Abstract:
:Periplasmic nitrate reductase (NapABC enzyme) has been characterized from a variety of proteobacteria, especially Paracoccus pantotrophus. Whole-genome sequencing of Escherichia coli revealed the structural genes napFDAGHBC, which encode NapABC enzyme and associated electron transfer components. E. coli also expresses two membrane-bound proton-translocating nitrate reductases, encoded by the narGHJI and narZYWV operons. We measured reduced viologen-dependent nitrate reductase activity in a series of strains with combinations of nar and nap null alleles. The napF operon-encoded nitrate reductase activity was not sensitive to azide, as shown previously for the P. pantotrophus NapA enzyme. A strain carrying null alleles of narG and narZ grew exponentially on glycerol with nitrate as the respiratory oxidant (anaerobic respiration), whereas a strain also carrying a null allele of napA did not. By contrast, the presence of napA+ had no influence on the more rapid growth of narG+ strains. These results indicate that periplasmic nitrate reductase, like fumarate reductase, can function in anaerobic respiration but does not constitute a site for generating proton motive force. The time course of phi(napF-lacZ) expression during growth in batch culture displayed a complex pattern in response to the dynamic nitrate/nitrite ratio. Our results are consistent with the observation that phi(napF-lacZ) is expressed preferentially at relatively low nitrate concentrations in continuous cultures (H. Wang, C.-P. Tseng, and R. P. Gunsalus, J. Bacteriol. 181:5303-5308, 1999). This finding and other considerations support the hypothesis that NapABC enzyme may function in E. coli when low nitrate concentrations limit the bioenergetic efficiency of nitrate respiration via NarGHI enzyme.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Stewart V,Lu Y,Darwin AJdoi
10.1128/jb.184.5.1314-1323.2002keywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
1314-23issue
5eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::A method of assay of immunogenic potency of the cholera gut toxin is described; it is based on the relation of dose of antigen to neutralizing antibody titer produced in the rabbit under defined conditions and allows quantification of immunogenicity as immunogenic units per milligram of protein. Evidence, based on imm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.2.985-993.1969
更新日期:1969-11-01 00:00:00
abstract::Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.23.6952-6957.1995
更新日期:1995-12-01 00:00:00
abstract::A tandem pair of nearly identical genes from Serpulina hyodysenteriae (B204) were cloned and sequenced. The full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kDa extracytoplasmic protein p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.2.444-448.1998
更新日期:1998-01-01 00:00:00
abstract::Bulen, William A. (Charles F. Kettering Foundation, Yellow Springs, Ohio). Effect of tungstate on the uptake and function of molybdate in Azotobacter agilis. J. Bacteriol. 82:130-134. 1961.-The reported competitive inhibition of molybdate by tungstate was investigated in an effort to elucidate molybdenum functions ass...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.1.130-134.1961
更新日期:1961-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.24.7180-7186.1996
更新日期:1996-12-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.01104-13
更新日期:2014-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.9.2797-2801.1999
更新日期:1999-05-01 00:00:00
abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.6959-6964.1997
更新日期:1997-11-01 00:00:00
abstract::Nahmias, André J. (Boston University School of Medicine, Boston, Mass.), and Sidney Kibrick. Inhibitory effect of heparin on herpes simplex virus. J. Bacteriol. 87:1060-1066. 1964.-A substance inhibitory to herpes simplex virus was observed during experiments with leukocyte cultures. The component in the cultures resp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1060-1066.1964
更新日期:1964-05-01 00:00:00
abstract::We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.1197-1200.1978
更新日期:1978-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.359-369.1962
更新日期:1962-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.8.2665-2672.1991
更新日期:1991-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.3.1380-1386.1974
更新日期:1974-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.558-561.1975
更新日期:1975-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.23.6960-6967.1996
更新日期:1996-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2432-2439.1987
更新日期:1987-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01579-14
更新日期:2014-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.156-162.1996
更新日期:1996-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.193-200.1979
更新日期:1979-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.160.1.175-183.1984
更新日期:1984-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.96-104.1985
更新日期:1985-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.2.646-649.1992
更新日期:1992-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3536-3543.1994
更新日期:1994-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02107-12
更新日期:2013-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01552-07
更新日期:2008-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.1.57-61.1981
更新日期:1981-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1005-1011.1984
更新日期:1984-06-01 00:00:00
abstract::In Bacillus subtilis, carbon catabolite repression (CCR) of many genes is mediated at cis-acting carbon repression elements (cre) by the catabolite repressor protein CcpA. Mutations in transcription-repair coupling factor (mfd) partially relieve CCR at cre sites located downstream of transcriptional start sites by abo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.24.6649-6654.1998
更新日期:1998-12-01 00:00:00
abstract::The primary sequence of the small cysteine-rich protein (EnvA) of Chlamydia psittaci 6BC has been shown to possess a potential lipid modification/signal peptidase II-processing site, and the mature protein was labeled by a [3H]palmitic acid precursor. We further characterized the mature EnvA, showing that it lacks the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.6082-6087.1994
更新日期:1994-10-01 00:00:00
abstract::The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fun...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.43-46.1968
更新日期:1968-01-01 00:00:00