In situ analysis of sulfur species in sulfur globules produced from thiosulfate by Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes.

abstract：:Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance tha...

journal_title：Journal of bacteriology

authors： Hoffmann HP,Geftic SG,Gelzer J,Heymann H,Adair FW

更新日期：1973-01-01 00:00:00

abstract：:Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...

journal_title：Journal of bacteriology

authors： Serrano M,Vieira F,Moran CP Jr,Henriques AO

更新日期：2008-12-01 00:00:00

abstract：:soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...

journal_title：Journal of bacteriology

authors： Wu J,Weiss B

更新日期：1991-05-01 00:00:00

abstract：:Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

journal_title：Journal of bacteriology

authors： Guo G,Ding Y,Weiss B

更新日期：1997-01-01 00:00:00

abstract：:Strain IMCC9480 is a novel member of the family Oxalobacteraceae of the Betaproteobacteria, isolated from the Arctic Ocean by dilution-to-extinction culturing. Here we present the draft genome sequence of strain IMCC9480. The genome is predicted to contain genes for xanthorhodopsin, retinoid biosynthesis, carbon monox...

journal_title：Journal of bacteriology

authors： Oh HM,Lee K,Jang Y,Kang I,Kim HJ,Kang TW,Kim SY,Cho JC

更新日期：2011-07-01 00:00:00

abstract：:After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...

journal_title：Journal of bacteriology

authors： Terry KR,Hooper AB

更新日期：1970-07-01 00:00:00

abstract：:Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number...

journal_title：Journal of bacteriology

authors： Sun LV,Foster JM,Tzertzinis G,Ono M,Bandi C,Slatko BE,O'Neill SL

更新日期：2001-04-01 00:00:00

abstract：:Four regulated promoters that direct the transcription of genes (i.e., korA, tra, kilB, and korB) involved in the transfer of the Streptomyces plasmid pIJ101 were isolated following the in vitro fusion of plasmid DNA fragments to a promoterless gene encoding the S. lividans extracellular enzyme beta-galactosidase. Int...

journal_title：Journal of bacteriology

authors： Stein DS,Kendall KJ,Cohen SN

更新日期：1989-11-01 00:00:00

abstract：:Globomycin is an antibiotic which inhibits the processing of the prolipoprotein. Eighty globomycin-resistant mutants were independently isolated from Escherichia coli K-12 which had a deletion mutation in chromosomal lipoprotein gene (lpp), but contained a plasmid carrying the wild-type lpp gene. Twenty-six of the mut...

journal_title：Journal of bacteriology

authors： Zwiebel LJ,Inukai M,Nakamura K,Inouye M

更新日期：1981-01-01 00:00:00

abstract：:A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...

journal_title：Journal of bacteriology

authors： Maniloff J,Quinlan DC

更新日期：1974-10-01 00:00:00

abstract：:The SecYEG heterotrimeric membrane protein complex functions as a channel for protein translocation across the Escherichia coli cytoplasmic membrane. SecY is the central subunit of the SecYEG complex and contains 10 transmembrane segments (TM1 to TM10). Previous mutation studies suggested that TM3 and TM4 are particul...

journal_title：Journal of bacteriology

authors： Mori H,Shimokawa N,Satoh Y,Ito K

更新日期：2004-06-01 00:00:00

abstract：:Rhythmically advancing waves of cells, called ripples, arise spontaneously during the aggregation of Myxococcus xanthus into fruiting bodies. Extracts prepared by washing rippling cells contain a substance that will induce quiescent cells to ripple. Three lines of evidence indicate that murein (peptidoglycan) is the r...

journal_title：Journal of bacteriology

authors： Shimkets LJ,Kaiser D

更新日期：1982-10-01 00:00:00

abstract：:The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...

journal_title：Journal of bacteriology

authors： Brinkman FS,Schoofs G,Hancock RE,De Mot R

更新日期：1999-08-01 00:00:00

abstract：:d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...

journal_title：Journal of bacteriology

authors： Boulter JR,Gielow WO

更新日期：1973-02-01 00:00:00

abstract：:Antiserum to Aeromonas hydrophila A6 cell envelopes was shown in a previous study (C. Y. F. Wong, G. Mayrhofer, M. W. Heuzenroeder, H. M. Atkinson, D. M. Quinn, and R. L. P. Flower, FEMS Immunol. Med. Microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. In this study, colony blot ana...

journal_title：Journal of bacteriology

authors： Wong CY,Heuzenroeder MW,Quinn DM,Flower RL

更新日期：1997-06-01 00:00:00

abstract：:Exogenously supplied long-chain fatty acids have been shown to markedly alleviate the inhibition of phototrophic growth of cultures of Rhodopseudomonas sphaeroides caused by the antibiotic cerulenin. Monounsaturated and polyunsaturated C18 fatty acids were most effective in relieving growth inhibition mediated by ceru...

journal_title：Journal of bacteriology

authors： Campbell TB,Lueking DR

更新日期：1983-02-01 00:00:00

abstract：:The hemA mutation of Escherichia coli K-12 confers a requirement for delta-aminolevulinic acid (ALA). Cell extract prepared from the hemA strain SASX41B was incapable of producing ALA from either glutamate or glutamyl-tRNA, whereas extract of the hem+ strain HB101 formed colorimetrically detectable amounts of ALA and ...

journal_title：Journal of bacteriology

authors： Avissar YJ,Beale SI

更新日期：1989-06-01 00:00:00

abstract：:Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...

journal_title：Journal of bacteriology

authors： Baron SF,Franklund CV,Hylemon PB

更新日期：1991-08-01 00:00:00

abstract：:Wright, D. N. (Iowa State University, Ames), and W. R. Lockhart. Environmental control of cell composition in Escherichia coli. J. Bacteriol. 89:1026-1031. 1965.-Escherichia coli was grown in continuous culture at various rates in a defined medium with either the carbon or the nitrogen source as rate-limiting substrat...

journal_title：Journal of bacteriology

authors： WRIGHT DN,LOCKHART WR

更新日期：1965-04-01 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...

journal_title：Journal of bacteriology

authors： Smith CJ,Spiegel H

更新日期：1987-08-01 00:00:00

abstract：:In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...

journal_title：Journal of bacteriology

authors： Ballesta JP,De Garcia CL,Schaechter M

更新日期：1973-10-01 00:00:00

abstract：:The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-s...

journal_title：Journal of bacteriology

authors： Tong J,Wetmur JG

更新日期：1996-05-01 00:00:00

abstract：:Martin, Alice J. (Nassau County Department of Health, Hempstead, N.Y.), and Margaret O'Brien. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bacteriol. 89:570-573. 1965.-The application of the fluorescent-antibody (FA) technique to cultures of f...

journal_title：Journal of bacteriology

authors： MARTIN AJ,O'BRIEN M

更新日期：1965-03-01 00:00:00

abstract：:The copy number and stability of artificial 2-micron circle-based plasmids have been accurately measured in [Cir+] and [Cir0] strains of Saccharomyces cerevisiae. We conclude that (i) instability and copy number vary greatly from plasmid to plasmid; (ii) instability and copy number are negatively correlated--that is, ...

journal_title：Journal of bacteriology

authors： Futcher AB,Cox BS

更新日期：1984-01-01 00:00:00

abstract：:Yeast cells, Candida utilis, in water suspension and in the absence of electrolytes were found to be very sensitive to several proteins of moderate size, including ribonuclease, protamine, lysozyme, bovine serum albumin, cytochrome c, and myoglobin. Viability ceases rapidly, and ultraviolet-absorbing compounds (260 mm...

journal_title：Journal of bacteriology

authors： Yphantis DA,Dainko JL,Schlenk F

更新日期：1967-11-01 00:00:00

abstract：:In Listeria monocytogenes, the full details of how stress signals are integrated into the σB regulatory pathway are not yet available. To help shed light on this question, we investigated a collection of transposon mutants that were predicted to have compromised activity of the alternative sigma factor B (σB). These m...

journal_title：Journal of bacteriology

authors： Guerreiro DN,Wu J,Dessaux C,Oliveira AH,Tiensuu T,Gudynaite D,Marinho CM,Boyd A,García-Del Portillo F,Johansson J,O'Byrne CP

更新日期：2020-04-09 00:00:00

abstract：:We demonstrate that the expression of hem genes in Rhodobacter capsulatus is transcriptionally repressed in response to the exogenous addition of heme. A high-copy suppressor screen for regulators of hem gene expression resulted in the identification of an LysR-type transcriptional regulator, called HbrL, that regulat...

journal_title：Journal of bacteriology

authors： Smart JL,Bauer CE

更新日期：2006-02-01 00:00:00

abstract：:The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...

journal_title：Journal of bacteriology

authors： Wu CY,Blaszczak LC,Smith MC,Skatrud PL

更新日期：1994-03-01 00:00:00

abstract：:Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...

journal_title：Journal of bacteriology

authors： Kovacs SA,O'Neil J,Watcharapijarn J,Moe-Kirvan C,Vijay S,Silva V

更新日期：1993-04-01 00:00:00