutants were characterized by probing Southern blots of mutant genomic DNA with pES2144; insertions wer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.2.865-871.1988
更新日期:1988-02-01 00:00:00
abstract::Due in large part to their ability to facilitate the diffusion of a diverse range of solutes across the outer membrane (OM) of Gram-negative bacteria, the porins represent one of the most prominent and important bacterial membrane protein superfamilies. Notably, for the Gram-negative bacterium Desulfovibrio vulgaris H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00286-17
更新日期:2017-10-31 00:00:00
abstract::Many genomes of anaerobic hyperthermophiles encode multiple homologs of NAD(P)H oxidase that are thought to function in response to oxidative stress. We investigated one of the seven NAD(P)H oxidase homologs (TK1481) in the sulfur-reducing hyperthermophilic archaeon Thermococcus kodakarensis, focusing on the catalytic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00235-10
更新日期:2010-10-01 00:00:00
abstract::The F-plasmid-encoded TraI protein, also known as DNA helicase I, is a bifunctional protein required for conjugative DNA transfer. The enzyme catalyzes two distinct but functionally related reactions required for the DNA processing events associated with conjugation: the site- and strand-specific transesterification (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.697-706.2005
更新日期:2005-01-01 00:00:00
abstract::The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4288-4296.1991
更新日期:1991-07-01 00:00:00
abstract::Transcriptional regulation of the Mg(2+)-citrate transporter, CitM, the main citrate uptake system of Bacillus subtilis, was studied during growth in rich medium. Citrate in the growth medium was required for induction under all growth conditions. In Luria-Bertani medium containing citrate, citM expression was complet...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.854-859.2003
更新日期:2003-02-01 00:00:00