Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli.

Abstract:

:Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enzymes, that oligoribonuclease is not a manifestation of RNases D, BN, T, PH, and R, exoribonucleases discovered subsequently. Thus, oligoribonuclease is the eighth distinct exoribonuclease discovered in Escherichia coli. We also show that oligoribonuclease copurifies with polynucleotide phosphorylase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yu D,Deutscher MP

doi

10.1128/jb.177.14.4137-4139.1995

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

4137-9

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

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