Bacteriocin activity of Streptococcus pneumoniae is controlled by the serine protease HtrA via posttranscriptional regulation.

Abstract:

:The blp locus of a type 6A strain of Streptococcus pneumoniae encodes a two-peptide bacteriocin, pneumocin MN, which mediates intraspecies competition during mouse nasopharyngeal colonization. This locus is regulated by a quorum-sensing mechanism consisting of a dedicated two-component regulatory system and a peptide pheromone. Like most clinical isolates, this type 6A strain can be separated into opaque and transparent colony variants, each playing a different role during pneumococcal infection. In this study, we show that the blp locus is differentially regulated at the posttranscriptional level in pneumococcal opacity variants. Transparent and opaque variants produce equivalent amounts of blpMNPO transcript when stimulated with a synthetic pheromone, but transparent variants have no pneumocin MN-mediated inhibitory activity while opaque variants produce large zones of inhibitory activity. The differential regulation in opacity variants is driven by the two-component regulatory system CiaRH via its regulation of the serine protease HtrA. Transparent mutants deficient in CiaH or HtrA show increased pneumocin MN-mediated inhibition. In addition, these mutants demonstrate alterations in their dose response to a synthetic peptide pheromone, suggesting that HtrA activity impacts pneumocin MN production at the level of signaling. This, in addition to its known effects on competence, suggests that HtrA is a pleiotropic regulator whose protease activity affects several important bacterial pathways. The complex regulation of pneumocins may allow the pneumococcus to reserve the secretion of active peptides for situations where the benefit of their inhibitory activity outweighs the cost of their production.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Dawid S,Sebert ME,Weiser JN

doi

10.1128/JB.01213-08

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

1509-18

issue

5

eissn

0021-9193

issn

1098-5530

pii

JB.01213-08

journal_volume

191

pub_type

杂志文章
  • Identification of genes encoding exported Mycobacterium tuberculosis proteins using a Tn552'phoA in vitro transposition system.

    abstract::Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.10.2732-2740.2000

    authors: Braunstein M,Griffin TJ IV,Kriakov JI,Friedman ST,Grindley ND,Jacobs WR Jr

    更新日期:2000-05-01 00:00:00

  • Methylation Warfare: Interaction of Pneumococcal Bacteriophages with Their Host.

    abstract::Virus-host interactions are regulated by complex coevolutionary dynamics. In Streptococcus pneumoniae, phase-variable type I restriction-modification (R-M) systems are part of the core genome. We hypothesized that the ability of the R-M systems to switch between six target DNA specificities also has a key role in prev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00370-19

    authors: Furi L,Crawford LA,Rangel-Pineros G,Manso AS,De Ste Croix M,Haigh RD,Kwun MJ,Engelsen Fjelland K,Gilfillan GD,Bentley SD,Croucher NJ,Clokie MR,Oggioni MR

    更新日期:2019-09-06 00:00:00

  • Characteristics of penicillinase secretion by growing cells and protoplasts of Bacillus licheniformis.

    abstract::Cultures of the inducible penicillinase-producing strain 749 of Bacillus licheniformis, induced with small amounts of benzylpenicillin, synthesized penicillinase at a high rate for a short period, after which the rate of synthesis slowly declined. During the period of active synthesis, the rate of secretion, as a frac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.820-826.1969

    authors: Sargent MG,Ghosh BK,Lampen JO

    更新日期:1969-02-01 00:00:00

  • The a locus governs cytoduction in Ustilago maydis.

    abstract::We have developed a cytoduction assay to measure cell fusion quantitatively in the basidiomycete corn smut fungus Ustilago maydis. This assay employs a mutation conferring resistance to oligomycin that exhibits non-Mendelian inheritance and presumably affects the mitochondrial genome. After auxotrophic olir cells are ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.23.7831-7833.1992

    authors: Trueheart J,Herskowitz I

    更新日期:1992-12-01 00:00:00

  • Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.

    abstract::Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.479-489.1979

    authors: Linn T,Goman M,Scaife J

    更新日期:1979-11-01 00:00:00

  • Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents.

    abstract::Cook, Thomas M. (Sterling-Winthrop Research Institute, Rensselaer, N.Y.), William H. Deitz, and William A. Goss. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J. Bacteriol. 91:774-779. 1996.-Treatment of Escherichia coli 15TAU with nalidixic acid resu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.774-779.1966

    authors: Cook TM,Deitz WH,Goss WA

    更新日期:1966-02-01 00:00:00

  • Distance between alleles as a determinant of linkage in natural transformation of Acinetobacter calcoaceticus.

    abstract::Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.20.6015-6017.1995

    authors: Kloos DU,DiMarco AA,Elsemore DA,Timmis KN,Ornston LN

    更新日期:1995-10-01 00:00:00

  • Depletion of glycolytic intermediates plays a key role in glucose-phosphate stress in Escherichia coli.

    abstract::In bacteria like Escherichia coli, the accumulation of glucose-6-phosphate (G6P) or its analogs such as α-methyl glucoside-6-phosphate (αMG6P) results in stress that appears in the form of growth inhibition. The small RNA SgrS is an essential part of the response that helps E. coli combat glucose-phosphate stress; the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00705-13

    authors: Richards GR,Patel MV,Lloyd CR,Vanderpool CK

    更新日期:2013-11-01 00:00:00

  • Developmental control of glucosamine and galactosamine levels during conidation in Neurospora crassa.

    abstract::The glucosamine and galactosamine content of mycelia was measured in cultures of Neurospora crassa grown on the surface of dialysis membranes. The glucosamine content was relatively constant throughout the different regions of the mycelial mat. The galactosamine content, however, was always lower in the growing-front ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.1071-1075.1975

    authors: Schmit JC,Brody S

    更新日期:1975-06-01 00:00:00

  • Roles for the Rhodobacter sphaeroides CcmA and CcmG proteins.

    abstract::Rhodobacter sphaeroides cells containing an in-frame deletion within ccmA lack detectable soluble and membrane-bound c-type cytochromes and are unable to grow under conditions where these proteins are required. Only strains merodiploid for ccmABCDG were found after attempting to generate cells containing either a ccmG...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.15.4643-4647.2001

    authors: Cox RL,Patterson C,Donohue TJ

    更新日期:2001-08-01 00:00:00

  • The Enterococcus faecalis sigV protein is an extracytoplasmic function sigma factor contributing to survival following heat, acid, and ethanol treatments.

    abstract::Analysis of the genome sequence of Enterococcus faecalis allowed the identification of two genes whose protein products showed 33 and 34% identity with those of sigV and yrhM of Bacillus subtilis, respectively. These genes, named sigV and rsiV, are predicted to encode members of the extracytoplasmic function subfamily...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.3.1022-1035.2005

    authors: Benachour A,Muller C,Dabrowski-Coton M,Le Breton Y,Giard JC,Rincé A,Auffray Y,Hartke A

    更新日期:2005-02-01 00:00:00

  • Changes in Escherichia coli rRNA promoter activity correlate with changes in initiating nucleoside triphosphate and guanosine 5' diphosphate 3'-diphosphate concentrations after induction of feedback control of ribosome synthesis.

    abstract::rRNA synthesis is the rate-limiting step in ribosome synthesis in Escherichia coli. Its regulation has been described in terms of a negative-feedback control loop in which rRNA promoter activity responds to the amount of translation. The feedback nature of this control system was demonstrated previously by artificiall...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.20.6185-6191.2003

    authors: Schneider DA,Gourse RL

    更新日期:2003-10-01 00:00:00

  • Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.

    abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.125-135.1976

    authors: Guild WR,Shoemaker NB

    更新日期:1976-01-01 00:00:00

  • EVIDENCE OF LYSOGENY IN DERIVATIVES OF ESCHERICHIA COLI.

    abstract::Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.446-452.1965

    authors: FRAMPTON EW,BRINKLEY BR

    更新日期:1965-08-01 00:00:00

  • Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.

    abstract::The ski22::Tn917lac insertion mutation in Bacillus subtilis was isolated in a screen for mutations that cause a defect in sporulation but are suppressed by the presence or overexpression of the histidine protein kinase encoded by kinA (spoIIJ). The ski22::Tn917lac insertion mutation was in ald, the gene encoding alani...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.21.6789-6796.1993

    authors: Siranosian KJ,Ireton K,Grossman AD

    更新日期:1993-11-01 00:00:00

  • Functional Characterization of a Novel Member of the Amidohydrolase 2 Protein Family, 2-Hydroxy-1-Naphthoic Acid Nonoxidative Decarboxylase from Burkholderia sp. Strain BC1.

    abstract:UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00250-16

    authors: Pal Chowdhury P,Basu S,Dutta A,Dutta TK

    更新日期:2016-05-27 00:00:00

  • Life cycle of Neurospora crassa viewed by scanning electron microscopy.

    abstract::Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.1a and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and ou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.1015-1025.1973

    authors: Seale T

    更新日期:1973-02-01 00:00:00

  • Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39.

    abstract::We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01204-09

    authors: Tsui HC,Mukherjee D,Ray VA,Sham LT,Feig AL,Winkler ME

    更新日期:2010-01-01 00:00:00

  • Characterization of glucose-specific catabolite repression-resistant mutants of Bacillus subtilis: identification of a novel hexose:H+ symporter.

    abstract::Insertional mutagenesis was conducted on Bacillus subtilis cells to screen for mutants resistant to catabolite repression. Three classes of mutants that were resistant to glucose-promoted but not mannitol-promoted catabolite repression were identified. Cloning and sequencing of the mutated genes revealed that the muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.3.498-504.1998

    authors: Paulsen IT,Chauvaux S,Choi P,Saier MH Jr

    更新日期:1998-02-01 00:00:00

  • Coordination chemistry of microbial iron transport compounds: rhodotorulic acid and iron uptake in Rhodotorula pilimanae.

    abstract::The mechanism by which iron uptake is facilitated by the siderophore rhodotorulic acid (RA) in the yeast Rhodotorula pilimanae was investigated with radioactively labeled Fe and RA and kinetically inert, chromic-substituted RA complexes. The weight of the evidence supports a model in which RA mediates iron transport t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.69-74.1978

    authors: Carrano CJ,Raymond KN

    更新日期:1978-10-01 00:00:00

  • Tyrosine phosphorylation of a membrane protein from Pseudomonas solanacearum.

    abstract::We have investigated a tyrosine kinase activity from Pseudomonas solanacearum, an economically important plant pathogen. In vitro incubation of membrane fractions with [gamma-32P]ATP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an 85-kDa phosphoprotein. Phosphorylation of this prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.13.4356-4360.1992

    authors: Atkinson M,Allen C,Sequeira L

    更新日期:1992-07-01 00:00:00

  • Regulation of Bacillus subtilis sigmaH (spo0H) and AbrB in response to changes in external pH.

    abstract::The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.21.6778-6787.1997

    authors: Cosby WM,Zuber P

    更新日期:1997-11-01 00:00:00

  • Promoter of the pertussis toxin operon and production of pertussis toxin.

    abstract::Pertussis toxin (PT), the major virulence factor of Bordetella pertussis, is composed of five different subunits whose genes are organized as an operon. We report the mapping of the promoter region of the PT operon and show that this promoter is only weakly active in Escherichia coli. Bordetella parapertussis and Bord...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2843-2846.1987

    authors: Nicosia A,Rappuoli R

    更新日期:1987-06-01 00:00:00

  • Factors affecting cellulolysis by Ruminococcus albus.

    abstract::The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.729-737.1973

    authors: Smith WR,Yu I,Hungate RE

    更新日期:1973-05-01 00:00:00

  • Transfection of group H streptococci.

    abstract::Transfection of streptococci is reported here for the first time. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1505-1506.1973

    authors: Parsons CL,Cole RM

    更新日期:1973-03-01 00:00:00

  • Purification and N-terminal sequence of the alpha subunit of antigen 43, a unique protein complex associated with the outer membrane of Escherichia coli.

    abstract::Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3634-3640.1989

    authors: Caffrey P,Owen P

    更新日期:1989-07-01 00:00:00

  • Analysis of dofA, a fruA-dependent developmental gene, and its homologue, dofB, in Myxococcus xanthus.

    abstract::The developmentally regulated gene dofA, identified from pulse-labeling experiments by two-dimensional gel electrophoresis, and its homologue, dofB, were cloned and characterized in Myxococcus xanthus. Deletion of dofA and dofB did not affect the vegetative growth and development of M. xanthus. dofA was specifically e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.24.6803-6810.2002

    authors: Horiuchi T,Akiyama T,Inouye S,Komano T

    更新日期:2002-12-01 00:00:00

  • Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures.

    abstract::Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.4.1240-1247.1992

    authors: Delaney JM,Ang D,Georgopoulos C

    更新日期:1992-02-01 00:00:00

  • Genetic rearrangement associated with in vivo mucoid conversion of Pseudomonas aeruginosa PAO is due to insertion elements.

    abstract::The conversion of Pseudomonas aeruginosa PAO to the mucoid phenotype has been reported for a chronic pulmonary infection model in rats (D. E. Woods, P. A. Sokol, L. E. Bryan, D. G. Storey, S. J. Mattingly, H. J. Vogel, and H. Ceri, J. Infect. Dis. 163:143-149, 1991). This conversion was associated with a genetic rearr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.3.553-562.1994

    authors: Sokol PA,Luan MZ,Storey DG,Thirukkumaran P

    更新日期:1994-02-01 00:00:00

  • Overproduction of the AlgT Sigma Factor Is Lethal to Mucoid Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa isolates from chronic lung infections often overproduce alginate, giving rise to the mucoid phenotype. Isolation of mucoid strains from chronic lung infections correlates with a poor patient outcome. The most common mutation that causes the mucoid phenotype is called mucA22 and results in a trun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00445-20

    authors: Cross AR,Raghuram V,Wang Z,Dey D,Goldberg JB

    更新日期:2020-09-23 00:00:00