Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones.


:Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer defective). In this study, we examined the effects of overproduction of some molecular chaperones on the phenotypes of ftsH mutants. The growth retardation was partially suppressed by overproduction of GroEL/GroES (Hsp60/Hsp10) or HtpG (Hsp90), although these chaperones could not totally substitute for FtsH. Overproduction of HtpG specifically alleviated the Std phenotype, while that of GroEL/GroES alleviated the protein export defect of ftsH mutants. These results suggest that FtsH functions can be somehow compensated for when the cellular concentrations of some molecular chaperones increase.


J Bacteriol


Journal of bacteriology


Shirai Y,Akiyama Y,Ito K




Has Abstract


1996-02-01 00:00:00












  • Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors.

    abstract::Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gregory MA,Till R,Smith MC

    更新日期:2003-09-01 00:00:00

  • Characteristics of cold-sensitive mutants of Escherichia coli K-12 defective in deoxyribonucleic acid replication.

    abstract::Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wehr CT,Waskell L,Glaser DA

    更新日期:1975-01-01 00:00:00

  • Surface structure of streptomycete spores as revealed by negative staining and freeze-etching.

    abstract::The application of a simple negative staining technique and freeze-etching has revealed detailed information on the species-specific surface structure of spores and aerial hyphae of streptomycetes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wildermuth H

    更新日期:1970-01-01 00:00:00

  • Replication of the bacteriocinogenic plasmid Clo DF13 in thermosensitive Escherichia coli mutants defective in initiation or elongation of deoxyribonucleic acid replication.

    abstract::The replication of the bacteriocinogenic plasmid Clo DF13 has been studied in the seven temperature-sensitive Escherichia coli mutants defective in deoxyribonucleic acid (DNA) replication (dnaA-dnaG). Experiments with dna initiation mutants revealed that the replication of the Clo DF13 plasmid depends to a great exten...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Veltkamp E,Nijkamp HJ

    更新日期:1974-12-01 00:00:00

  • Characterization of the genes and proteins of a two-component system from the hyperthermophilic bacterium Thermotoga maritima.

    abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee PJ,Stock AM

    更新日期:1996-10-01 00:00:00

  • Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.

    abstract::Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Metcalf WW,Steed PM,Wanner BL

    更新日期:1990-06-01 00:00:00

  • Complete nucleotide sequence of Tn10.

    abstract::The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chalmers R,Sewitz S,Lipkow K,Crellin P

    更新日期:2000-05-01 00:00:00

  • Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase.

    abstract::The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pradel E,Marck C,Boquet PL

    更新日期:1990-02-01 00:00:00

  • High-molecular-weight complexes of RsbR and paralogues in the environmental signaling pathway of Bacillus subtilis.

    abstract::Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsibl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Delumeau O,Chen CC,Murray JW,Yudkin MD,Lewis RJ

    更新日期:2006-11-01 00:00:00

  • Escherichia coli pfs transcription: regulation and proposed roles in autoinducer-2 synthesis and purine excretion.

    abstract::Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcripti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kim Y,Lew CM,Gralla JD

    更新日期:2006-11-01 00:00:00

  • Structural and genetic analysis of X-ray scattering by spores of Bacillus subtilis.

    abstract::Dormant spores of Bacillus subtilis exhibit two prominent X-ray scattering peaks. These peaks persisted in spores lacking most alpha/beta-type small, acid-soluble protein or the CotE protein responsible for assembly of much spore coat protein, but they were absent from spores of strains lacking the late sporulation-sp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Qiu X,Setlow P

    更新日期:2009-12-01 00:00:00

  • Evidence for two phosphonate degradative pathways in Enterobacter aerogenes.

    abstract::We screened mini-Mu plasmid libraries from Enterobacter aerogenes IFO 12010 for plasmids that complement Escherichia coli phn mutants that cannot use phosphonates (Pn) as the sole source of phosphorus (P). We isolated two kinds of plasmids that, unexpectedly, encode genes for different metabolic pathways. One kind com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee KS,Metcalf WW,Wanner BL

    更新日期:1992-04-01 00:00:00

  • A DNA adenine methyltransferase of Escherichia coli that is cell cycle regulated and essential for viability.

    abstract::DNA sequence analysis revealed that the putative yhdJ DNA methyltransferase gene of Escherichia coli is 55% identical to the Nostoc sp. strain PCC7120 gene encoding DNA methyltransferase AvaIII, which methylates adenine in the recognition sequence, ATGCAT. The yhdJ gene was cloned, and the enzyme was overexpressed and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,收录出版


    authors: Kossykh VG,Lloyd RS

    更新日期:2004-04-01 00:00:00

  • Influence of temperature on tRNA modification in archaea: Methanococcoides burtonii (optimum growth temperature [Topt], 23 degrees C) and Stetteria hydrogenophila (Topt, 95 degrees C).

    abstract::We report the first study of tRNA modification in psychrotolerant archaea, specifically in the archaeon Methanococcoides burtonii grown at 4 and 23 degrees C. For comparison, unfractionated tRNA from the archaeal hyperthermophile Stetteria hydrogenophila cultured at 93 degrees C was examined. Analysis of modified nucl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Noon KR,Guymon R,Crain PF,McCloskey JA,Thomm M,Lim J,Cavicchioli R

    更新日期:2003-09-01 00:00:00

  • Bacteriophage T4 self-assembly: in vitro reconstitution of recombinant gp2 into infectious phage.

    abstract::T4 gene 2 mutants have a pleiotropic phenotype: degradation of injected phage DNA by exonuclease V (ExoV) in the recBCD(+) host cell cytoplasm and a low burst size due, at least in part, to a decreased ability for head-to-tail (H-T) joining. The more N terminal the mutation, the more pronounced is the H-T joining defe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang GR,Vianelli A,Goldberg EB

    更新日期:2000-02-01 00:00:00

  • Functional organization of the autotransporter adhesin involved in diffuse adherence.

    abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Charbonneau ME,Mourez M

    更新日期:2007-12-01 00:00:00

  • Production of nitrous oxide from nitrite in Klebsiella pneumoniae: mutants altered in nitrogen metabolism.

    abstract::Under anaerobic conditions, Klebsiella pneumoniae reduced nitrite (NO2-), yielding nitrous oxide (N2O) and ammonium ions (NH4+) as products. Nitrous oxide formation accounted for about 5% of the total NO2- reduced, and NH4+ production accounted for the remainder. Glucose and pyruvate were the electron donors for NO2- ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Satoh T,Hom SS,Shanmugam KT

    更新日期:1983-08-01 00:00:00


    abstract::Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: ROMANO AH,GEASON DJ

    更新日期:1964-10-01 00:00:00

  • Opposite and Coordinated Rotation of Amphitrichous Flagella Governs Oriented Swimming and Reversals in a Magnetotactic Spirillum.

    abstract:UNLABELLED:Current knowledge regarding the mechanism that governs flagellar motor rotation in response to environmental stimuli stems mainly from the study of monotrichous and peritrichous bacteria. Little is known about how two polar flagella, one at each cell pole of the so-called amphitrichous bacterium, are coordin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Murat D,Hérisse M,Espinosa L,Bossa A,Alberto F,Wu LF

    更新日期:2015-10-01 00:00:00

  • A simplified methylcoenzyme M methylreductase assay with artificial electron donors and different preparations of component C from Methanobacterium thermoautotrophicum delta H.

    abstract::Different preparations of the methylreductase were tested in a simplified methylcoenzyme M methylreductase assay with artificial electron donors under a nitrogen atmosphere. ATP and Mg2+ stimulated the reaction. Tris(2,2'-bipyridine)ruthenium (II), chromous chloride, chromous acetate, titanium III citrate, 2,8-diamino...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hartzell PL,Escalante-Semerena JC,Bobik TA,Wolfe RS

    更新日期:1988-06-01 00:00:00

  • Cyclic diguanylic acid and cellulose synthesis in Agrobacterium tumefaciens.

    abstract::The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Amikam D,Benziman M

    更新日期:1989-12-01 00:00:00

  • Genetic and segregation analysis of Escherichia coli strains containing a tandem duplication of the trpD-purB region of the chromosome.

    abstract::Genetic and segregation analysis of Escherichia coli strains containing a partial duplication of the trp operon reveal that the 2.5-min-long region trpD-purB is duplicated in tandem in the chromosome. The adjacent loci cysB and fabD are not duplicated. Although one copy of the duplicated region is longer than the maxi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Simonian MH,Goldstein RV,Mosteller RD

    更新日期:1978-02-01 00:00:00

  • Sortase B, a new class of sortase in Listeria monocytogenes.

    abstract::Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bierne H,Garandeau C,Pucciarelli MG,Sabet C,Newton S,Garcia-del Portillo F,Cossart P,Charbit A

    更新日期:2004-04-01 00:00:00

  • Two amino acid residues of transposase contributing to differential transposability of IS1 elements in Escherichia coli.

    abstract::Escherichia coli W3110 contains four types of IS1 elements in the chromosome. Using an insertion element entrapping system, we collected 116 IS1 plasmid insertion mutants, which resulted from a minimum of 26 independent IS1 insertion events. All of them had insertions of IS1 of the IS1A (IS1E and IS1G) type. Inspectio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Chen JH,Hsu WB,Hwang JL

    更新日期:1998-10-01 00:00:00


    abstract::Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295-1299. 1963.-A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1963-06-01 00:00:00

  • Transcriptional phase variation of a type III restriction-modification system in Helicobacter pylori.

    abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: de Vries N,Duinsbergen D,Kuipers EJ,Pot RG,Wiesenekker P,Penn CW,van Vliet AH,Vandenbroucke-Grauls CM,Kusters JG

    更新日期:2002-12-01 00:00:00

  • Identification of a segment of the Escherichia coli Tsx protein that functions as a bacteriophage receptor area.

    abstract::The Escherichia coli outer membrane protein Tsx functions as a nucleoside-specific channel and serves as the receptor for colicin K and a number of T-even-type bacteriophages, including phage T6. To identify those segments of the Tsx protein that are important for its phage receptor function, we devised a selection an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schneider H,Fsihi H,Kottwitz B,Mygind B,Bremer E

    更新日期:1993-05-01 00:00:00

  • Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea.

    abstract::Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An endoribonucleolytic activity from the extreme halophile Haloarcula marismortui showed the same RNA substrate specificity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Franzetti B,Sohlberg B,Zaccai G,von Gabain A

    更新日期:1997-02-01 00:00:00

  • Arm-in-Arm Response Regulator Dimers Promote Intermolecular Signal Transduction.

    abstract:UNLABELLED:Bacteriophytochrome photoreceptors (BphPs) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. Most of these systems utilize single-domain response regulators to transduce signal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Baker AW,Satyshur KA,Moreno Morales N,Forest KT

    更新日期:2016-03-31 00:00:00

  • Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli.

    abstract::A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee JH,Wendt JC,Shanmugam KT

    更新日期:1990-04-01 00:00:00