Vibrio cholerae H-NS silences virulence gene expression at multiple steps in the ToxR regulatory cascade.

Abstract:

:H-NS is an abundant nucleoid-associated protein involved in the maintenance of chromosomal architecture in bacteria. H-NS also has a role in silencing the expression of a variety of environmentally regulated genes during growth under nonpermissive conditions. In this study we demonstrate a role for H-NS in the negative modulation of expression of several genes within the ToxR virulence regulon of Vibrio cholerae. Deletion of hns resulted in high, nearly constitutive levels of expression of the genes encoding cholera toxin, toxin-coregulated pilus, and the ToxT virulence gene regulatory protein. For the cholera toxin- and ToxT-encoding genes, elevated expression in an hns mutant was found to occur in the absence of the cognate activator proteins, suggesting that H-NS functions directly at these promoters to decrease gene expression. Deletion analysis of the region upstream of toxT suggests that an extensive region located far upstream of the transcriptional start site is required for complete H-NS-mediated repression of gene expression. These data indicate that H-NS negatively influences multiple levels of gene expression within the V. cholerae virulence cascade and raise the possibility that the transcriptional activator proteins in the ToxR regulon function to counteract the repressive effects of H-NS at the various promoters as well as to recruit RNA polymerase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Nye MB,Pfau JD,Skorupski K,Taylor RK

doi

10.1128/jb.182.15.4295-4303.2000

keywords:

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

4295-303

issue

15

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

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