Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene.

abstract：:We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...

journal_title：Journal of bacteriology

authors： Freifelder D,Folkmanis A,Kirschner I

更新日期：1971-03-01 00:00:00

abstract：:Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the ...

journal_title：Journal of bacteriology

authors： FIRSHEIN W

更新日期：1961-08-01 00:00:00

abstract：:The suf operon is composed of four genes (sufB, sufC, sufD, and sufS) and is highly conserved in the genomes of cyanobacteria. Open reading frame sll0088 in Synechocystis sp. strain PCC 6803 is located near the 5' end of the suf operon but is transcribed in the direction opposite that of the suf operon. We previously ...

journal_title：Journal of bacteriology

authors： Wang T,Shen G,Balasubramanian R,McIntosh L,Bryant DA,Golbeck JH

更新日期：2004-02-01 00:00:00

abstract：:Some strains of Streptomyces produce sialidases. Two sialidases were purified over 1,000-fold from a culture filtrate of two Streptomyces species. They had the same properties in molecular weight, behavior to ions and other reagents, and substrate specificity. They showed very small differences in kinetic properties, ...

journal_title：Journal of bacteriology

authors： Kunimoto S,Aoyagi T,Takeuchi T,Umezawa H

更新日期：1974-08-01 00:00:00

abstract：:Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...

journal_title：Journal of bacteriology

authors： Estrada-Parra S,Zamora A,Bojalil LF

更新日期：1965-09-01 00:00:00

abstract：:Glycerol:NAD+2-OXIDOREDUCTASE (EC 1.1.1.6) was purified to homogeneity from a mutant of Escherichia coli K12 that uses this enzyme, instead of ATP:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sul...

journal_title：Journal of bacteriology

authors： Tang CT,Ruch FE Jr,Lin CC

更新日期：1979-10-01 00:00:00

abstract：:We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...

journal_title：Journal of bacteriology

authors： Eggers CH,Kimmel BJ,Bono JL,Elias AF,Rosa P,Samuels DS

更新日期：2001-08-01 00:00:00

abstract：:When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a beta-galactosidase gene transcriptionally fused to the inactivated target gene, was used to ide...

journal_title：Journal of bacteriology

authors： Prágai Z,Eschevins C,Bron S,Harwood CR

更新日期：2001-04-01 00:00:00

abstract：:A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1, 4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA g...

journal_title：Journal of bacteriology

authors： Suvarna K,Stevenson D,Meganathan R,Hudspeth ME

更新日期：1998-05-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:Human dental biofilm communities comprise several species, which can interact cooperatively or competitively. Bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. Lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of ...

journal_title：Journal of bacteriology

authors： Periasamy S,Kolenbrander PE

更新日期：2010-06-01 00:00:00

abstract：:A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade thes...

journal_title：Journal of bacteriology

authors： Chaudhry GR,Huang GH

更新日期：1988-09-01 00:00:00

abstract：:Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...

journal_title：Journal of bacteriology

authors： Tsai HF,Chang YC,Washburn RG,Wheeler MH,Kwon-Chung KJ

更新日期：1998-06-01 00:00:00

abstract：:We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...

journal_title：Journal of bacteriology

authors： McDonough KA,Hare JM

更新日期：1997-03-01 00:00:00

abstract：:Carbon catabolite repression of maltase gene expression is brought about by the addition of glucose, resulting in a drastic inhibition of the induction of maltase. When added to induced cells, glucose leads to the inhibition of maltase synthesis within 30 min, which can be accounted for by the disappearance of hybridi...

journal_title：Journal of bacteriology

authors： Federoff HJ,Eccleshall TR,Marmur J

更新日期：1983-10-01 00:00:00

abstract：:A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four muta...

journal_title：Journal of bacteriology

authors： Van Alstyne D,Simon MI

更新日期：1971-12-01 00:00:00

abstract：:A survey of chromosomal variation in the ST239 clonal group of methicillin-resistant Staphylococcus aureus (MRSA) revealed a novel genetic element, ICE6013. The element is 13,354 bp in length, excluding a 6,551-bp Tn552 insertion. ICE6013 is flanked by 3-bp direct repeats and is demarcated by 8-bp imperfect inverted r...

journal_title：Journal of bacteriology

authors： Smyth DS,Robinson DA

更新日期：2009-10-01 00:00:00

abstract：:The gene GK3045 (741 bp) from Geobacillus kaustophilus HTA426 was cloned, sequenced, and overexpressed into Escherichia coli Rosetta (DE3). The deduced protein was a 30-kDa monomeric esterase with high homology to carboxylesterases from Geobacillus thermoleovorans NY (99% identity) and Geobacillus stearothermophilus (...

journal_title：Journal of bacteriology

authors： Montoro-García S,Martínez-Martínez I,Navarro-Fernández J,Takami H,García-Carmona F,Sánchez-Ferrer A

更新日期：2009-05-01 00:00:00

abstract：:Heterocyst differentiation is orchestrated by the N control transcriptional regulator NtcA and the differentiation-specific factor HetR. In Anabaena sp. strain PCC 7120, the devBCA operon is expressed from two different promoters activated upon N stepdown. The distal devB promoter (transcription start point [TSP] loca...

journal_title：Journal of bacteriology

authors： Camargo S,Valladares A,Flores E,Herrero A

更新日期：2012-06-01 00:00:00

abstract：:Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.1a and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and ou...

journal_title：Journal of bacteriology

authors： Seale T

更新日期：1973-02-01 00:00:00

abstract：:We previously showed that the Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica (Y. Irie, S. Mattoo, and M. H. Yuk, J. Bacteriol. 186:5692-5698, 2004). Analyses of the extracellular components of B. bronchiseptica biofilm matrix revealed that the major sugar component in the matrix ...

journal_title：Journal of bacteriology

authors： Irie Y,Preston A,Yuk MH

更新日期：2006-09-01 00:00:00

abstract：:We have utilized transposon mutagenesis to obtain insertional mutations in Providencia stuartii that activate the chromosomal aac(2')-la gene. Two closely linked mini-Tn5Cm insertions were obtained in a locus designated aarA, and a single insertion was obtained in a separate locus, aarC. Nucleotide sequence analysis, ...

journal_title：Journal of bacteriology

authors： Rather PN,Orosz E

更新日期：1994-08-01 00:00:00

abstract：:The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...

journal_title：Journal of bacteriology

authors： Gyohda A,Funayama N,Komano T

更新日期：1997-03-01 00:00:00

abstract：:Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...

journal_title：Journal of bacteriology

authors： Lei MG,Cue D,Roux CM,Dunman PM,Lee CY

更新日期：2011-10-01 00:00:00

abstract：:Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...

journal_title：Journal of bacteriology

authors： Noriea NF,Clark TR,Mead D,Hackstadt T

更新日期：2017-02-28 00:00:00

abstract：:Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...

journal_title：Journal of bacteriology

authors： Stubbendieck RM,Straight PD

更新日期：2016-07-28 00:00:00

abstract：:UDP-N-acetylmuramic acid:L-alanine ligase (MurC) catalyzes the addition of the first amino acid to the cytoplasmic precursor of the bacterial cell wall peptidoglycan. The crystal structures of Haemophilus influenzae MurC in complex with its substrate UDP-N-acetylmuramic acid (UNAM) and Mg(2+) and of a fully assembled ...

journal_title：Journal of bacteriology

authors： Mol CD,Brooun A,Dougan DR,Hilgers MT,Tari LW,Wijnands RA,Knuth MW,McRee DE,Swanson RV

更新日期：2003-07-01 00:00:00

abstract：:A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis. ...

journal_title：Journal of bacteriology

authors： Atherly AG

更新日期：1974-06-01 00:00:00

abstract：:Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...

journal_title：Journal of bacteriology

authors： Miller RV,Kokjohn TA

更新日期：1988-05-01 00:00:00

abstract：:This study examined the catabolism of xanthone by an Arthrobacter sp. (strain GFB100) capable of growth on xanthone as its main source of carbon and energy. An early catabolic intermediate was 3,4-dihydroxyxanthone. This compound was isolated from the growth medium of a mutant strain of the Arthrobacter sp. which lack...

journal_title：Journal of bacteriology

authors： Tomasek PH,Crawford RL

更新日期：1986-09-01 00:00:00