Crystal structure of PhnF, a GntR-family transcriptional regulator of phosphate transport in Mycobacterium smegmatis.

abstract：:Conjugative transfer and replacement of hundreds of kilobases of a bacterial chromosome can occur in vitro, but replacements in nature are either an order of magnitude smaller or involve the movement of mobile genetic elements. We discovered that two lineages of Staphylococcus aureus, including a pandemic methicillin-...

journal_title：Journal of bacteriology

authors： Robinson DA,Enright MC

更新日期：2004-02-01 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:Spectinomycin dependence in Bacillus subtilis involves two mutations, one conferring drug resistance and the other producing a requirement for spectinomycin for growth. ...

journal_title：Journal of bacteriology

authors： Henkin TM,Campbell KM,Chambliss GH

更新日期：1979-03-01 00:00:00

abstract：:Four regulated promoters that direct the transcription of genes (i.e., korA, tra, kilB, and korB) involved in the transfer of the Streptomyces plasmid pIJ101 were isolated following the in vitro fusion of plasmid DNA fragments to a promoterless gene encoding the S. lividans extracellular enzyme beta-galactosidase. Int...

journal_title：Journal of bacteriology

authors： Stein DS,Kendall KJ,Cohen SN

更新日期：1989-11-01 00:00:00

abstract：:The obligate intracellular pathogen Chlamydia trachomatis expresses a type III secretion system (T3SS) which has the potential to contribute significantly to pathogenesis. Based on a demonstrated role of type III secretion (T3S)-specific chaperones in the secretion of antihost proteins by gram-negative pathogens, we i...

journal_title：Journal of bacteriology

authors： Fields KA,Fischer ER,Mead DJ,Hackstadt T

更新日期：2005-09-01 00:00:00

abstract：:A strain of Escherichia coli which was derived from a gentamicin-resistant clinical isolate was found to be cross-resistant to neomycin and streptomycin. The molecular nature of the genetic defect was found to be an insertion of two GC base pairs in the uncG gene of the mutant. The insertion led to the production of a...

journal_title：Journal of bacteriology

authors： Humbert R,Altendorf K

更新日期：1989-03-01 00:00:00

abstract：:CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more...

journal_title：Journal of bacteriology

authors： Ohtake H,Cervantes C,Silver S

更新日期：1987-08-01 00:00:00

abstract：:Nitroheterocyclic and nitroaromatic compounds constitute an enormous range of chemicals whose potent biological activity has significant human health and environmental implications. The biological activity of nitro-substituted compounds is derived from reductive metabolism of the nitro moiety, a process catalyzed by a...

journal_title：Journal of bacteriology

authors： Whiteway J,Koziarz P,Veall J,Sandhu N,Kumar P,Hoecher B,Lambert IB

更新日期：1998-11-01 00:00:00

abstract：:The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

journal_title：Journal of bacteriology

authors： Yokota K,Kanamoto R,Kito M

更新日期：1980-03-01 00:00:00

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion m...

journal_title：Journal of bacteriology

authors： Cucarella C,Solano C,Valle J,Amorena B,Lasa I,Penadés JR

更新日期：2001-05-01 00:00:00

abstract：:The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...

journal_title：Journal of bacteriology

authors： Bedford DJ,Schweizer E,Hopwood DA,Khosla C

更新日期：1995-08-01 00:00:00

abstract：:Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the kn...

journal_title：Journal of bacteriology

authors： Willsky GR,Malamy MH

更新日期：1976-07-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:The interaction between endonuclease V, the cyclobutane pyrimidine dimer-specific N-glycosylase/abasic lyase from bacteriophage T4, and DNA was investigated by DNase I footprinting methods. The catalytically inactive mutant E23Q was found to interact with a smaller region of DNA at the abasic site analog, tetrahydrofu...

journal_title：Journal of bacteriology

authors： Latham KA,Manuel RC,Lloyd RS

更新日期：1995-09-01 00:00:00

abstract：:Aerobactin, a dihydroxamate siderophore produced by many strains of enteric bacteria, stimulated the growth of Neisseria gonorrhoeae FA19 and F62 in iron-limiting medium. However, gonococci did not produce detectable amounts of aerobactin in the Escherichia coli LG1522 aerobactin bioassay. We probed gonococcal genomic...

journal_title：Journal of bacteriology

authors： West SE,Sparling PF

更新日期：1987-08-01 00:00:00

abstract：:The diversity of regulatory systems encoded by bacteria provides an indication of the variety of stresses and interactions that these organisms encounter in nature. We have been investigating how the plant pathogen Pseudomonas syringae pv. tomato DC3000 responds to iron limitation and have focused on the iron starvati...

journal_title：Journal of bacteriology

authors： Markel E,Maciak C,Butcher BG,Myers CR,Stodghill P,Bao Z,Cartinhour S,Swingle B

更新日期：2011-10-01 00:00:00

abstract：:The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologo...

journal_title：Journal of bacteriology

authors： Godon JJ,Delorme C,Bardowski J,Chopin MC,Ehrlich SD,Renault P

更新日期：1993-07-01 00:00:00

abstract：:Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

journal_title：Journal of bacteriology

authors： Tisa LS,Ensign JC

更新日期：1988-07-01 00:00:00

abstract：:Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

journal_title：Journal of bacteriology

authors： Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

更新日期：1984-10-01 00:00:00

abstract：:The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...

journal_title：Journal of bacteriology

authors： Everett KD,Hatch TP

更新日期：1995-02-01 00:00:00

abstract：:The ferric enterobactin (FeEnt) receptor CfrA is present in the majority of Campylobacter jejuni isolates and is responsible for high-affinity iron acquisition. Our recent work and that of others strongly suggested the existence of another FeEnt uptake system in Campylobacter. Here we have identified and characterized...

journal_title：Journal of bacteriology

authors： Xu F,Zeng X,Haigh RD,Ketley JM,Lin J

更新日期：2010-09-01 00:00:00

abstract：:Strains of Lactobacillus casei subsp. rhamnosus possessing two cell wall polysaccharides, a hexosamine-containing H-polysaccharide and a rhamnose-containing R-polysaccharide, were examined for the effect of growth conditions on the production of these two components. In strain NCTC 6375, R- and H-polysaccharides accou...

journal_title：Journal of bacteriology

authors： Wicken AJ,Ayres A,Campbell LK,Knox KW

更新日期：1983-01-01 00:00:00

abstract：:Theophylline, aminophylline, and isobutylmethylxanthine, compounds reported to be inhibitors of adenosine 3',5'-phosphate (cAMP) phosphodiesterase, prevented the alpha-factor-induced cell cycle arrest of Saccharomyces cerevisiae a cells. To determine whether the in vivo effect of these methylxanthines on yeast pheromo...

journal_title：Journal of bacteriology

authors： Liao HH,Thorner J

更新日期：1981-12-01 00:00:00

abstract：:The outer membrane proteins (OMPs) of Gram-negative bacterial cells, as well as the mitochondrion and chloroplast organelles, possess unique and highly stable β-barrel structures. Biogenesis of OMPs in Escherichia coli involves such periplasmic chaperones as SurA and Skp. In this study, we found that the ΔsurA Δskp do...

journal_title：Journal of bacteriology

authors： Ge X,Lyu ZX,Liu Y,Wang R,Zhao XS,Fu X,Chang Z

更新日期：2014-02-01 00:00:00

abstract：:To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

journal_title：Journal of bacteriology

authors： Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

更新日期：1997-11-01 00:00:00

abstract：:The mechanisms by which bacteria uptake solutes across the cell membrane broadly impact their cellular energetics. Here, we use functional genomic, genetic, and biophysical approaches to reveal how Clostridium (Lachnoclostridium) phytofermentans, a model bacterium that ferments lignocellulosic biomass, uptakes plant h...

journal_title：Journal of bacteriology

authors： Cerisy T,Iglesias A,Rostain W,Boutard M,Pelle C,Perret A,Salanoubat M,Fierobe HP,Tolonen AC

更新日期：2019-07-10 00:00:00

abstract：UNLABELLED:Multiple essential small GTPases are involved in the assembly of the ribosome or in the control of its activity. Among them, ObgE (CgtA) has been shown recently to act as a ribosome antiassociation factor that binds to ppGpp, a regulator whose best-known target is RNA polymerase. The present study was aimed ...

journal_title：Journal of bacteriology

authors： Maouche R,Burgos HL,My L,Viala JP,Gourse RL,Bouveret E

更新日期：2016-06-13 00:00:00

abstract：:To examine the role of the amino acid residues (between positions 258 and 275 and positions 297 and 298) of the alpha-subunit of RNA polymerase in TyrR-mediated activation of the mtr promoter, we have carried out in vitro transcription experiments using a set of mutant RNA polymerases with a supercoiled mtr template. ...

journal_title：Journal of bacteriology

authors： Yang J,Murakami K,Camakaris H,Fujita N,Ishihama A,Pittard AJ

更新日期：1997-10-01 00:00:00

abstract：:phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from...

journal_title：Journal of bacteriology

authors： Pajunen MI,Kiljunen SJ,Söderholm ME,Skurnik M

更新日期：2001-03-01 00:00:00