Abstract:
:Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Willis SH,Kazmierczak KM,Carter RH,Rothman-Denes LBdoi
10.1128/jb.184.18.4952-4961.2002keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
4952-61issue
18eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.852-854.1985
更新日期:1985-05-01 00:00:00
abstract::A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5877-5883.1990
更新日期:1990-10-01 00:00:00
abstract::A lipopolysaccharide (LPS) has been isolated from the gram-negative photosynthetic bacterium Rhodopseudomonas capsulata. Chemical analysis revealed the presence of d-glucose, d-galactose, l-rhamnose, 3-O-methyl-l-rhamnose (l-acofriose), d-glucosamine, 2-keto-3-deoxyoctonate, and neuraminic acid. The LPS does not conta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.3.1106-1113.1972
更新日期:1972-03-01 00:00:00
abstract::Billen, Daniel (University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Tex.), and Roger Hewitt. Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J. Bacteriol. 92:609-617. 1966.-A study has been made of the subsequent replicative ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.3.609-617.1966
更新日期:1966-09-01 00:00:00
abstract::Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06584-11
更新日期:2012-02-01 00:00:00
abstract::We previously showed that the Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica (Y. Irie, S. Mattoo, and M. H. Yuk, J. Bacteriol. 186:5692-5698, 2004). Analyses of the extracellular components of B. bronchiseptica biofilm matrix revealed that the major sugar component in the matrix ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00605-06
更新日期:2006-09-01 00:00:00
abstract::RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8499-8507.2004
更新日期:2004-12-01 00:00:00
abstract::Resting cells of Escherichia coli are able to initiate growth and murein biosynthesis in the presence of beta-lactam antibiotics binding to penicillin-binding proteins (PBPs) 1a and 1b (E. J. de la Rosa, M. A. de Pedro, and D. Vázquez, Proc. Natl. Acad. Sci. USA 82:5632-5635, 1985). Under these conditions, cells elong...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2410-2416.1987
更新日期:1987-06-01 00:00:00
abstract::Purified suspensions of Chlamydia psittaci were prepared from L cells. Thin sections of intact elementary bodies and intact developmental reticulate bodies and of their purified envelopes were observed by electron microscopy. In both intact organisms and partially purified envelopes, two membranous structures, each ap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.355-360.1971
更新日期:1971-01-01 00:00:00
abstract::The structural properties required for the binding of peptide substrates to the Escherichia coli periplasmic protein involved in oligopeptide transport were surveyed by measuring the ability of different peptides to compete for binding in an equilibrium dialysis assay with the tripeptide Ala-Phe-[3H]Gly. The protein s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.775-779.1986
更新日期:1986-11-01 00:00:00
abstract::Mycoplasma iowae is associated mainly with reduced hatchability in turkeys and is well known for the unusual ability of phenotypic variation in the Mycoplasma surface components as well as a relative resistance to heat, bile salts, and many antimicrobials. A subset of unique genes and a gene cluster responsible for th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06297-11
更新日期:2012-01-01 00:00:00
abstract::The deletion of a gene coding for a histidine kinase (sll0750, Hik8) in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 resulted in a conditional lethal phenotype with a pleiotropic effect on the expression of genes involved in glucose metabolism. This mutant had comparable doubling times to wild type...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2368-2376.2005
更新日期:2005-04-01 00:00:00
abstract::rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.20.6185-6191.2003
更新日期:2003-10-01 00:00:00
abstract::From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.2.284-290.1994
更新日期:1994-01-01 00:00:00
abstract::The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.20.5885-5892.2000
更新日期:2000-10-01 00:00:00
abstract::We present experimental evidence for the existence of multiple activator-binding sites in the upstream sequence of the ompC promoter, the expression of which is activated by the positive regulator OmpR in response to the osmolarity of the medium. We also found that a single OmpR-binding site can activate the ompC prom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.501-503.1990
更新日期:1990-01-01 00:00:00
abstract::Protein and ribonucleic acid (RNA) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. Immediately after heating, cells were incubated in growth media containing either [14C]leucine or [3H]uracil. The labeled cells were subsequently treated with pen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.492-498.1979
更新日期:1979-05-01 00:00:00
abstract::The model of pneumonia produced by intranasal inoculation of Mycoplasma pulmonis in gnotobiotic mice provided the opportunity to study the localization and identification of the infecting organisms in the tissues by immunofluorescence techniques. Frozen sections of pneumonic mouse lung were fixed in acetone, layered w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.1.250-258.1968
更新日期:1968-07-01 00:00:00
abstract::The butyrogenic genes from Clostridium difficile DSM 1296(T) have been cloned and expressed in Escherichia coli. The enzymes acetyl-coenzyme A (CoA) C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, phosphate butyryltransferase, and butyrate kinase and the butyryl-CoA dehydrogenase complex composed o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00321-13
更新日期:2013-08-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium that lacks the DNA adenine methylase (Dam) ectopically expresses multiple genes that are preferentially expressed during infection, is attenuated for virulence, and confers heightened immunity in vaccinated hosts. The safety of dam mutant Salmonella vaccines was evaluated by scr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00299-07
更新日期:2007-07-01 00:00:00
abstract::We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.9.2711-2722.2003
更新日期:2003-05-01 00:00:00
abstract::The Bacillus subtilis enzymes ExoA and Nfo (originally termed YqfS) are endonucleases that can repair apurinic/apyrimidinic (AP) sites and strand breaks in DNA. We have analyzed how the lack of ExoA and Nfo affects the resistance of growing cells and dormant spores of B. subtilis to a variety of treatments, some of wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7374-7381.2005
更新日期:2005-11-01 00:00:00
abstract::The biological activities of Bacillus subtilis ribosomes pretreated with 0.5, 1, or 2 mmp-chloromercuribenzoate (PCMB) were examined. The ribosomes treated with 1 or 2 mm PCMB lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. On the other hand, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.188-194.1972
更新日期:1972-10-01 00:00:00
abstract::We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.11.3562-3570.1999
更新日期:1999-06-01 00:00:00
abstract::Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intrace...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01997-07
更新日期:2008-04-01 00:00:00
abstract::The promoter selectivity of two extracytoplasmic function (ECF) subfamily sigma subunits, sigma(E) (sigma(24)) and sigma(FecI) (sigma(18)), of Escherichia coli RNA polymerase was analyzed by using an in vitro transcription system and various promoters. The Esigma(E) holoenzyme recognized only the known cognate promote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.4.1181-1184.2000
更新日期:2000-02-01 00:00:00
abstract::A deoxyribonucleic acid-directed in vitro system for the synthesis of Escherichia coli carbamoylphosphate synthase has been developed, and its properties have been studied. The system uses the deoxyribonucleic acid of a lambda phage carrying the car genes (lambdadcarAB) as template and mediates the synthesis of both s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.58-66.1980
更新日期:1980-01-01 00:00:00
abstract::The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.10.3551-3571.2006
更新日期:2006-05-01 00:00:00
abstract::During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.18.6198-6207.2004
更新日期:2004-09-01 00:00:00
abstract::During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.1151-1154.1990
更新日期:1990-02-01 00:00:00