Comparative specificities of two evolutionarily divergent hydrolases involved in microbial degradation of polychlorinated biphenyls.

abstract：:Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...

journal_title：Journal of bacteriology

authors： Pedrotta V,Witholt B

更新日期：1999-05-01 00:00:00

abstract：:Fast-growing species of Rhizobium were found to secrete low-molecular-weight beta-2-linked glucans when cultured in synthetic liquid medium. These glucans are quite similar to beta-2-linked glucans produced by species of Agrobacterium. No reducing terminus was detected in these glucans. ...

journal_title：Journal of bacteriology

authors： York WS,McNeil M,Darvill AG,Albersheim P

更新日期：1980-04-01 00:00:00

abstract：:The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

更新日期：2004-12-01 00:00:00

abstract：:Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

journal_title：Journal of bacteriology

authors： Calhoun DH,Feary TW

更新日期：1969-01-01 00:00:00

abstract：:We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural ...

journal_title：Journal of bacteriology

authors： Hunke S,Landmesser H,Schneider E

更新日期：2000-03-01 00:00:00

abstract：:We cloned the cryptic phn operon of a K-12 strain, phn(EcoK), and analyzed the nucleotide sequence of the phn region (11,672 bp). An mRNA start site upstream of the phnC gene was identified by S1 nuclease mapping. The pho regulon activator PhoB protects a pho box region near the mRNA start in DNase I footprinting and ...

journal_title：Journal of bacteriology

authors： Makino K,Kim SK,Shinagawa H,Amemura M,Nakata A

更新日期：1991-04-01 00:00:00

abstract：:Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...

journal_title：Journal of bacteriology

authors： Kim KS,Barksdale L

更新日期：1969-06-01 00:00:00

abstract：:Protein synthesis in Allomyces marcogynus mitospores began at the time of encystment and was required for further germination. The rate of protein synthesis in germinating spores decreased in the absence of ribonucleic acid synthesis relative to the uninhibited control. There was a concomitant decrease in polysome con...

journal_title：Journal of bacteriology

authors： Smith BA,Burke DD

更新日期：1980-09-01 00:00:00

abstract：:Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...

journal_title：Journal of bacteriology

authors： Pucci MJ,Daneo-Moore L

更新日期：1984-08-01 00:00:00

abstract：:Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...

journal_title：Journal of bacteriology

authors： Kilstrup M,Meng LM,Neuhard J,Nygaard P

更新日期：1989-04-01 00:00:00

abstract：:Tests for utilization of d-octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium. Among 46 virulent strains of Agrobacterium, only two strains were found which utiliz...

journal_title：Journal of bacteriology

authors： Lippincott JA,Beiderbeck R,Lippincott BB

更新日期：1973-10-01 00:00:00

abstract：:AraC protein, a transcriptional regulator of the L-arabinose operon in Escherichia coli, is dimeric. Each monomer consists of a domain for DNA binding plus transcription activation and a domain for dimerization plus arabinose binding. These are connected to one another by a linker region of at least 5 amino acids. Her...

journal_title：Journal of bacteriology

authors： Eustance RJ,Schleif RF

更新日期：1996-12-01 00:00:00

abstract：:In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into th...

journal_title：Journal of bacteriology

authors： Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

更新日期：1993-10-01 00:00:00

abstract：:The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...

journal_title：Journal of bacteriology

authors： Sarvas MO,Palva IA

更新日期：1983-08-01 00:00:00

abstract：:The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...

journal_title：Journal of bacteriology

authors： Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

更新日期：1988-10-01 00:00:00

abstract：:Maintaining cell envelope integrity is critical for bacterial survival, including bacteria living in a complex and dynamic environment such as the human oral cavity. Streptococcus mutans, a major etiological agent of dental caries, uses two-component signal transduction systems (TCSTSs) to monitor and respond to vario...

journal_title：Journal of bacteriology

authors： Suntharalingam P,Senadheera MD,Mair RW,Lévesque CM,Cvitkovitch DG

更新日期：2009-05-01 00:00:00

abstract：:The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW ...

journal_title：Journal of bacteriology

authors： Sanders DA,Mendez B,Koshland DE Jr

更新日期：1989-11-01 00:00:00

abstract：:Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...

journal_title：Journal of bacteriology

authors： Oertel W,Goulian M

更新日期：1979-11-01 00:00:00

abstract：:Mu promoters active during the lytic cycle were located by isolating RNA at various times after induction of Mu prophages, radiolabeling it by capping in vitro, and hybridizing it to Mu DNA fragments on Southern blots. Signals were detected from four new promoters in addition to the previously characterized Pe (early)...

journal_title：Journal of bacteriology

authors： Stoddard SF,Howe MM

更新日期：1989-06-01 00:00:00

abstract：:Soluble c-type cytochromes are central to metabolism of C1 compounds in methylotrophic bacteria. In order to characterize the role of c-type cytochromes in methane-utilizing bacteria (methanotrophs), we have purified four different cytochromes, cytochromes c-554, c-553, c-552, and c-551, from the marine methanotroph M...

journal_title：Journal of bacteriology

authors： DiSpirito AA,Lipscomb JD,Lidstrom ME

更新日期：1990-09-01 00:00:00

abstract：:Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunological techniques, we have compared the synthesis of the phoA protein (alkaline phosphatase) and the phoS protein (phosphate-binding protein) in response to the level of phosphate in the medium in different genetic backgrounds containing the kn...

journal_title：Journal of bacteriology

authors： Willsky GR,Malamy MH

更新日期：1976-07-01 00:00:00

abstract：:A late-log-phase culture of an Escherichia coli nadB pncA double mutant took up 6-[7-14C]aminonicotinic acid and excreted 6-[14C]aminonicotinamide. This mutant also accumulated intracellularly several radioactive compounds which have been tentatively identified as 6-amino analogs of compounds in the pyridine nucleotid...

journal_title：Journal of bacteriology

authors： Cobb JR,Pearcy SC,Gholson RK

更新日期：1977-09-01 00:00:00

abstract：:Monoubiquitination of the 12-transmembrane segment (12-TMS) Saccharomyces cerevisiae maltose transporter promoted the maximal internalization rate of this protein. This modification is similar to that of the 7-TMS alpha-factor receptor but different from that of the 12-TMS uracil and general amino acid permeases. This...

journal_title：Journal of bacteriology

authors： Lucero P,Peñalver E,Vela L,Lagunas R

更新日期：2000-01-01 00:00:00

abstract：:The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...

journal_title：Journal of bacteriology

authors： Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

更新日期：1999-04-01 00:00:00

abstract：:Three Escherichia coli phages, TuIa, TuIb, and TuII, were isolated from local sewage. We present evidence that they use the major outer membrane proteins Ia, Ib, and II, respectively, as receptors. In all cases the proteins, under the experimental conditions used, required lipopolysaccharide to exhibit their receptor ...

journal_title：Journal of bacteriology

authors： Datta DB,Arden B,Henning U

更新日期：1977-09-01 00:00:00

abstract：:A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...

journal_title：Journal of bacteriology

authors： Bankaitis VA,Kline EL

更新日期：1981-08-01 00:00:00

abstract：:Lactococcus lactis, a gram-positive organism widely used in the food industry, is a potential candidate for the secretion of biologically useful proteins. We examined the secretion efficiency and capacity of L. lactis by using the Staphylococcus aureus nuclease (Nuc) as a heterologous model protein. When expressed in ...

journal_title：Journal of bacteriology

authors： Le Loir Y,Gruss A,Ehrlich SD,Langella P

更新日期：1998-04-01 00:00:00

abstract：:The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

journal_title：Journal of bacteriology

authors： Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

更新日期：2014-01-01 00:00:00

abstract：:The virulence of Pseudomonas aeruginosa and other surface pathogens involves the coordinate expression of a wide range of virulence determinants, including type IV pili. These surface filaments are important for the colonization of host epithelial tissues and mediate bacterial attachment to, and translocation across, ...

journal_title：Journal of bacteriology

authors： Leech AJ,Mattick JS

更新日期：2006-12-01 00:00:00

abstract：:In vitro, archaeal SRP54 binds SRP RNA in the absence of SRP19, suggesting the latter to be expendable in Archaea. Accordingly, the Haloferax volcanii SRP19 gene was deleted. Although normally transcribed at a level comparable to that of the essential SRP54 gene, SRP19 deletion had no effect on cell growth, membrane p...

journal_title：Journal of bacteriology

authors： Yurist S,Dahan I,Eichler J

更新日期：2007-01-01 00:00:00