Abstract:
:Vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. Growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. Mutants, created by transposon mutagenesis of a clone expressing lateral flagellin and gene disruption in V. parahaemolyticus, were unable to swarm and failed to make lateral flagellin; therefore, unlike the case for the polar system, there is one gene (lafA) encoding lateral flagellin. In addition to lafA, other genes required for swarming but not for swimming were identified by gene replacement mutagenesis. The nucleotide sequence of the clone determined open reading frames (ORFs) and deduced amino acid sequences showed similarities to flagellar components of other bacteria: flagellin, hook-associated protein (HAP2), motor components, and flagellar sigma factor (sigma 28). Many sigma 28 factors have been shown to recognize cognate promoters; however, expression of lafA in Escherichia coli required LafS, and E. coli sigma 28 did not substitute. Also, there were no sequences preceding genes encoding flagellin or HAP2 resembling the sigma 28 consensus promoter. The product of the sigma-like gene seems to be a unique member of the sigma 28 cluster. It appears the result of requiring expression for immunodetection of flagellin clones was that the sigma locus was fortuitously cloned, since the sigma and lafA loci were not contiguous in the chromosome. This work initiates identification and placement of genes in a scheme of control for swarmer cell differentiation; three levels have been identified in the transcriptional hierarchy.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
McCarter LL,Wright MEdoi
10.1128/jb.175.11.3361-3371.1993subject
Has Abstractpub_date
1993-06-01 00:00:00pages
3361-71issue
11eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract::The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.15.4593-4602.2003
更新日期:2003-08-01 00:00:00
abstract::Escherichia coli K-12 produces at least two ATP-dependent proteases, Lon (La) and Clp (Ti), the latter consisting of a regulatory subunit (ClpA) and a proteolytic subunit (ClpP). The gene clpB encoding an analog of ClpA had been found at 57 min on the E. coli chromosome. Cloning and examination of novel heat shock pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.14.4247-4253.1991
更新日期:1991-07-01 00:00:00
abstract::Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-tre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.3.732-739.1986
更新日期:1986-03-01 00:00:00
abstract::trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and transl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4272-4278.1995
更新日期:1995-08-01 00:00:00
abstract::Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.4137-4139.1995
更新日期:1995-07-01 00:00:00
abstract::Monoubiquitination of the 12-transmembrane segment (12-TMS) Saccharomyces cerevisiae maltose transporter promoted the maximal internalization rate of this protein. This modification is similar to that of the 7-TMS alpha-factor receptor but different from that of the 12-TMS uracil and general amino acid permeases. This...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.241-243.2000
更新日期:2000-01-01 00:00:00
abstract::Catabolism of nonphenolic arenes is frequently initiated by dioxygenases, yielding single isomer products with two adjacent hydroxylated asymmetric centers. The next enzymic reaction dehydrogenates these cyclic cis-diols, with aromatization yielding catechols for ring cleavage. There are two stereochemical questions t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.4023-4029.1997
更新日期:1997-06-01 00:00:00
abstract::MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3151-3158.2002
更新日期:2002-06-01 00:00:00
abstract::The Bacillus subtilis sacY and sacT genes encode antiterminator proteins, similar to the Escherichia coli bglG gene product and required for transcription of sucrose metabolism genes. A Tn10 insertion into bglP (formerly sytA) has been previously identified as restoring sucrose utilization to a strain with deletions o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1527-1535.1995
更新日期:1995-03-01 00:00:00
abstract::The DNA-dependent RNA polymerase (EC 2.7.7.6) from Clostridium acetobutylicum DSM 1731 has been purified to homogeneity and characterized. The purified enzyme was composed of four subunits and had a molecular mass of 370,000 Da. Western immunoblot analysis with polyclonal antibodies against the sigma 70 subunit of Esc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.2120-2124.1991
更新日期:1991-03-01 00:00:00
abstract::Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.690-693.1979
更新日期:1979-08-01 00:00:00
abstract::We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.24.8094-8101.1992
更新日期:1992-12-01 00:00:00
abstract::Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, a quinolone related to nalidixic acid. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.5.2385-2387.1988
更新日期:1988-05-01 00:00:00
abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02169-12
更新日期:2013-07-01 00:00:00
abstract::The blp locus of a type 6A strain of Streptococcus pneumoniae encodes a two-peptide bacteriocin, pneumocin MN, which mediates intraspecies competition during mouse nasopharyngeal colonization. This locus is regulated by a quorum-sensing mechanism consisting of a dedicated two-component regulatory system and a peptide ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01213-08
更新日期:2009-03-01 00:00:00
abstract::The fumarate reductase enzyme complex allows Escherichia coli to grow anaerobically with fumarate as a terminal electron acceptor for oxidative phosphorylation when the preferred compounds oxygen and nitrate are not available. We used the pKO promoter test vectors to identify a single promoter for the frdABCD genes wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1100-1109.1985
更新日期:1985-12-01 00:00:00
abstract:UNLABELLED:Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00349-15
更新日期:2015-10-01 00:00:00
abstract::Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1624-1626.1995
更新日期:1995-03-01 00:00:00
abstract::The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.525-530.1990
更新日期:1990-02-01 00:00:00
abstract::Atomic force microscopy was used to measure the thickness of air-dried, collapsed murein sacculi from Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Air-dried sacculi from E. coli had a thickness of 3.0 nm, whereas those from P. aeruginosa were 1.5 nm thick. When rehydrated, the sacculi of both bacteria swelle...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.22.6865-6875.1999
更新日期:1999-11-01 00:00:00
abstract::The photoautotrophic cyanobacterium Anacystis nidulans was used to investigate the membrane transport of branched-chain, neutral amino acids and its dependence on photosynthetic reactions. The uptake of alpha-amino [1-14C]isobutyric acid and L-[1-14C]leucine followed Michaelis, Menten kinetics and resulted in an energ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.229-236.1982
更新日期:1982-07-01 00:00:00
abstract::Aspergillus fumigatus secretes a number of glycosidases into the culture medium when the cells are grown in a mineral salts medium containing guar flour (a galactomannan) as the carbon source. At least some of these glycosidases have been reported to be glycoproteins having N-linked oligosaccharides. In this study, we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.67-75.1984
更新日期:1984-10-01 00:00:00
abstract::Moat, Albert G. (Hahnemann Medical College, Philadelphia, Pa.), Isabel J. Barnes, and Eleanor H. McCurley. Factors affecting the survival of auxotrophs and prototrophs of Saccharomyces cerevisiae in mixed populations. J. Bacteriol. 92:297-301. 1966.-The conditions under which the number of yeast prototrophs, as well a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.297-301.1966
更新日期:1966-08-01 00:00:00
abstract::Catabolic mannopine (MOP) cyclase encoded by certain Agrobacterium Ti and Ri plasmids lactonizes MOP to agropine (AGR). The enzyme, purified to homogeneity from a recombinant clone, has a molecular mass of 45 kDa as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2427-2430.1996
更新日期:1996-04-01 00:00:00
abstract::Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.491-498.1973
更新日期:1973-05-01 00:00:00
abstract::The growth constant and Y (sucrose) (grams of cells per mole of sucrose) for NH(3)-grown cultures of Clostridium pasteurianum were 1.7 times those of N(2)-grown cultures, whereas the rate of sucrose utilized per gram of cells per hour was similar for both conditions. The Y (sucrose) of chemostat cultures grown on limi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.2.346-351.1968
更新日期:1968-08-01 00:00:00
abstract::Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3696-3703.1989
更新日期:1989-07-01 00:00:00
abstract::Caldwell, D. R. (U.S. Department of Agriculture, Beltsville, Md.), D. C. White, M. P. Bryant, and R. N. Doetsch. Specificity of the heme requirement for growth of Bacteroides ruminicola. J. Bacteriol. 90:1645-1654. 1965.-Previous studies suggested that most strains of Bacteroides ruminicola subsp. ruminicola require h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.6.1645-1654.1965
更新日期:1965-12-01 00:00:00
abstract::In Rhizobium meliloti, an aspartate aminotransferase (AspAT) encoded within a 7.3-kb HindIII fragment was previously shown to be required for symbiotic nitrogen fixation and aspartate catabolism (V. K. Rastogi and R.J. Watson, J. Bacteriol. 173:2879-2887, 1991). A gene coding for an aromatic aminotransferase located w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1919-1928.1993
更新日期:1993-04-01 00:00:00
abstract::Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6237-6245.1995
更新日期:1995-11-01 00:00:00