Protein and ribonucleic acid syntheses in heat-damaged and heat-killed Escherichia coli.

abstract：:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

journal_title：Journal of bacteriology

authors： Livingston DM,Klein HL

更新日期：1977-01-01 00:00:00

abstract：:By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...

journal_title：Journal of bacteriology

authors： Tucker SD,Gopalakrishnan AS,Bollinger R,Dowhan W,Murgola EJ

更新日期：1982-11-01 00:00:00

abstract：:Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...

journal_title：Journal of bacteriology

authors： Linn T,Goman M,Scaife J

更新日期：1979-11-01 00:00:00

abstract：:Rebers, P. A. (Rutgers, The State University, New Brunswick, N. J.), E. Hurwitz, M. Heidelberger, and S. Estrada-Parra. Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI. J. Bacteriol. 83:335-342. 1962.-The type-specific pneumococcal...

journal_title：Journal of bacteriology

authors： REBERS PA,HURWITZ E,HEIDELBERGER M,ESTRADA-PARRA S

更新日期：1962-02-01 00:00:00

abstract：:During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...

journal_title：Journal of bacteriology

authors： Wesp A,Prinz S,Fink GR

更新日期：2001-04-01 00:00:00

abstract：:The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...

journal_title：Journal of bacteriology

authors： Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

更新日期：1988-10-01 00:00:00

abstract：:Escherichia coli strains mutant in the starvation gene cstC grow normally in a mineral salts medium but are impaired in utilizing amino acids as nitrogen sources. They are also compromised in starvation survival, where amino acid catabolism is important. The cstC gene encodes a 406-amino-acid protein that closely rese...

journal_title：Journal of bacteriology

authors： Fraley CD,Kim JH,McCann MP,Matin A

更新日期：1998-08-01 00:00:00

abstract：:A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could...

journal_title：Journal of bacteriology

authors： Ray PH,Benedict CD

更新日期：1980-04-01 00:00:00

abstract：:Mutants of Escherichia coli K-12 constitutive for fatty acid degradation (fadR) showed an increased rate of utilization of exogenous acetate. Acetate transport, oxidation, and incorporation into macromolecules was approximately fivefold greater in fadR mutants than fadR+ strains during growth on succinate as a carbon ...

journal_title：Journal of bacteriology

authors： Maloy SR,Nunn WD

更新日期：1981-10-01 00:00:00

abstract：:Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...

journal_title：Journal of bacteriology

authors： Benning C,Somerville CR

更新日期：1992-10-01 00:00:00

abstract：:Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...

journal_title：Journal of bacteriology

authors： Amikam D,Kuhn J

更新日期：1987-05-01 00:00:00

abstract：:We previously described 14 rare spontaneous morphological mutants of Candida albicans that were associated with chromosomal aberrations (E. P. Rustchenko-Bulgac, F. Sherman, and J. B. Hicks, J. Bacteriol. 172:1276-1283, 1990). Improved conditions for separation of chromosomes, as well as hybridization probes, were use...

journal_title：Journal of bacteriology

authors： Rustchenko-Bulgac EP

更新日期：1991-10-01 00:00:00

abstract：:ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...

journal_title：Journal of bacteriology

authors： Tanaka S,Lee SO,Hamaoka K,Kato J,Takiguchi N,Nakamura K,Ohtake H,Kuroda A

更新日期：2003-09-01 00:00:00

abstract：:We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...

journal_title：Journal of bacteriology

authors： Walderhaug MO,Litwack ED,Epstein W

更新日期：1989-02-01 00:00:00

abstract：:In the long-chain n-alkane degrader Acinetobacter sp. strain M-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed con...

journal_title：Journal of bacteriology

authors： Tani A,Ishige T,Sakai Y,Kato N

更新日期：2001-03-01 00:00:00

abstract：:After thermal denaturation, an in vivo-labeled RNA was found in a temperature-sensitive initiation mutant of Bacillus subtilis (dna-37) associated with high-molecular-weight DNA. This RNA could be clearly distinguished from other RNA species by different techniques of separation, such as Sepharose 2B filtration, chrom...

journal_title：Journal of bacteriology

authors： Henckes G,Vannier F,Buu A,Seror-Laurent SJ

更新日期：1982-01-01 00:00:00

abstract：:Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...

journal_title：Journal of bacteriology

authors： Lindgren PB,Peet RC,Panopoulos NJ

更新日期：1986-11-01 00:00:00

abstract：:Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...

journal_title：Journal of bacteriology

authors： Satta G,Fontana R,Canepari P,Botta G

更新日期：1979-02-01 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...

journal_title：Journal of bacteriology

authors： Eisenstadt E,Fisher S,Der CL,Silver S

更新日期：1973-03-01 00:00:00

abstract：:Vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. Growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. Mutants, created by transposon mutage...

journal_title：Journal of bacteriology

authors： McCarter LL,Wright ME

更新日期：1993-06-01 00:00:00

abstract：:Sequences of the 16S ribosomal DNA (rDNA) from psychrotolerant and mesophilic strains of the Bacillus cereus group revealed signatures which were specific for these two thermal groups of bacteria. Further analysis of the genomic DNA from a wide range of food and soil isolates showed that B. cereus group strains have b...

journal_title：Journal of bacteriology

authors： Prüss BM,Francis KP,von Stetten F,Scherer S

更新日期：1999-04-01 00:00:00

abstract：:The cheA locus of Escherichia coli encodes two similar proteins, CheAL (654 amino acids) and CheAS (557 amino acids), which are made by initiating translation from different in-frame start sites [start(L) and start(S)]. CheAL plays an essential role in chemotactic signaling. It autophosphorylates at a histidine residu...

journal_title：Journal of bacteriology

authors： Sanatinia H,Kofoid EC,Morrison TB,Parkinson JS

更新日期：1995-05-01 00:00:00

abstract：:Direct evidence for the involvement of ubiquinone in H2 oxidation by Rhizobium japonicum was demonstrated; H2 reduced ca. 80% of the extractable ubiquinone. The inhibitor 2-n-heptyl-4-hydroxyquinoline-N-oxide blocked electron transport at a site between ubiquinone and the cytochromes. The results showed that no cytoch...

journal_title：Journal of bacteriology

authors： O'Brian MR,Maier RJ

更新日期：1985-02-01 00:00:00

abstract：:The xylanase XynC of Fibrobacter succinogenes S85 was recently shown to contain three distinct domains, A, B, and C (F. W. Paradis, H. Zhu, P. J. Krell, J. P. Phillips, and C. W. Forsberg, J. Bacteriol. 175:7666-7672, 1993). Domains A and B each bear an active site capable of hydrolyzing xylan, while domain C has no e...

journal_title：Journal of bacteriology

authors： Zhu H,Paradis FW,Krell PJ,Phillips JP,Forsberg CW

更新日期：1994-07-01 00:00:00

abstract：:Galactose-negative mutants of the group H Streptococcus strain Challis were obtained by treatment with nitrosoguanidine. Enzyme assays of extracts of these mutants revealed that 12 of the mutants were lacking one of the enzymes of the Leloir pathway. Thus, the Leloir pathway is the major means of galactose metabolism ...

journal_title：Journal of bacteriology

authors： Luginbuhl GH,Gooder H

更新日期：1972-02-01 00:00:00

abstract：:In previous work, we cloned a Candida albicans cDNA for the glycolytic enzyme enolase and found a single, abundant enolase transcript on Northern (RNA) blots and a single protein on immunoblots, using antiserum raised against a recombinant enolase fusion protein. Because C. albicans enolase is abundantly produced duri...

journal_title：Journal of bacteriology

authors： Postlethwait P,Sundstrom P

更新日期：1995-04-01 00:00:00

abstract：:The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...

journal_title：Journal of bacteriology

authors： Schäferkordt S,Chakraborty T

更新日期：1997-04-01 00:00:00

abstract：:Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated ou...

journal_title：Journal of bacteriology

authors： Bosch R,Moore ER,García-Valdés E,Pieper DH

更新日期：1999-04-01 00:00:00

abstract：:Chloroflexus, a newly described genus of filamentous, photosynthetic, gliding bacteria, oxidizes sulfide anaerobically under photoautotrophic or photoheterotrophic growh conditions and deposits elemental sulfur outside the cell. The formation of sulfur granules outside the cell supports the idea that this organism is ...

journal_title：Journal of bacteriology

authors： Madigan MT,Brock TD

更新日期：1975-05-01 00:00:00