An amino acid substitution in penicillin-binding protein 3 creates pointed polar caps in Escherichia coli.

Abstract:

:The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which has never been observed before; the polar caps are pointed. We show that the substitution of amino acid Asn-361 by Ser, previously shown to be responsible for increased cephalexin resistance and for temperature sensitivity, causes the pointed polar caps. However, comparison of the morphological and physiological characteristics of the pbpBr1 mutant with those of other pbpB mutants suggests that the formation of pointed polar caps is not correlated with temperature sensitivity or cephalexin resistance. Partial inactivation of PBP3 by subinhibitory concentrations of cephalexin, furazlocillin, and piperacillin resulted in the formation of slightly pointed polar caps, suggesting that the shape of the polar caps is correlated with PBP3 activity. The large change in the shape of the polar caps was accompanied by a small change in the kinetics of peptidoglycan synthesis and in the local rate of surface synthesis activity along the cell envelope.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

doi

10.1128/jb.170.10.4828-4837.1988

subject

Has Abstract

pub_date

1988-10-01 00:00:00

pages

4828-37

issue

10

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

杂志文章
  • Analysis of the regulation of gene expression during Bacillus subtilis sporulation by manipulation of the copy number of spo-lacZ fusions.

    abstract::The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.3.1260-1266.1987

    authors: Piggot PJ,Curtis CA

    更新日期:1987-03-01 00:00:00

  • IS257-mediated cointegration in the evolution of a family of staphylococcal trimethoprim resistance plasmids.

    abstract::Analyses of the Staphylococcus epidermidis multiresistance plasmids pSK697 and pSK818 have revealed them to be closely related to the trimethoprim resistance plasmid pSK639, also isolated from S. epidermidis. pSK697 and pSK818 were found to contain a cointegrated copy of a second plasmid related to the S. epidermidis ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.20.6070-6073.1996

    authors: Leelaporn A,Firth N,Paulsen IT,Skurray RA

    更新日期:1996-10-01 00:00:00

  • Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein.

    abstract::Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1499-1503.2001

    authors: Davis MS,Cronan JE Jr

    更新日期:2001-02-01 00:00:00

  • A homologue of the 3-oxoacyl-(acyl carrier protein) synthase III gene located in the glycosylation island of Pseudomonas syringae pv. tabaci regulates virulence factors via N-acyl homoserine lactone and fatty acid synthesis.

    abstract::Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modificat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00763-06

    authors: Taguchi F,Ogawa Y,Takeuchi K,Suzuki T,Toyoda K,Shiraishi T,Ichinose Y

    更新日期:2006-12-01 00:00:00

  • HYDROLYSIS OF PROTEINS AND NUCLEIC ACIDS BY LANCEFIELD GROUP A AND OTHER STREPTOCOCCI.

    abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.). Hydrolysis of proteins and nucleic acids by Lancefield group A and other streptococci. J. Bacteriol. 86:1270-1274. 1963.-Classically, group A streptococci have been considered to be nonproteolytic in spite of the observation that a proteinase is produc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.6.1270-1274.1963

    authors: DEIBEL RH

    更新日期:1963-12-01 00:00:00

  • Periplasmic nitrate reductase (NapABC enzyme) supports anaerobic respiration by Escherichia coli K-12.

    abstract::Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.5.1314-1323.2002

    authors: Stewart V,Lu Y,Darwin AJ

    更新日期:2002-03-01 00:00:00

  • Staphylococcal penicillinase. I. Inhibition and stimulation of activity.

    abstract::Saz, Arthur K. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), Dolores L. Lowery, and Leah J. Jackson. Staphylococcal penicillinase. I. Inhibition and stimulation of activity. J. Bacteriol. 82:298-304. 1961.-The penicillinase extracted from a penicillin-resistant strain of Staphylococcus aureus...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.2.298-304.1961

    authors: SAZ AK,LOWERY DL,JACKSON LJ

    更新日期:1961-08-01 00:00:00

  • DETECTION AND PERSISTENCE OF VI ANTIGEN IN TISSUES OF ACTIVELY IMMUNIZED MICE.

    abstract::Gaines, Sidney (Walter Reed Army Institute of Research, Washington, D.C.), Julius A. Currie, and Joseph G. Tully. Detection and persistence of Vi antigen in tissues of actively immunized mice. J. Bacteriol. 89:776-781. 1965.-The presence, distribution, and persistence of Vi antigen in mouse tissue was determined by me...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.776-781.1965

    authors: GAINES S,CURRIE JA,TULLY JG

    更新日期:1965-03-01 00:00:00

  • Mechanism of light-dependent proton translocation by bacteriorhodopsin.

    abstract::Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/jb.175.6.1555-1560.1993

    authors: Krebs MP,Khorana HG

    更新日期:1993-03-01 00:00:00

  • Role of premature stop codons in bacterial evolution.

    abstract::When the stop codons TGA, TAA, and TAG are found in the second and third reading frames of a protein-encoding gene, they are considered premature stop codons (PSC). Deinococcus radiodurans disproportionately favored TGA more than the other two triplets as a PSC. The TGA triplet was also found more often in noncoding r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00682-08

    authors: Wong TY,Fernandes S,Sankhon N,Leong PP,Kuo J,Liu JK

    更新日期:2008-10-01 00:00:00

  • Characterization of EvgAS-YdeO-GadE branched regulatory circuit governing glutamate-dependent acid resistance in Escherichia coli.

    abstract::Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.21.7378-7389.2004

    authors: Ma Z,Masuda N,Foster JW

    更新日期:2004-11-01 00:00:00

  • Growth of staphylococci on mercuric chloride agar.

    abstract::Smith, P. B. (Communicable Disease Center, Atlanta, Ga.). Growth of staphylococci on mercuric chloride agar. J. Bacteriol. 84:1016-1019. 1962.-An attempt has been made to corroborate a report that "epidemic" strains of staphylococci are more resistant to mercuric salts than are "nonepidemic" strains. A comparison of m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.5.1016-1019.1962

    authors: SMITH PB

    更新日期:1962-11-01 00:00:00

  • Alcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase.

    abstract::The Alcaligenes eutrophus JMP134 tfdA gene, encoding the enzyme responsible for the first step in 2,4-dichlorophenoxyacetic acid (2,4-D) biodegradation, was overexpressed in Escherichia coli, and several enzymatic properties of the partially purified gene product were examined. Although the tfdA-encoded enzyme is typi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.2083-2086.1993

    authors: Fukumori F,Hausinger RP

    更新日期:1993-04-01 00:00:00

  • The periplasmic protein MppA requires an additional mutated locus to repress marA expression in Escherichia coli.

    abstract::Escherichia coli strain TP985, which has an insertional mutation in the gene for the periplasmic murein tripeptide binding protein MppA, was previously reported to overproduce MarA and exhibit a multiple-antibiotic resistance (Mar) phenotype (H. Li and J. T. Park, J. Bacteriol. 181:4842-4847, 1999). We found that TP98...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.4.1465-1469.2003

    authors: Bina X,Perreten V,Levy SB

    更新日期:2003-02-01 00:00:00

  • HK022 Nun Requires Arginine-Rich Motif Residues Distinct from λ N.

    abstract:UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00466-15

    authors: Tawk CS,Ghattas IR,Smith CA

    更新日期:2015-11-01 00:00:00

  • Overproduction of tryptophanyl-tRNA synthetase relieves transcription termination at the Escherichia coli tryptophan operon attenuator.

    abstract::Overproduction of tryptophanyl-tRNA synthetase increased trp operon expression by reducing transcription termination at the trp attenuator. The total cellular level of charged tRNATrp was not affected by increased levels of the synthetase. We propose that excess synthetase binds charged tRNATrp and reduces the concent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.2.805-807.1984

    authors: Das A,Yanofsky C

    更新日期:1984-11-01 00:00:00

  • SOME CYTOLOGICAL AND PATHOGENIC PROPERTIES OF SPHEROPLASTS OF CANDIDA ALBICANS.

    abstract::Kobayashi, George S. (Tulane University, New Orleans, La.), Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts of Candida albicans. J. Bacteriol. 88:795-801. 1964.-Spheroplasts of Candida albicans were prepared by use of an enzymatic mixture from the digestive tract of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.3.795-801.1964

    authors: KOBAYASHI GS,FRIEDMAN L,KOFROTH JF

    更新日期:1964-09-01 00:00:00

  • DNA primase of plasmid ColIb is involved in conjugal DnA synthesis in donor and recipient bacteria.

    abstract::The sog gene of the IncI alpha group plasmid ColIb is known to encode a DNA primase that can substitute for defective host primase in dnaG mutants of Escherichia coli during discontinuous DNA replication. The biological significance of this enzyme was investigated by using sog mutants, constructed from a derivative of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Chatfield LK,Orr E,Boulnois GJ,Wilkins BM

    更新日期:1982-12-01 00:00:00

  • Global control in Salmonella typhimurium: two-dimensional electrophoretic analysis of starvation-, anaerobiosis-, and heat shock-inducible proteins.

    abstract::The response of Salmonella typhimurium to various forms of environmental stress was examined by using O'Farrell two-dimensional gel electrophoresis. Polypeptides (a total of 110) which quantitatively increased during various starvations, anaerobiosis, or heat shock were identified and cataloged in reference to a stand...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.1.420-424.1986

    authors: Spector MP,Aliabadi Z,Gonzalez T,Foster JW

    更新日期:1986-10-01 00:00:00

  • Mapping of the tryptophan genes of Acinetobacter calcoaceticus by transformation.

    abstract::Auxotrophs of Acinetobacter calcoaceticus blocked in each reaction of the synthetic pathway from chorismic acid to tryptophan were obtained after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. One novel class was found to be blocked in both anthranilate and p-aminobenzoate synthesis; these mutants (trpG) require p-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.797-805.1972

    authors: Sawula RV,Crawford IP

    更新日期:1972-11-01 00:00:00

  • Deoxyribonucleic acid relatedness among species of Erwinia and between Erwinia species and other enterobacteria.

    abstract::Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.1.12-17.1972

    authors: Brenner DJ,Fanning GR,Steigerwalt AG

    更新日期:1972-04-01 00:00:00

  • Outer membrane of gram-negative bacteria. XVIII. Electron microscopic studies on porin insertion sites and growth of cell surface of Salmonella typhimurium.

    abstract::Salmonella typhimurium contains three "major proteins" or "porins" (34K, 35K, and 36K) in the outer membrane. A mutant strain producing only the 35K porin was first grown in media containing high concentrations of NaCl to "repress" the porin synthesis and then was shifted into a medium without NaCl. The newly made por...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.2.687-702.1978

    authors: Smit J,Nikaido H

    更新日期:1978-08-01 00:00:00

  • The thioredoxin system of Penicillium chrysogenum and its possible role in penicillin biosynthesis.

    abstract::Penicillium chrysogenum is an important producer of penicillin antibiotics. A key step in their biosynthesis is the oxidative cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N by the enzyme isopenicillin N synthase (IPNS). bis-ACV, the oxidized disulfide form of ACV is, however, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.4.973-984.1994

    authors: Cohen G,Argaman A,Schreiber R,Mislovati M,Aharonowitz Y

    更新日期:1994-02-01 00:00:00

  • Reconstitution of signaling in bacterial chemotaxis.

    abstract::Strains missing several genes required for chemotaxis toward amino acids, peptides, and certain sugars were tethered and their rotational behavior was analyzed. Null strains (called gutted) were deleted for genes that code for the transducers Tsr, Tar, Tap, and Trg and for the cytoplasmic proteins CheA, CheW, CheR, Ch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.1878-1885.1987

    authors: Wolfe AJ,Conley MP,Kramer TJ,Berg HC

    更新日期:1987-05-01 00:00:00

  • Draft genome sequence of Bizionia argentinensis, isolated from Antarctic surface water.

    abstract::A psychrotolerant marine bacterial strain, designated JUB59(T), was isolated from Antarctic surface seawater and classified as a new species of the genus Bizionia. Here, we present the first draft genome sequence for this genus, which suggests interesting features such as UV resistance, hydrolytic exoenzymes, and nitr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06245-11

    authors: Lanzarotti E,Pellizza L,Bercovich A,Foti M,Coria SH,Vazquez SC,Ruberto L,Hernández EA,Dias RL,Mac Cormack WP,Cicero DO,Smal C,Nicolas MF,Vasconcelos AT,Marti MA,Turjanski AG

    更新日期:2011-12-01 00:00:00

  • Purification and characterization of thin pili of IncI1 plasmids ColIb-P9 and R64: formation of PilV-specific cell aggregates by type IV pili.

    abstract::Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.11.2842-2848.1998

    authors: Yoshida T,Furuya N,Ishikura M,Isobe T,Haino-Fukushima K,Ogawa T,Komano T

    更新日期:1998-06-01 00:00:00

  • Genetic requirements for potassium ion-dependent colony spreading in Bacillus subtilis.

    abstract::Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.24.8462-8469.2005

    authors: Kinsinger RF,Kearns DB,Hale M,Fall R

    更新日期:2005-12-01 00:00:00

  • Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.

    abstract::Thiamine pyrophosphate (TPP) is a required cofactor in Salmonella typhimurium that is generated de novo by the condensation of 4-amino-5-hydroxymethyl pyrimidine (HMP) pyrophosphate and 4-methyl-5-(beta-hydroxyethyl)-thiazole (THZ) monophosphate. The THZ and HMP moieties are independently synthesized, and labeling stu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.13.4399-4402.1997

    authors: Webb E,Claas K,Downs DM

    更新日期:1997-07-01 00:00:00

  • Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase.

    abstract::A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identifi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5968-5979.1990

    authors: Crouzet J,Cauchois L,Blanche F,Debussche L,Thibaut D,Rouyez MC,Rigault S,Mayaux JF,Cameron B

    更新日期:1990-10-01 00:00:00

  • Tryptophan operon regulation in interspecific hybrids of enteric bacteria.

    abstract::We examined tryptophan regulation in merodiploid hybrids in which a plasmid carrying the trp operon of Escherichia was introduced into Trp mutants of other enteric genera, or in which a plasmid carrying the trpR+ (repressor) gene of E. coli was transfered into fully constitutive trpR mutants of other genera. In these ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.2.679-689.1976

    authors: Manson MD,Yanofsky C

    更新日期:1976-05-01 00:00:00