Rapid viability loss on exposure to air in a superoxide dismutase-deficient mutant of Porphyromonas gingivalis.

Abstract:

:Porphyromonas gingivalis, an obligate anaerobe, exhibits a relatively high degree of aerotolerance and possesses superoxide dismutase (SOD) which is induced by exposure to air. To clarify roles for SOD in this organism, the gene encoding SOD (sod) on the P. gingivalis chromosome was disrupted in a gene-directed way by use of a suicide plasmid containing a mutated sod. A sod mutant thus obtained showed no SOD activity in crude extracts and exhibited a rapid viability loss immediately after exposure to air, whereas the wild-type parent showed no decrease in viability for at least 5 h under aerobic conditions. These results clearly indicate that SOD is essential for aerotolerance in P. gingivalis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Nakayama K

doi

10.1128/jb.176.7.1939-1943.1994

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

1939-43

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

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