Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones.


:Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer defective). In this study, we examined the effects of overproduction of some molecular chaperones on the phenotypes of ftsH mutants. The growth retardation was partially suppressed by overproduction of GroEL/GroES (Hsp60/Hsp10) or HtpG (Hsp90), although these chaperones could not totally substitute for FtsH. Overproduction of HtpG specifically alleviated the Std phenotype, while that of GroEL/GroES alleviated the protein export defect of ftsH mutants. These results suggest that FtsH functions can be somehow compensated for when the cellular concentrations of some molecular chaperones increase.


J Bacteriol


Journal of bacteriology


Shirai Y,Akiyama Y,Ito K




Has Abstract


1996-02-01 00:00:00












  • Restriction fragment length polymorphism analysis of Rhizobium galegae strains.

    abstract::Total DNA of various Rhizobium galegae strains representing different geographical origins, and taxonomic divergence was digested with three restriction enzymes separately, Southern blotted, and hybridized with six heterologous probes. The sequence divergences for different pairwise comparisons were calculated from pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kaijalainen S,Lindström K

    更新日期:1989-10-01 00:00:00

  • Characterization of lipoprotein EnvA in Chlamydia psittaci 6BC.

    abstract::The primary sequence of the small cysteine-rich protein (EnvA) of Chlamydia psittaci 6BC has been shown to possess a potential lipid modification/signal peptidase II-processing site, and the mature protein was labeled by a [3H]palmitic acid precursor. We further characterized the mature EnvA, showing that it lacks the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Everett KD,Desiderio DM,Hatch TP

    更新日期:1994-10-01 00:00:00

  • Cloning and expression in Escherichia coli of the dnaK gene of Zymomonas mobilis.

    abstract::The DnaK protein of Zymomonas mobilis (DnaKz) was identified and found to be 80% identical to the DnaK protein of Escherichia coli on the basis of the sequence of the N-terminal 21 amino acids. The dnaKz gene was cloned and found to be expressed in a thermosensitive dnaK mutant of Escherichia coli. Expression of the f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Michel GP

    更新日期:1993-05-01 00:00:00

  • Carboxypeptidase S- and carboxypeptidase Y-deficient mutants of Saccharomyces cerevisiae.

    abstract::A new carboxypeptidase (carboxypeptidase S) was found in a Saccharomyces cerevisiae strain lacking carboxypeptidase Y (D. H. Wolf and U. Weiser, Eur. J. Biochem. 73:553-556, 1977). Mutants devoid of carboxypeptidase S activity were isolated from a mutant strain that was also deficient in carboxypeptidase Y. Four mutan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wolf DH,Ehmann C

    更新日期:1981-08-01 00:00:00

  • Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease.

    abstract::Mushin, Rose (University of Melbourne, Victoria, Australia) and Frances M. Ashburner. Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease. J. Bacteriol. 83:1260-1267. 1962.-The aerobic bacterial intestinal flora of mutton birds, healthy and affected with "limy" disease, wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-06-01 00:00:00

  • Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon.

    abstract::The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Slack FJ,Mueller JP,Sonenshein AL

    更新日期:1993-08-01 00:00:00

  • rec-2-dependent phage recombination in Haemophilus influenzae.

    abstract::The genetic transformation mutant Rd(DB117)rec- has a pleiotropic phenotype that includes reduced levels of phage recombination. Physical mapping experiments showed that this strain has a 78.5-kbp insertion in the rec-2 gene. The rec-2 dependence of phage recombination was reexamined to determine whether the defective...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kupfer DM,McCarthy D

    更新日期:1992-08-01 00:00:00

  • Transglycosylase and endopeptidase participate in the degradation of murein during autolysis of Escherichia coli.

    abstract::The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kitano K,Tuomanen E,Tomasz A

    更新日期:1986-09-01 00:00:00

  • Effect of site-specific mutations in different phosphotransfer domains of the chemosensory protein ChpA on Pseudomonas aeruginosa motility.

    abstract::The virulence of Pseudomonas aeruginosa and other surface pathogens involves the coordinate expression of a wide range of virulence determinants, including type IV pili. These surface filaments are important for the colonization of host epithelial tissues and mediate bacterial attachment to, and translocation across, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leech AJ,Mattick JS

    更新日期:2006-12-01 00:00:00

  • Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer.

    abstract::Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Crasta KC,Chua KL,Subramaniam S,Frey J,Loh H,Tan HM

    更新日期:2002-04-01 00:00:00


    abstract::Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: ALTENBERN RA

    更新日期:1963-03-01 00:00:00

  • Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1.

    abstract::Pseudomonas aeruginosa and many other bacteria can utilize biogenic polyamines, including diaminopropane (DAP), putrescine (Put), cadaverine (Cad), and spermidine (Spd), as carbon and/or nitrogen sources. Transcriptome analysis in response to exogenous Put and Spd led to the identification of a list of genes encoding ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yao X,He W,Lu CD

    更新日期:2011-08-01 00:00:00

  • Growth kinetics of extremely halophilic archaea (family halobacteriaceae) as revealed by arrhenius plots.

    abstract::Members of the family Halobacteriaceae in the domain Archaea are obligate extreme halophiles. They occupy a variety of hypersaline environments, and their cellular biochemistry functions in a nearly saturated salty milieu. Despite extensive study, a detailed analysis of their growth kinetics is missing. To remedy this...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Robinson JL,Pyzyna B,Atrasz RG,Henderson CA,Morrill KL,Burd AM,Desoucy E,Fogleman RE 3rd,Naylor JB,Steele SM,Elliott DR,Leyva KJ,Shand RF

    更新日期:2005-02-01 00:00:00

  • Immunocytochemical localization of a calmodulinlike protein in Bacillus subtilis cells.

    abstract::To determine possible functions of the calmodulinlike protein of Bacillus subtilis, the time course of its expression during sporulation and its cellular localization were studied. The protein was expressed in a constitutive manner from the end of logarithmic growth through 8 h of sporulation as determined by antibody...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dominguez DC,Adams H,Hageman JH

    更新日期:1999-08-01 00:00:00

  • Growth-phase-dependent immunodeterminants of Rhizobium trifolii lipopolysaccharide which bind trifoliin A, a white clover lectin.

    abstract::The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to purified LPS and cells in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hrabak EM,Urbano MR,Dazzo FB

    更新日期:1981-11-01 00:00:00

  • Chromosome condensation in the absence of the non-SMC subunits of MukBEF.

    abstract::MukBEF is a bacterial SMC (structural maintenance of chromosome) complex required for chromosome partitioning in Escherichia coli. We report that overproduction of MukBEF results in marked chromosome condensation. This condensation is rapid and precedes the effects of overproduction on macromolecular synthesis. Conden...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang Q,Mordukhova EA,Edwards AL,Rybenkov VV

    更新日期:2006-06-01 00:00:00

  • Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate.

    abstract::Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Grunberg E,Cleeland R

    更新日期:1966-07-01 00:00:00

  • Deficiency of the Rgg regulator promotes H2O2 resistance, AhpCF-mediated H2O2 decomposition, and virulence in Streptococcus pyogenes.

    abstract::Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pulliainen AT,Hytönen J,Haataja S,Finne J

    更新日期:2008-05-01 00:00:00

  • Low-molecular-weight protein tyrosine phosphatases of Bacillus subtilis.

    abstract::In gram-negative organisms, enzymes belonging to the low-molecular-weight protein tyrosine phosphatase (LMPTP) family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. LMPTPs have been identified also in gram-positive bacteria,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Musumeci L,Bongiorni C,Tautz L,Edwards RA,Osterman A,Perego M,Mustelin T,Bottini N

    更新日期:2005-07-01 00:00:00

  • Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of sn-glycerol 3-phosphate acyltransferase Km mutants.

    abstract::plsB mutants of Escherichia coli are sn-glycerol 3-phosphate auxotrophs which owe their requirement to a K(m) defect in sn-glycerol 3-phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. We have located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cronan JE Jr,Bell RM

    更新日期:1974-10-01 00:00:00

  • The genome-sequenced variant of Campylobacter jejuni NCTC 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes.

    abstract::The genome sequence of the enteric bacterial pathogen Campylobacter jejuni NCTC 11168 (11168-GS) was published in 2000, providing a valuable resource for the identification of C. jejuni-specific colonization and virulence factors. Surprisingly, the 11168-GS clone was subsequently found to colonize 1-day-old chicks fol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gaynor EC,Cawthraw S,Manning G,MacKichan JK,Falkow S,Newell DG

    更新日期:2004-01-01 00:00:00

  • Molecular characterization of the Lactococcus lactis LlaKR2I restriction-modification system and effect of an IS982 element positioned between the restriction and modification genes.

    abstract::The nucleotide sequence of the plasmid-encoded LlaKR2I restriction-modification (R-M) system of Lactococcus lactis subsp. lactis biovar diacetylactis KR2 was determined. This R-M system comprises divergently transcribed endonuclease (llaKR2IR) and methyltransferase (llaKR2IM) genes; located in the intergenic region is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Twomey DP,McKay LL,O'Sullivan DJ

    更新日期:1998-11-01 00:00:00

  • Redox signal transduction by the ArcB sensor kinase of Haemophilus influenzae lacking the PAS domain.

    abstract::The Arc (anoxic redox control) two-component signal transduction system of Escherichia coli, which comprises the tripartite ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous operons in response to redox conditions of growth. We demonstrate that the arcA and arcB genes of Haemophi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Georgellis D,Kwon O,Lin EC,Wong SM,Akerley BJ

    更新日期:2001-12-01 00:00:00

  • Regulation of metalloprotease gene expression in Vibrio vulnificus by a Vibrio harveyi LuxR homologue.

    abstract::Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyi LuxR-like transcriptional act...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shao CP,Hor LI

    更新日期:2001-02-01 00:00:00

  • Resuscitation of a defective prophage in Salmonella cocultures.

    abstract::Widely studied Salmonella enterica serovar Typhimurium strains ATCC 14028s and SL1344 harbor a cryptic ST64B prophage unable to produce infectious virions. We found that coculturing either strain with an isogenic sibling lacking the prophage leads to the appearance of active forms of the virus. Active phage originates...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Figueroa-Bossi N,Bossi L

    更新日期:2004-06-01 00:00:00

  • Immunochemistry of the cell walls of Listeria monocytogenes.

    abstract::The antigenic specificity of Listeria monocytogenes types I, II, III, IVa, and IVb was studied by immunochemical techniques. Immunologically active carbohydrates of the various types were extracted from cell walls and were chemically analyzed. Types I and II contained predominantly glucosamine and rhamnose; type III, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ullmann WW,Cameron JA

    更新日期:1969-05-01 00:00:00

  • Regulation of fructose uptake and catabolism by succinate in Azospirillum brasilense.

    abstract::Fructose uptake and catabolism in Azospirillum brasilense is dependent on three fructose-inducible enzymes (fru-enzymes): (i) enzyme I and (ii) enzyme II of the phosphoenolpyruvate:fructose phosphotransferase system and (iii) 1-phosphofructokinase. In minimal medium containing 3.7 mM succinate and 22 mM fructose as so...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mukherjee A,Ghosh S

    更新日期:1987-09-01 00:00:00

  • The catabolite control protein CcpA binds to Pmga and influences expression of the virulence regulator Mga in the Group A streptococcus.

    abstract::Carbon catabolite repression (CCR) allows bacteria to alter metabolism in response to the availability of specific sugar sources, and increasing evidence suggests that CCR is involved in regulating virulence gene expression in many pathogens. A scan of the M1 SF370 group A streptococcus (GAS) genome using a Bacillus s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Almengor AC,Kinkel TL,Day SJ,McIver KS

    更新日期:2007-12-01 00:00:00

  • Addition of Escherichia coli K-12 growth observation and gene essentiality data to the EcoCyc database.

    abstract::The sets of compounds that can support growth of an organism are defined by the presence of transporters and metabolic pathways that convert nutrient sources into cellular components and energy for growth. A collection of known nutrient sources can therefore serve both as an impetus for investigating new metabolic pat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mackie A,Paley S,Keseler IM,Shearer A,Paulsen IT,Karp PD

    更新日期:2014-03-01 00:00:00

  • Valinomycin-induced cation transport in vesicles does not reflect the activity of K+ transport systems in Escherichia coli.

    abstract::Transport systems for K+ in Escherichia coli are not detectable in membrane vesicles, but vesicles will take up K+ (and Rb+) in the presence of valinomycin. It is generally believed that valinomycin acts as a lipid-soluble cation carrier and that it does not interact with or activate cation transport systems. This vie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Altendorf K,Epstein W,Löhmann A

    更新日期:1986-04-01 00:00:00