Abstract:
:The small regulatory protein Crl activates sigma(S) (RpoS), the stationary-phase and general stress response sigma factor. Crl has been reported to bind sigma(S) in vitro and to facilitate the formation of RNA polymerase holoenzyme. In Salmonella enterica serovar Typhimurium, Crl is required for the development of the rdar morphotype and transcription initiation of the sigma(S)-dependent genes csgD and adrA, involved in curli and cellulose production. Here, we examined the expression of other sigma(S)-dependent phenotypes and genes in a Deltacrl mutant of Salmonella. Gene fusion analyses and in vitro transcription assays indicate that the magnitude of Crl activation differs between promoters and is highly dependent on sigma(S) levels. We replaced the wild-type rpoS allele in S. enterica serovar Typhimurium strain ATCC 14028 with the rpoS(LT2) allele that shows reduced expression of sigma(S); the result was an increased Crl activation ratio and larger physiological effects of Crl on oxidative, thermal, and acid stress resistance levels during stationary phase. We also found that crl, rpoS, and crl rpoS strains grew better on succinate than did the wild type and expressed the succinate dehydrogenase sdhCDBA operon more strongly. The crl and rpoS(LT2) mutations also increased the competitive fitness of Salmonella in stationary phase. These results show that Crl contributes to negative regulation by sigma(S), a finding consistent with a role for Crl in sigma factor competition via the facilitation of sigma(S) binding to core RNA polymerase.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Robbe-Saule V,Lopes MD,Kolb A,Norel Fdoi
10.1128/JB.01919-06subject
Has Abstractpub_date
2007-04-01 00:00:00pages
2976-87issue
8eissn
0021-9193issn
1098-5530pii
JB.01919-06journal_volume
189pub_type
杂志文章abstract::A spontaneously arising regulatory mutant of the gluconate system in Escherichia coli was isolated. This mutant became constitutive, probably in one step, for gluconate high-affinity transport, gluconokinase, and gluconate-6-P dehydrase. The mutation involved (gntR18) is cotransducible with asd. Pseudorevertants, deri...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.469-473.1973
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abstract::We investigated an osmotic remedial mutant of Saccharomyces which was deficient in galactose-1-phosphate uridyl transferase. Both the rate of growth and the transferase activity of the mutant culture were dependent on the osmotic activity of the growth medium. Organic and ionic solutes proved to be equally effective i...
journal_title:Journal of bacteriology
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doi:10.1128/JB.95.3.1103-1110.1968
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.20.5867-5876.1996
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.24.6649-6654.1998
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journal_title:Journal of bacteriology
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doi:10.1128/JB.122.3.886-898.1975
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/JB.149.3.1112-1119.1982
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.901-907.1977
更新日期:1977-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1367-1371.1983
更新日期:1983-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1976-03-01 00:00:00