Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli.

abstract：:Isolated cell walls of Staphylococcus aureus and Micrococcus lysodeikticus were found to expand and contract in response to changes in environmental pH and ionic strength. These volume changes, which could amount to as much as a doubling of wall dextran-impermeable volume, were related to changes in electrostatic inte...

journal_title：Journal of bacteriology

authors： Ou LT,Marquis RE

更新日期：1970-01-01 00:00:00

abstract：:Total DNA of various Rhizobium galegae strains representing different geographical origins, and taxonomic divergence was digested with three restriction enzymes separately, Southern blotted, and hybridized with six heterologous probes. The sequence divergences for different pairwise comparisons were calculated from pr...

journal_title：Journal of bacteriology

authors： Kaijalainen S,Lindström K

更新日期：1989-10-01 00:00:00

abstract：:The bacterial flagellum is a biological nanomachine that rotates to allow bacteria to swim. For flagellar rotation, torque is generated by interactions between a rotor and a stator. The stator, which is composed of MotA and MotB subunit proteins in the membrane, is thought to bind to the peptidoglycan (PG) layer, whic...

journal_title：Journal of bacteriology

authors： Zhu S,Nishikino T,Takekawa N,Terashima H,Kojima S,Imada K,Homma M,Liu J

更新日期：2020-01-29 00:00:00

abstract：:The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to prod...

journal_title：Journal of bacteriology

authors： Heffron JD,Sherry N,Popham DL

更新日期：2011-01-01 00:00:00

abstract：UNLABELLED:Our laboratory recently discovered that Escherichia coli cells starved for the DNA precursor dGTP are killed efficiently (dGTP starvation) in a manner similar to that described for thymineless death (TLD). Conditions for specific dGTP starvation can be achieved by depriving an E. coli optA1 gpt strain of the...

journal_title：Journal of bacteriology

authors： Itsko M,Schaaper RM

更新日期：2016-05-13 00:00:00

abstract：:Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...

journal_title：Journal of bacteriology

authors： Kim KS,Barksdale L

更新日期：1969-06-01 00:00:00

abstract：:Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the tolu...

journal_title：Journal of bacteriology

authors： Beller HR,Spormann AM

更新日期：1998-10-01 00:00:00

abstract：:The photosynthetic cyanobacterium Synechocystis sp. strain PCC6803 possesses homologs of known genes of the non-mevalonate 2-C-methyl-D-erythritol 2-phosphate (MEP) pathway for synthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Isoprenoid biosynthesis in extracts of this cyanobacterium, ...

journal_title：Journal of bacteriology

authors： Ershov YV,Gantt RR,Cunningham FX Jr,Gantt E

更新日期：2002-09-01 00:00:00

abstract：:PomA is thought to be a component of the ion channel in the sodium-driven polar-flagellar motor of Vibrio alginolyticus. We have found that some cysteine substitutions in the periplasmic region of PomA result in a slow-motility phenotype, in which swarming and swimming speeds are reduced even in the presence of high c...

journal_title：Journal of bacteriology

authors： Kojima S,Shoji T,Asai Y,Kawagishi I,Homma M

更新日期：2000-06-01 00:00:00

abstract：:Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...

journal_title：Journal of bacteriology

authors： Krebs MP,Khorana HG

更新日期：1993-03-01 00:00:00

abstract：:A Rhodobacter sphaeroides mutant (CYCA1) lacking cytochrome c2 (cyt c2) was previously constructed (T. J. Donohue, A. G. McEwan, S. Van Doren, A. R. Crofts, and S. Kaplan, Biochemistry, 27: 1918-1924, 1988) by a combination of in vivo and in vitro molecular genetic techniques. CYCA1 was incapable of photosynthetic gro...

journal_title：Journal of bacteriology

authors： Brandner JP,McEwan AG,Kaplan S,Donohue TJ

更新日期：1989-01-01 00:00:00

abstract：:We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

journal_title：Journal of bacteriology

authors： Rothstein DM,Magasanik B

更新日期：1980-02-01 00:00:00

abstract：:The PhoQ/PhoP two-component system is repressed by divalent cations, such as Mg(2+) and Ca(2+), in the growth medium and stimulated by low pH and certain cationic antimicrobial peptides. In Escherichia coli, it was recently shown that the histidine kinase PhoQ is also modulated by at least two additional factors, the ...

journal_title：Journal of bacteriology

authors： Lippa AM,Goulian M

更新日期：2012-03-01 00:00:00

abstract：:The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene enco...

journal_title：Journal of bacteriology

authors： Griggs DW,Tharp BB,Konisky J

更新日期：1987-12-01 00:00:00

abstract：:Peptidoglycan synthesis rate in nonconstricting filaments of Escherichia coli dnaX(Ts) has been studied by autoradiography of incorporated [3H]diaminopimelic acid. Analysis of autoradiograms of whole cells and sacculi showed that peptidoglycan is synthesized at a reduced rate in the nucleoid-containing parts of these ...

journal_title：Journal of bacteriology

authors： Mulder E,Woldringh CL

更新日期：1991-08-01 00:00:00

abstract：:Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...

journal_title：Journal of bacteriology

authors： Bizzini A,Zhao C,Budin-Verneuil A,Sauvageot N,Giard JC,Auffray Y,Hartke A

更新日期：2010-02-01 00:00:00

abstract：:The Escherichia coli K-12 hisT gene has been cloned, and its organization and expression have been analyzed on multicopy plasmids. The hisT gene, which encodes tRNA pseudouridine synthase I (PSUI), was isolated on a Clarke-Carbon plasmid known to contain the purF gene. The presence of the hisT gene on this plasmid was...

journal_title：Journal of bacteriology

authors： Marvel CC,Arps PJ,Rubin BC,Kammen HO,Penhoet EE,Winkler ME

更新日期：1985-01-01 00:00:00

abstract：:The allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. The implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organ...

journal_title：Journal of bacteriology

authors： Jensen RA,Stenmark SL

更新日期：1970-03-01 00:00:00

abstract：:A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS functions in rdgB mutants at the nonpermissive temperature. We identified the cloned gene as purA. ...

journal_title：Journal of bacteriology

authors： Clyman J,Cunningham RP

更新日期：1991-02-01 00:00:00

abstract：:We previously described 14 rare spontaneous morphological mutants of Candida albicans that were associated with chromosomal aberrations (E. P. Rustchenko-Bulgac, F. Sherman, and J. B. Hicks, J. Bacteriol. 172:1276-1283, 1990). Improved conditions for separation of chromosomes, as well as hybridization probes, were use...

journal_title：Journal of bacteriology

authors： Rustchenko-Bulgac EP

更新日期：1991-10-01 00:00:00

abstract：:plsB mutants of Escherichia coli are sn-glycerol 3-phosphate auxotrophs which owe their requirement to a K(m) defect in sn-glycerol 3-phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. We have located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene de...

journal_title：Journal of bacteriology

authors： Cronan JE Jr,Bell RM

更新日期：1974-10-01 00:00:00

abstract：:Salmonella typhimurium LT2 excreted under certain conditions an antibiotic substance designated typhimuricin. It is suggested that the LT2 "cryptic" plasmid is involved in its production and in the immunity to it. Preliminary characterization of typhimuricin is presented. ...

journal_title：Journal of bacteriology

authors： Ben-Gurion R,Flashner Y

更新日期：1982-10-01 00:00:00

abstract：:Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...

journal_title：Journal of bacteriology

authors： Alexeeva S,Hellingwerf KJ,Teixeira de Mattos MJ

更新日期：2002-03-01 00:00:00

abstract：:The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...

journal_title：Journal of bacteriology

authors： Jackson MP,Deresiewicz RL,Calderwood SB

更新日期：1990-06-01 00:00:00

abstract：:Integrons facilitate the evolution of complex phenotypes by physical and transcriptional linkage of genes. They can be categorized as chromosomal integrons (CIs) or mobile resistance integrons (MRIs). The significance of MRIs for the problem of multiple antibiotic resistance is well established. CIs are more widesprea...

journal_title：Journal of bacteriology

authors： Tetu SG,Holmes AJ

更新日期：2008-07-01 00:00:00

abstract：:Nitrogenase activities were determined from maximum acetylene reduction rates for mutant strains of Azotobacter vinelandii which are unable to fix N2 in the presence of molybdenum (Nif-) but undergo phenotypic reversal to Nif+ under conditions of Mo deficiency. The system responsible for N2 fixation under these condit...

journal_title：Journal of bacteriology

authors： Bishop PE,Jarlenski DM,Hetherington DR

更新日期：1982-06-01 00:00:00

abstract：:We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural ...

journal_title：Journal of bacteriology

authors： Hunke S,Landmesser H,Schneider E

更新日期：2000-03-01 00:00:00

abstract：:Horizontal gene transfer by conjugation plays a major role in bacterial evolution, allowing the acquisition of new traits, such as virulence and resistance to antibacterial agents. With the increased antibiotic resistance in bacterial pathogens, a better understanding of how bacteria modulate conjugation under changin...

journal_title：Journal of bacteriology

authors： Grace ED,Gopalkrishnan S,Girard ME,Blankschien MD,Ross W,Gourse RL,Herman C

更新日期：2015-03-01 00:00:00

abstract：:The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the me...

journal_title：Journal of bacteriology

authors： Hulbert RR,Taylor RK

更新日期：2002-10-01 00:00:00

abstract：:During meiosis and sporulation in Saccharomyces cerevisiae, the recessive genetic marker for cycloheximide resistance, believed to be due to an altered ribosomal protein (C. S. McLaughlin, p. 815-827, in M. Nomura et al., ed., Ribosomes, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.), is expressed as early a...

journal_title：Journal of bacteriology

authors： Emanuel JR,Magee PT

更新日期：1981-03-01 00:00:00