MECHANISM OF ACTION OF THE TOXIN OF BACILLUS ANTHRACIS II. : Alkaline Phosphatasemia Produced by Culture Filtrates of Various Bacilli.

abstract：:Cryptococcus neoformans is an important pathogenic fungus that has been classified as a basidiomycete. Little is known of the molecular genetics of this fungal pathogen. To begin such studies, we devised a procedure for extraction of DNA from cryptococci; this method involved the use of the cell wall-active enzyme Nov...

journal_title：Journal of bacteriology

authors： Restrepo BI,Barbour AG

更新日期：1989-10-01 00:00:00

abstract：:Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

journal_title：Journal of bacteriology

authors： Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

更新日期：1995-11-01 00:00:00

abstract：:Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...

journal_title：Journal of bacteriology

authors： Silbert DF,Ruch F,Vagelos PR

更新日期：1968-05-01 00:00:00

abstract：:The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...

journal_title：Journal of bacteriology

authors： Osterås M,Driscoll BT,Finan TM

更新日期：1995-03-01 00:00:00

abstract：:A gene coding for thermophilic beta-amylase of Clostridium thermosulfurogenes was cloned into Bacillus subtilis, and its nucleotide sequence was determined. The nucleotide sequence suggested that the thermophilic beta-amylase is translated from monocistronic mRNA as a secretory precursor with a signal peptide of 32 am...

journal_title：Journal of bacteriology

authors： Kitamoto N,Yamagata H,Kato T,Tsukagoshi N,Udaka S

更新日期：1988-12-01 00:00:00

abstract：:Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separa...

journal_title：Journal of bacteriology

authors： Waller LN,Stump MJ,Fox KF,Harley WM,Fox A,Stewart GC,Shahgholi M

更新日期：2005-07-01 00:00:00

abstract：:A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmI...

journal_title：Journal of bacteriology

authors： Decker H,Hutchinson CR

更新日期：1993-06-01 00:00:00

abstract：:Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization ...

journal_title：Journal of bacteriology

authors： Dietrich SM

更新日期：1976-09-01 00:00:00

abstract：:In order to study the Salmonella typhimurium cobalamin biosynthetic pathway, the S. typhimurium cob operon was isolated and cloned into Escherichia coli. This approach has given the new host of the cob operon the ability to make cobalamins de novo, an ability that had probably been lost by this organism. In total, 20 ...

journal_title：Journal of bacteriology

authors： Raux E,Lanois A,Levillayer F,Warren MJ,Brody E,Rambach A,Thermes C

更新日期：1996-02-01 00:00:00

abstract：:We have used transcriptional and translational fusions between various vir gene promoters and the lacZ gene to study the regulation of vir genes. Like other vir promoters, the virA promoter was induced by acetosyringone in a virA virG-dependent fashion. In addition to being induced by acetosyringone, the virG promoter...

journal_title：Journal of bacteriology

authors： Winans SC,Kerstetter RA,Nester EW

更新日期：1988-09-01 00:00:00

abstract：:The activities of phosphofructokinase (PFK), fructose diphosphatase (FDP), nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP)-linked isocitrate dehydrogenases (IDHNAD, IDHNADP), two NAD-linked glutamate dehydrogenases (GDH1, GDH2), and isocitrate lyase were studied during the development of the two pheno...

journal_title：Journal of bacteriology

authors： Ingebretsen OC,Sanner T

更新日期：1976-06-01 00:00:00

abstract：:In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potent...

journal_title：Journal of bacteriology

authors： Salema M,Lolkema JS,San Romão MV,Lourero Dias MC

更新日期：1996-06-01 00:00:00

abstract：:Using a number of approaches we were unable to demonstrate a chemotactic response of Myxococcus xanthus to a variety of defined and complex materials. These data in addition to a number of prima facie arguments considerably reduce the likelihood that M. xanthus possesses a mechanism for chemotactic behavior. ...

journal_title：Journal of bacteriology

authors： Dworkin M,Eide D

更新日期：1983-04-01 00:00:00

abstract：:A genetic system was developed to investigate the supercoil structure of bacterial chromosomes. New res-carrying transposons were derived from MudI1734 (MudJr1 and MudJr2) and Tn10 (Tn10dGn). The MudJr1 and MudJr2 elements each have a res site in opposite orientation so that when paired with a Tn10dGn element in the s...

journal_title：Journal of bacteriology

authors： Higgins NP,Yang X,Fu Q,Roth JR

更新日期：1996-05-01 00:00:00

abstract：:Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine. oleY codes for a methyltransferase involved in the biosynthesis of L-oleandrose. This gene was overexpressed in Escherichia coli to form inclusion bodies and in Strept...

journal_title：Journal of bacteriology

authors： Rodríguez L,Rodríguez D,Olano C,Braña AF,Méndez C,Salas JA

更新日期：2001-09-01 00:00:00

abstract：:Amber mutants of bacteriophage P1 were used to identify functions involved in late regulation of the P1 lytic growth cycle. A single function has been genetically identified to be involved in activation of the phage-specific late promoter sequence Ps. In vivo, P1 gene 10 amber mutants fail to trans activate a lacZ ope...

journal_title：Journal of bacteriology

authors： Lehnherr H,Guidolin A,Arber W

更新日期：1991-10-01 00:00:00

abstract：:The exopolysaccharide of Pseudomonas marginalis HT041B has been characterized as a 1,3-linked galactoglucan in which galactose and glucose are in the alpha- and beta-anomeric configurations, respectively. The polysaccharide is substituted with pyruvate at the 4 and 6 positions of galactose and with succinic acid at ei...

journal_title：Journal of bacteriology

authors： Osman SF,Fett WF

更新日期：1989-03-01 00:00:00

abstract：:Mycoplasma fermentans is a microorganism commonly found in the genitourinary and respiratory tracts of healthy individuals and AIDS patients. The complete genome of the repetitive-sequence-rich M. fermentans strain M64 is reported here. Comparative genomics analysis revealed dramatic differences in genome size between...

journal_title：Journal of bacteriology

authors： Shu HW,Liu TT,Chan HI,Liu YM,Wu KM,Shu HY,Tsai SF,Hsiao KJ,Hu WS,Ng WV

更新日期：2011-08-01 00:00:00

abstract：:Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that th...

journal_title：Journal of bacteriology

authors： MacPhee KG,Nelson RE,Schuster SM

更新日期：1983-10-01 00:00:00

abstract：:In Salmonella, ilv-linked rfe genes participate in the biosynthesis of the enterobacterial common antigen (CA) as well as of certain types of O antigen (serogroups C1 and L). rff genes, probably in the same cluster with rfe, are required for CA synthesis (P.H. Mäkelä et al., in preparation). Several Escherichia coli s...

journal_title：Journal of bacteriology

authors： Schmidt G,Mayer H,Mäkelä PH

更新日期：1976-08-01 00:00:00

abstract：:Small cytoplasmic RNA (scRNA; 271 nucleotides) is an abundant and stable RNA of the gram-positive bacterium Bacillus subtilis. To investigate the function of scRNA in B. subtilis cells, we developed a strain that is dependent on isopropyl-beta-D-thiogalactopyranoside for scRNA synthesis by fusing the chromosomal scr l...

journal_title：Journal of bacteriology

authors： Nakamura K,Imai Y,Nakamura A,Yamane K

更新日期：1992-04-01 00:00:00

abstract：:Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...

journal_title：Journal of bacteriology

authors： Zagotta MT,Wilson DB

更新日期：1990-02-01 00:00:00

abstract：:Developmental expression of the Myxococcus xanthus gene 4521 requires extracellular A-signal. This signal is generated in response to nutrient limitation and functions in cell density sensing. To identify the upstream limit of the minimum region required in vivo for A-signal-dependent 4521 expression, a 5' deletion an...

journal_title：Journal of bacteriology

authors： Gulati P,Xu D,Kaplan HB

更新日期：1995-08-01 00:00:00

abstract：:Saccharomyces cerevisiae degrades allantoin in five steps to ammonia, CO(2), and glyoxylate. Previously we demonstrated that allophanic acid, the last intermediate of the pathway, was required for induction of all five degradative enzymes. The data presented here indicate that oxaluric acid, an allophanate analogue, i...

journal_title：Journal of bacteriology

authors： Sumrada R,Cooper TG

更新日期：1974-03-01 00:00:00

abstract：:Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretio...

journal_title：Journal of bacteriology

authors： Hodges LD,Lee LY,McNett H,Gelvin SB,Ream W

更新日期：2009-01-01 00:00:00

abstract：:The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...

journal_title：Journal of bacteriology

authors： Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

更新日期：1984-01-01 00:00:00

abstract：:Colicinogenic factors ColI and ColV, which have been shown to behave as sex factors, could not be induced with mitomycin C. In contrast, the ColE(1), ColE(2), and ColE(3) factors, which do not exhibit any fertility factor characteristics, are inducible by this agent. The induced production of colicins E(1), E(2), and ...

journal_title：Journal of bacteriology

authors： Herschman HR,Helinski DR

更新日期：1967-09-01 00:00:00

abstract：:Protoanemonin is a toxic metabolite which may be formed during the degradation of some chloroaromatic compounds, such as polychlorinated biphenyls, by natural microbial consortia. We show here that protoanemonin can be transformed by dienelactone hydrolase of Pseudomonas sp. strain B13 to cis-acetylacrylate. Although ...

journal_title：Journal of bacteriology

authors： Brückmann M,Blasco R,Timmis KN,Pieper DH

更新日期：1998-01-01 00:00:00

abstract：:The RNA polymerase sigma subunit, sigmaH, of Bacillus subtilis is required for the transcription of genes that are induced in late-growth cultures at high cell density, including genes that function in sporulation. The expression of sigmaH-controlled genes is repressed when nutrient broth sporulation medium (Difco spo...

journal_title：Journal of bacteriology

authors： Cosby WM,Zuber P

更新日期：1997-11-01 00:00:00

abstract：:In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...

journal_title：Journal of bacteriology

authors： Hsieh FC,Chen CT,Weng YT,Peng SS,Chen YC,Huang LY,Hu HT,Wu YL,Lin NC,Wu WF

更新日期：2011-10-01 00:00:00