Abstract:
:Frampton, E. W. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston), and B. R. Brinkley. Evidence of lysogeny in derivatives of Escherichia coli. J. Bacteriol. 90:446-452. 1965.-Exposure of cultures of several thymine-requiring derivatives of Escherichia coli strain 15 to radiation [X rays or ultraviolet (UV)] resulted in an increased synthesis of ribonucleic acid (RNA) as measured by the incorporation of radioactive uridine. In addition to radiation, mitomycin C (5 or 25 mug/ml) or growth in the absence of thymine also stimulated RNA synthesis in cultures in which the bulk of RNA synthesis was terminated by a shift-down in growth conditions. RNA purified from X-irradiated bacteria was resolved on columns of methylated serum albumin and yielded an elution profile which appeared similar to RNA synthesized by phage-infected bacteria. Electron microscopic examination of cultures induced to lyse by exposure to UV radiation, or by growth in the presence of 5-bromouracil before exposure to radiation (X rays or UV), showed the presence of mostly incomplete phage particles.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
FRAMPTON EW,BRINKLEY BRdoi
10.1128/JB.90.2.446-452.1965keywords:
subject
Has Abstractpub_date
1965-08-01 00:00:00pages
446-52eissn
0021-9193issn
1098-5530journal_volume
90pub_type
杂志文章abstract::Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1314-1322.1978
更新日期:1978-03-01 00:00:00
abstract::The process of flagellar assembly in Salmonella typhimurium was investigated by using temperature-sensitive mutants. The mutants were grown at the restrictive temperature and then at the permissive temperature, with radiolabel supplied in the first phase of the experiment and not the second, or vice versa. Flagellar h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.3.1327-1339.1990
更新日期:1990-03-01 00:00:00
abstract::Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3450-3457.1987
更新日期:1987-08-01 00:00:00
abstract::The conjugative plasmid pTR2030 has been used extensively to confer phage resistance in commercial Lactococcus starter cultures. The plasmid harbors a 16-kb region, flanked by insertion sequence (IS) elements, that encodes the restriction/modification system LlaI and carries an abortive infection gene, abiA. The AbiA ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00904-06
更新日期:2007-02-01 00:00:00
abstract::In untreated cells of the marine pseudomonad studied here, alkaline phosphatase was found to be located in the periplasmic space, at the cell surface, and in the medium into which it had been shed during growth. Washing in 0.5 M NaCl, which removed the loosely bound outer layer, caused a shift of periplasmic enzyme to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1281-1293.1973
更新日期:1973-06-01 00:00:00
abstract::ComX and CSF are Bacillus subtilis extracellular signaling peptides. Many different strains of B. subtilis do not communicate due to strain-specific variation of ComX. We demonstrate that CSF is a species-specific signaling molecule that partially compensates for the lack of ComX-mediated communication between differe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00187-08
更新日期:2008-06-01 00:00:00
abstract::Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02136-12
更新日期:2013-03-01 00:00:00
abstract::Penicillium chrysogenum is an important producer of penicillin antibiotics. A key step in their biosynthesis is the oxidative cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N by the enzyme isopenicillin N synthase (IPNS). bis-ACV, the oxidized disulfide form of ACV is, however, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.973-984.1994
更新日期:1994-02-01 00:00:00
abstract::We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2612-2619.1992
更新日期:1992-04-01 00:00:00
abstract::The rate at which the peptidoglycan precursor meso-diaminopimelic acid (DAP) is incorporated into the cell wall of Escherichia coli cells was determined by pulse-label experiments. For different E. coli strains, the incorporation rate was compared with the rate of uptake of DAP into the cell. With E. coli W7, a dap ly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.331-337.1985
更新日期:1985-10-01 00:00:00
abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05454-11
更新日期:2011-10-01 00:00:00
abstract::In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2975-2977.1996
更新日期:1996-05-01 00:00:00
abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4417-4425.1987
更新日期:1987-10-01 00:00:00
abstract::Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.3.629-638.1968
更新日期:1968-09-01 00:00:00
abstract::A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1329-1338.1983
更新日期:1983-06-01 00:00:00
abstract::Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2177-2186.1987
更新日期:1987-05-01 00:00:00
abstract::Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.13.4052-4060.2001
更新日期:2001-07-01 00:00:00
abstract::Although nitrate stimulated the capacity of Clostridium thermoautotrophicum and Clostridium thermoaceticum to oxidize (utilize) substrates under heterotrophic conditions, it inhibited autotrophic H2-CO2-dependent growth. Under basal medium conditions, nitrate was also inhibitory to the use of one-carbon substrates (i....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4597-4603.1996
更新日期:1996-08-01 00:00:00
abstract::The yeast Saccharomyces cerevisiae has two separate genes (APT1 and APT2) that encode two potentially different forms of adenine phosphoribosyltransferase (APRT). However, genetic analysis indicated that only APT1 could code for a complementing activity. Cloning and expression of both the APT1 and APT2 genes in Escher...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.1.347-352.1999
更新日期:1999-01-01 00:00:00
abstract::Two different 30-nucleotide regions of the cryIAc insecticidal protoxin gene from Bacillus thuringiensis were randomly mutagenized. One region was within one of seven amphipathic helices believed to be important for the formation of ion channels. There was no loss of toxicity for three test insects by any of 27 mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.4059-4065.1995
更新日期:1995-07-01 00:00:00
abstract::Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. Defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiolo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05695-11
更新日期:2011-11-01 00:00:00
abstract::Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3256-3261.1999
更新日期:1999-05-01 00:00:00
abstract::Fixation of NaH(14)CO(3) by a heavy cell suspension of Streptococcus faecalis var. liquefaciens was studied. Several nutrients, pyridoxal, riboflavine, adenine, uracil, and O(2) stimulated (14)CO(2) incorporation into cells only under conditions that were adequate for synthesis of cell macromolecules. Biotin increased...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.27-33.1970
更新日期:1970-10-01 00:00:00
abstract::2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase (BphD) is a key determinant in the aerobic transformation of polychlorinated biphenyls (PCBs) by Burkholderia sp. strain LB400 (S. Y. K. Seah, G. Labbé, S. Nerdinger, M. Johnson, V. Snieckus, and L. D. Eltis, J. Biol. Chem. 275:15701-15708, 2000). To determin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.5.1511-1516.2001
更新日期:2001-03-01 00:00:00
abstract::Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.623-630.1977
更新日期:1977-08-01 00:00:00
abstract::Mycoplasmas that are known to exhibit gliding motility possess a differentiated tip structure. This polar organelle mediates cytadherence and gliding motor activity and contains a cytoskeleton-like component that provides structural support. Here, we describe gliding motility and a unique cytoskeleton in Mycoplasma in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00474-09
更新日期:2009-08-01 00:00:00
abstract::The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01289-08
更新日期:2009-02-01 00:00:00
abstract::We have recently established that the facultative phototrophic bacterium Rhodobacter sphaeroides, like the closely related Rhodobacter capsulatus species, contains both the previously characterized mobile electron carrier cytochrome c2 (cyt c2) and the more recently discovered membrane-anchored cyt cy. However, R. sph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.2013-2024.2001
更新日期:2001-03-01 00:00:00
abstract::Spore formation of Myxococcus xanthus can occur not only on agar plates during fruiting body formation, but also in a liquid culture by simply adding glycerol, dimethyl sulfoxide, or phenethyl alcohol to the culture. This chemically-induced spore formation occurs synchronously and much faster than that occurring durin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.1076-1082.1980
更新日期:1980-12-01 00:00:00
abstract::A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.212-218.1975
更新日期:1975-01-01 00:00:00