N4 RNA polymerase II, a heterodimeric RNA polymerase with homology to the single-subunit family of RNA polymerases.

Abstract:

:Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Willis SH,Kazmierczak KM,Carter RH,Rothman-Denes LB

doi

10.1128/jb.184.18.4952-4961.2002

keywords:

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

4952-61

issue

18

eissn

0021-9193

issn

1098-5530

journal_volume

184

pub_type

杂志文章