Regulation of Salmonella enterica serovar Typhimurium mntH transcription by H(2)O(2), Fe(2+), and Mn(2+).


:MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Consistent with its phenotype and function, mntH is regulated at the transcriptional level by both H(2)O(2) and substrate cation. We have now identified three trans-acting regulatory factors and the three corresponding cis-acting mntH promoter motifs that mediate this regulation. In the presence of hydrogen peroxide, mntH is activated by OxyR, acting through an OxyR-binding motif centered just upstream of the likely -35 RNA polymerase-binding site. In the presence of Fe(2+), mntH is repressed primarily by Fur, acting through a Fur-binding motif overlapping the -35 region. In the presence of Mn(2+), mntH is repressed primarily by the Salmonella equivalent of E. coli b0817, a distant homolog of the Bacillus subtilis manganese transport repressor, MntR, acting through an inverted-repeat motif located between the likely -10 polymerase binding site and the ribosome binding site. E. coli b0817 was recently shown to bind the identical inverted-repeat motif in the E. coli mntH promoter and hence has been renamed MntR (S. I. Patzer and K. Hantke, J. Bacteriol. 183:4806-4813, 2001). Using Deltafur, DeltamntR, and Deltafur DeltamntR mutant strains as well as mutations in the Fur- and MntR-binding motif elements, we found that Fe(2+) can also mediate repression through the Mn(2+) repressor MntR.


J Bacteriol


Journal of bacteriology


Kehres DG,Janakiraman A,Slauch JM,Maguire ME





Has Abstract


2002-06-01 00:00:00












  • Expression of a mutation affecting F incompatibility in the integrated but not the autonomous state of F.

    abstract::Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pfister A,DeVries JK,Maas WK

    更新日期:1976-07-01 00:00:00

  • Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes.

    abstract::A mutational analysis of lesion-forming ability was undertaken in Pseudomonas syringae pv. syringae B728a, causal agent of bacterial brown spot disease of bean. Following a screen of 6,401 Tn5-containing derivatives of B728a on bean pods, 26 strains that did not form disease lesions were identified. Nine of the mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rich JJ,Willis DK

    更新日期:1997-04-01 00:00:00

  • An endo-N-acetyl-beta-D-glucosaminidase, acting on the di-N-acetylchitobiosyl part of N-linked glycans, is secreted during sporulation of Myxococcus xanthus.

    abstract::After the demonstration that Stigmatella aurantiaca DW4 secretes an endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans (S. Bourgerie, Y. Karamanos, T. Grard, and R. Julien, J. Bacteriol. 176:6170-6174, 1994), an ENGase activity having the same substrate specifi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Barreaud JP,Bourgerie S,Julien R,Guespin-Michel JF,Karamanos Y

    更新日期:1995-02-01 00:00:00

  • The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene.

    abstract::Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang L,Huskic S,Cisterne A,Rothemund D,Reeves PR

    更新日期:2002-05-01 00:00:00

  • Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).

    abstract::The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bedford DJ,Schweizer E,Hopwood DA,Khosla C

    更新日期:1995-08-01 00:00:00

  • Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli.

    abstract::The pattern of transcription of the rplKAJLrpoBC gene cluster of Escherichia coli appears to be complex. At least four different promoters and a transcriptional attenuator have been identified. To compare the relative effect of each of the putative promoters and the attenuator on transcription of these genes, we fused...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ralling G,Linn T

    更新日期:1984-04-01 00:00:00

  • Isolation and characterization of Tn917lac-generated competence mutants of Bacillus subtilis.

    abstract::We isolated 28 mutants of Bacillus subtilis deficient in the development of competence by using the transposon Tn917lacZ as a mutagen. The mutant strains were poorly transformable with plasmid and chromosomal DNAs but were normally transducible and exhibited wild-type resistance to DNA-damaging agents. The mutations w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hahn J,Albano M,Dubnau D

    更新日期:1987-07-01 00:00:00

  • Mapping and disruption of the chpB locus in Escherichia coli.

    abstract::The chpB locus is a chromosomal homolog of the pem locus, which is responsible for stable maintenance of plasmid R100 within the host cells. Like pem, chpB codes for two genes, chpBK and chpBI, encoding a growth inhibitor and a suppressor for the killing action of the ChpBK protein, respectively. Here, we determined t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Masuda Y,Ohtsubo E

    更新日期:1994-09-01 00:00:00

  • A LysR-type regulator, CidR, is required for induction of the Staphylococcus aureus cidABC operon.

    abstract::The Staphylococcus aureus cidABC and lrgAB operons have been shown to regulate murein hydrolase activity and affect antibiotic tolerance. The cid operon enhances murein hydrolase activity and antibiotic sensitivity, whereas the lrg operon inhibits these processes. Based on these findings and the structural similaritie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yang SJ,Rice KC,Brown RJ,Patton TG,Liou LE,Park YH,Bayles KW

    更新日期:2005-09-01 00:00:00

  • Protoplast formation in Escherichia coli.

    abstract::A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Weiss RL

    更新日期:1976-11-01 00:00:00

  • Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein.

    abstract::Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Southworth MW,Perler FB

    更新日期:2002-11-01 00:00:00

  • The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.

    abstract::In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: De Wulf P,Kwon O,Lin EC

    更新日期:1999-11-01 00:00:00

  • Macroconidial germination in Microsporum gypseum.

    abstract::Biochemical events which occur during macroconidial germination have been studied in the dermatophyte Microsporum gypseum. The specific activity levels of various metabolic enzymes have been assayed during germination time periods. The accumulated levels of several of these enzymes, as a function of exogenous carbohyd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leighton TJ,Stock JJ,Kelln RA

    更新日期:1970-08-01 00:00:00


    abstract::Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-12-01 00:00:00

  • Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli.

    abstract::The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Moralejo P,Egan SM,Hidalgo E,Aguilar J

    更新日期:1993-09-01 00:00:00

  • Synergistic effect of himA and gyrB mutations: evidence that him functions control expression of ilv and xyl genes.

    abstract::We have constructed Escherichia coli strains containing mutations at two different loci, both originally selected for failure to support lambda site-specific recombination: himA and gyrB-him(Ts). Although the gyrB-him(Ts) mutations by themselves reduce supercoiling at high temperature, the double mutants show a far gr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Friedman DI,Olson EJ,Carver D,Gellert M

    更新日期:1984-02-01 00:00:00

  • Distribution of an L-isoaspartyl protein methyltransferase in eubacteria.

    abstract::A protein carboxyl methyltransferase (EC that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. This enzyme catalyzes the transfer of methyl groups from S-adenosylmethionine to the carboxyl groups of D-aspartyl o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Li C,Clarke S

    更新日期:1992-01-01 00:00:00

  • Flexible Hinges in Bacterial Chemoreceptors.

    abstract::Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted recepto...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Akkaladevi N,Bunyak F,Stalla D,White TA,Hazelbauer GL

    更新日期:2018-02-07 00:00:00

  • Biosynthesis of branched-chain amino acids in Schizosaccharomyces pombe: properties of acetohydroxy acid synthetase.

    abstract::The regulatory properties of acetohydroxy acid synthetase (AHAS), the first enzyme in the biosynthetic pathway to valine and the second in the isoleucine pathway, were investigated in the fission yeast Schizosaccharomyces pombe. The enzyme was partially purified from crude extracts by protamine sulfate treatment, ammo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McDonald RA,Satyanarayana T,Kaplan JG

    更新日期:1973-04-01 00:00:00

  • Inducible gamma-butyrobetaine-degrading enzymes in Pseudomonas species AK 1.

    abstract::Activities of gamma-butyrobetaine hydroxylase and carnitine dehydrogenase were low in cells of Pseudomonas sp. AK 1 grown in the absence of their respective substrates. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lindstedt G,Lindstedt S,Midtvedt T,Tofft M

    更新日期:1970-03-01 00:00:00

  • Electron probe analysis, X-ray mapping, and electron energy-loss spectroscopy of calcium, magnesium, and monovalent ions in log-phase and in dividing Escherichia coli B cells.

    abstract::The elemental composition of individual cells of rapidly frozen and cryosectioned Escherichia coli B was measured with electron optical microanalytic methods. The Ca content was high (26.2 mmol/kg) in a 10-nm-wide region of the cell envelope. Amounts of cytoplasmic Ca in actively dividing cells were significantly high...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chang CF,Shuman H,Somlyo AP

    更新日期:1986-09-01 00:00:00

  • Polymorphisms in inc proteins and differential expression of inc genes among Chlamydia trachomatis strains correlate with invasiveness and tropism of lymphogranuloma venereum isolates.

    abstract::Chlamydia trachomatis is a human bacterial pathogen that multiplies only within an intracellular membrane-bound vacuole, the inclusion. C. trachomatis includes ocular and urogenital strains, usually causing infections restricted to epithelial cells of the conjunctiva and genital mucosa, respectively, and lymphogranulo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Almeida F,Borges V,Ferreira R,Borrego MJ,Gomes JP,Mota LJ

    更新日期:2012-12-01 00:00:00

  • Instability of pathogenicity islands in uropathogenic Escherichia coli 536.

    abstract::The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled al...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Middendorf B,Hochhut B,Leipold K,Dobrindt U,Blum-Oehler G,Hacker J

    更新日期:2004-05-01 00:00:00

  • Definition of a minimal plasmid stabilization system from the broad-host-range plasmid RK2.

    abstract::The stable inheritance of the broad-host-range plasmid RK2 is due at least in part to functions within a region located at coordinates 32.8 to 35.9 kb, termed the RK2 par locus. This locus encodes four previously identified genes in two operons (parCBA and parD; M. Gerlitz, O. Hrabak, and H. Schwab, J. Bacteriol. 172:...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Roberts RC,Helinski DR

    更新日期:1992-12-01 00:00:00

  • Identification of the lipopolysaccharide core region as the receptor site for a cytotoxin-converting phage, phi CTX, of Pseudomonas aeruginosa.

    abstract::A temperate phage, phi CTX, is a cytotoxin-converting phage of Pseudomonas aeruginosa. In this study, we characterized the lipopolysaccharide (LPS) structures of phi CTX-resistant mutants derived from phi CTX-sensitive strains. phi CTX infectivity was neutralized by LPS preparations derived from sensitive strains but ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yokota S,Hayashi T,Matsumoto H

    更新日期:1994-09-01 00:00:00

  • Phosphatidylglycerolphosphate synthase expression in Schizosaccharomyces pombe is regulated by the phospholipid precursors inositol and choline.

    abstract::The enzyme phosphatidylglycerolphosphate synthase (PGPS; CDP-diacylglycerol glycerol 3-phosphate 3-phosphatidyltransferase; EC catalyzes the committed step in the cardiolipin biosynthetic pathway. To study the regulation of PGPS in Schizosaccharomyces pombe, we characterized the enzyme biochemically. Maximum ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Karkhoff-Schweizer RR,Kelly BL,Greenberg ML

    更新日期:1991-10-01 00:00:00

  • Mutations in ampG or ampD affect peptidoglycan fragment release from Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Garcia DL,Dillard JP

    更新日期:2008-06-01 00:00:00

  • Antibodies Damage the Resilience of Fimbriae, Causing Them To Be Stiff and Tangled.

    abstract::As adhesion fimbriae are a major virulence factor for many pathogenic Gram-negative bacteria, they are also potential targets for antibodies. Fimbriae are commonly required for initiating the colonization that leads to disease, and their success as adhesion organelles lies in their ability to both initiate and sustain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Singh B,Mortezaei N,Savarino SJ,Uhlin BE,Bullitt E,Andersson M

    更新日期:2016-12-13 00:00:00

  • Streptococcus salivarius fimbriae are composed of a glycoprotein containing a repeated motif assembled into a filamentous nondissociable structure.

    abstract::Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lévesque C,Vadeboncoeur C,Chandad F,Frenette M

    更新日期:2001-05-01 00:00:00

  • SodA Contributes to the Virulence of Avian Pathogenic Escherichia coli O2 Strain E058 in Experimentally Infected Chickens.

    abstract::Strains of avian pathogenic Escherichia coli (APEC), the common pathogen of avian colibacillosis, encounter reactive oxygen species (ROS) during the infection process. Superoxide dismutases (SODs), acting as antioxidant factors, can protect against ROS-mediated host defenses. Our previous reports showed that the sodA ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gao Q,Xia L,Wang X,Ye Z,Liu J,Gao S

    更新日期:2019-02-25 00:00:00