Abstract:
:We have utilized transposon mutagenesis to obtain insertional mutations in Providencia stuartii that activate the chromosomal aac(2')-la gene. Two closely linked mini-Tn5Cm insertions were obtained in a locus designated aarA, and a single insertion was obtained in a separate locus, aarC. Nucleotide sequence analysis, complementation studies, and localization of the sites of mini-Tn5Cm insertion have allowed the identification of the aarA coding region. The deduced AarA protein had a molecular mass of 31,086 kDa and displayed characteristics of an integral membrane protein. A strain deleted for the aarA gene by allelic exchange showed at least a fourfold increase in the accumulation of aac(2')-la mRNA and an eightfold increase in aminoglycoside resistance. Mutations in aarA were pleiotrophic and also resulted in loss of pigmentation and a deficiency in cell separation during division.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rather PN,Orosz Edoi
10.1128/jb.176.16.5140-5144.1994subject
Has Abstractpub_date
1994-08-01 00:00:00pages
5140-4issue
16eissn
0021-9193issn
1098-5530journal_volume
176pub_type
杂志文章abstract::Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.333-341.1979
更新日期:1979-11-01 00:00:00
abstract::Three species of Desulfovibrio were found to have a high degree of ribosomal ribonucleic acid homology with Desulfovibrio vulgaris. Desulfotomaculum nigrificans, which is also a sulfate-reducing anaerobe, had only 38% ribosomal ribonucleic acid homology with D. vulgaris. The homologies of six other unrelated genera we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.717-719.1971
更新日期:1971-06-01 00:00:00
abstract::Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.18.4952-4961.2002
更新日期:2002-09-01 00:00:00
abstract::Mutants of Anabaena sp. strain PCC 7120 that are incapable of sustained growth with air as the sole source of nitrogen were generated by using Tn5-derived transposons. Nitrogenase was expressed only in mutants that showed obvious morphological signs of heterocyst differentiation. Even under rigorously anaerobic condit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6025-6032.1992
更新日期:1992-10-01 00:00:00
abstract::Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.1.168-174.2005
更新日期:2005-01-01 00:00:00
abstract::A specialized transducing phage lambda carrying the structural gene for the OmpF protein, an outer membrane matrix protein, was isolated. The phage carries the 20.5--21-min region of the Escherichia coli K-12 chromosome and carries asnS, ompF, and aspC genes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.1085-1090.1981
更新日期:1981-02-01 00:00:00
abstract::Mycoplasma pneumoniae cytadherence is mediated by a specialized, polar attachment organelle. Certain spontaneously arising cytadherence mutants (designated class I) lack HMW2, fail to localize the adhesin protein P1 to the attachment organelle, and exhibit accelerated turnover of proteins HMW1, HMW3, and P65. Insertio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.14.4404-4410.1999
更新日期:1999-07-01 00:00:00
abstract::Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.729-731.1986
更新日期:1986-08-01 00:00:00
abstract::The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2385-2393.1999
更新日期:1999-04-01 00:00:00
abstract::Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.1.251-258.1993
更新日期:1993-01-01 00:00:00
abstract::The participation of tyramine oxidase in the regulation of arylsulfatase synthesis in Salmonella typhimurium was studied. Arylsulfatase synthesis was repressed by inorganic sulfate, cysteine, methionine, or taurine. This repression was relieved by tyramine, octopamine, or dopamine, which induced tyramine oxidase synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.796-802.1981
更新日期:1981-02-01 00:00:00
abstract::Aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (PQQ) as a prosthetic group. Seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with PQQ have been isolated in the facultative methanol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.6.1746-1755.1994
更新日期:1994-03-01 00:00:00
abstract::In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3127-3132.1996
更新日期:1996-06-01 00:00:00
abstract::Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells at 30 degrees C but grew as spherical cells at 42 degrees C. The phenotype of one of these rod(Ts) mutants was 88% cotran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.1143-1158.1978
更新日期:1978-12-01 00:00:00
abstract::With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1228-1233.1986
更新日期:1986-12-01 00:00:00
abstract::Mycobacterium tuberculosis can persist for many years within host lung tissue without causing clinical disease. Little is known about the state in which the bacilli survive, although it is frequently referred to as dormancy. Some evidence suggests that cells survive in nutrient-deprived stationary phase. Therefore, we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.270-283.1999
更新日期:1999-01-01 00:00:00
abstract::A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.11.3197-3203.2000
更新日期:2000-06-01 00:00:00
abstract::The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.15.4997-5004.1992
更新日期:1992-08-01 00:00:00
abstract::Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to par...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.5.1705-1721.1967
更新日期:1967-05-01 00:00:00
abstract::The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.1.250-258.1968
更新日期:1968-07-01 00:00:00
abstract::The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.7.1864-1871.2000
更新日期:2000-04-01 00:00:00
abstract::Thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. The substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.3.874-878.1967
更新日期:1967-03-01 00:00:00
abstract::The endoplasmic reticulum from Neurospora crassa was identified by monitoring the activity of the putative enzyme marker phosphatidylcholine glyceride transferase. After differential centrifugation of a cell homogenate, phosphatidylcholine glyceride transferase activity initially copurified with plasma membrane H+-ATP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.362-368.1983
更新日期:1983-10-01 00:00:00
abstract::Aerobic microorganisms have evolved different strategies to withstand environmental oxidative stresses generated by various reactive oxygen species (ROS). For the facultative anaerobic human oral pathogen Streptococcus mutans, the mechanisms used to protect against ROS are not fully understood, since it does not posse...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00184-09
更新日期:2009-07-01 00:00:00
abstract::In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.4.987-991.1995
更新日期:1995-02-01 00:00:00
abstract::Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.4.1028-1035.1966
更新日期:1966-10-01 00:00:00
abstract::The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.426-433.1982
更新日期:1982-02-01 00:00:00
abstract::Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.3.1022-1035.2005
更新日期:2005-02-01 00:00:00
abstract::Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1955-1961.1996
更新日期:1996-04-01 00:00:00
abstract::The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.22.6728-6731.2003
更新日期:2003-11-01 00:00:00