Abstract:
:The cell-wall-less prokaryote Mycoplasma pneumoniae, long considered among the smallest and simplest cells capable of self-replication, has a distinct cellular polarity characterized by the presence of a differentiated terminal organelle which functions in adherence to human respiratory epithelium, gliding motility, and cell division. Characterization of hemadsorption (HA)-negative mutants has resulted in identification of several terminal organelle proteins, including P30, the loss of which results in developmental defects and decreased adherence to host cells, but their impact on M. pneumoniae gliding has not been investigated. Here we examined the contribution of P30 to gliding motility on the basis of satellite growth and cell gliding velocity and frequency. M. pneumoniae HA mutant II-3 lacking P30 was nonmotile, but HA mutant II-7 producing a truncated P30 was motile, albeit at a velocity 50-fold less than that of the wild type. HA-positive revertant II-3R producing an altered P30 was unexpectedly not fully wild type with respect to gliding. Complementation of mutant II-3 with recombinant wild-type and mutant alleles confirmed the correlation between gliding defect and loss or alteration in P30. Surprisingly, fusion of yellow fluorescent protein to the C terminus of P30 had little impact on cell gliding velocity and significantly enhanced HA. Finally, while quantitative examination of HA revealed clear distinctions among these mutant strains, gliding defects did not correlate strictly with the HA phenotype, and all strains attached to glass at wild-type levels. Taken together, these findings suggest a role for P30 in gliding motility that is distinct from its requirement in adherence.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hasselbring BM,Jordan JL,Krause DCdoi
10.1128/JB.187.18.6281-6289.2005keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
6281-9issue
18eissn
0021-9193issn
1098-5530pii
187/18/6281journal_volume
187pub_type
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journal_title:Journal of bacteriology
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abstract::Hertman, I. (Israel Institute for Biological Research, Ness Ziona, Israel). Bacteriophage common to Pasteurella pestis and Escherichia coli. J. Bacteriol. 88:1002-1005. 1964.-Phage Y of Pasteurella pestis and phage T(3) of Escherichia coli are serologically related and have common bacterial hosts. Anti-Y serum neutral...
journal_title:Journal of bacteriology
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doi:10.1128/JB.88.4.1002-1005.1964
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doi:10.1128/JB.120.1.74-80.1974
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5959-5970.1995
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abstract::Spectinomycin-resistant (Spcr) mutants of Escherichia coli were isolated from nutrient agar plates containing 20% sucrose and 100 mug of spectinomycin per ml. About one-third of the Spcr mutants thus obtained were sucrose dependent (Sucd) and were classified into two types: I, those unable to grow on sucrose-free medi...
journal_title:Journal of bacteriology
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doi:10.1128/JB.125.1.142-148.1976
更新日期:1976-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1158-1163.1973
更新日期:1973-06-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.176.22.7113-7114.1994
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1975-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5337-5346.1996
更新日期:1996-09-01 00:00:00
abstract:UNLABELLED:The latent period of phage T4, normally ∼25 min, can be extended indefinitely if the infected cell is superinfected after 5 min. This phenomenon, designated lysis inhibition (LIN), was first described in the 1940s and is genetically defined by mutations in diverse T4 r genes. RI, the main effector of LIN, ha...
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pub_type: 杂志文章
doi:10.1128/JB.104.1.27-33.1970
更新日期:1970-10-01 00:00:00
abstract::The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR. ...
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pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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更新日期:2006-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.671-679.1978
更新日期:1978-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.1038-1044.1983
更新日期:1983-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1976-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.841-858.1962
更新日期:1962-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1980-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2427-2430.1996
更新日期:1996-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.1.315-325.1972
更新日期:1972-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.6.1503-1513.2002
更新日期:2002-03-01 00:00:00