Nucleotide sequence of the papA gene encoding the Pap pilus subunit of human uropathogenic Escherichia coli.

Abstract:

:The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polypeptide secondary structure of the Pap pili subunit.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

doi

10.1128/JB.157.1.330-333.1984

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

330-3

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

157

pub_type

杂志文章
  • Biochemical and phylogenetic characterization of a novel diaminopimelate biosynthesis pathway in prokaryotes identifies a diverged form of LL-diaminopimelate aminotransferase.

    abstract::A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an LL-DAP aminotransferase (DapL, EC 2.6.1.83) to catalyze the direct conversion of L-2,3,4,5-tetrahydrodipicolinate to LL-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01381-07

    authors: Hudson AO,Gilvarg C,Leustek T

    更新日期:2008-05-01 00:00:00

  • The Bacillus subtilis tyrZ gene encodes a highly selective tyrosyl-tRNA synthetase and is regulated by a MarR regulator and T box riboswitch.

    abstract:UNLABELLED:Misincorporation of D-tyrosine (D-Tyr) into cellular proteins due to mischarging of tRNA(Tyr) with D-Tyr by tyrosyl-tRNA synthetase inhibits growth and biofilm formation of Bacillus subtilis. Furthermore, many B. subtilis strains lack a functional gene encoding D-aminoacyl-tRNA deacylase, which prevents misi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00008-15

    authors: Williams-Wagner RN,Grundy FJ,Raina M,Ibba M,Henkin TM

    更新日期:2015-05-01 00:00:00

  • Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.

    abstract::Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1556-1562.1977

    authors: Currier TC,Haury JF,Wolk CP

    更新日期:1977-03-01 00:00:00

  • Conjugative plasmids in Neisseria gonorrhoeae.

    abstract::A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.1.278-286.1978

    authors: Sox TE,Mohammed W,Blackman E,Biswas G,Sparling PF

    更新日期:1978-04-01 00:00:00

  • DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication.

    abstract::The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.2148-2150.2001

    authors: Ishigo-Oka D,Ogasawara N,Moriya S

    更新日期:2001-03-01 00:00:00

  • The lrp gene product regulates expression of lysU in Escherichia coli K-12.

    abstract::In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.9.2779-2784.1992

    authors: Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

    更新日期:1992-05-01 00:00:00

  • The LysR-type transcriptional regulator QseD alters type three secretion in enterohemorrhagic Escherichia coli and motility in K-12 Escherichia coli.

    abstract::Enterohemorrhagic Escherichia coli (EHEC) O157:H7 responds to the host-produced epinephrine and norepinephrine, and bacterially produced autoinducer 3 (AI-3), through two-component systems. Further integration of multiple regulatory signaling networks, involving regulators such as the LysR-type transcriptional regulat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00382-10

    authors: Habdas BJ,Smart J,Kaper JB,Sperandio V

    更新日期:2010-07-01 00:00:00

  • TK1211 Encodes an Amino Acid Racemase towards Leucine and Methionine in the Hyperthermophilic Archaeon Thermococcus kodakarensis.

    abstract::Members of Thermococcales harbor a number of genes encoding putative aminotransferase Class III enzymes. Here, we characterized the TK1211 protein from the hyperthermophilic archaeon Thermococcus kodakarensis The TK1211 gene was expressed in T. kodakarensis under the control of the strong, constitutive promoter of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00617-20

    authors: Zheng RC,Lu XF,Tomita H,Hachisuka SI,Zheng YG,Atomi H

    更新日期:2021-01-19 00:00:00

  • Enteropathogenic Escherichia coli: identification of a gene cluster coding for bundle-forming pilus morphogenesis.

    abstract::Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.9.2613-2628.1996

    authors: Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK

    更新日期:1996-05-01 00:00:00

  • Modulation of Response Regulator CheY Reaction Kinetics by Two Variable Residues That Affect Conformation.

    abstract::Microorganisms and plants utilize two-component systems to regulate adaptive responses to changing environmental conditions. Sensor kinases detect stimuli and alter their autophosphorylation activity accordingly. Signal propagation occurs via the transfer of phosphoryl groups from upstream kinases to downstream respon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00089-20

    authors: Straughn PB,Vass LR,Yuan C,Kennedy EN,Foster CA,Bourret RB

    更新日期:2020-07-09 00:00:00

  • Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes.

    abstract::The nucleotide sequences of the Rhodobacter sphaeroides hemA and hemT genes, encoding 5-aminolevulinic acid (ALA) synthase isozymes, were determined. ALA synthase catalyzes the condensation of glycine and succinyl coenzyme A, the first and rate-limiting step in tetrapyrrole biosynthesis. The hemA and hemT structural g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.8.2292-2303.1993

    authors: Neidle EL,Kaplan S

    更新日期:1993-04-01 00:00:00

  • Transcriptional analysis and mutation of a dnaA-like gene in Synechocystis sp. strain PCC 6803.

    abstract::Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.18.4946-4949.1998

    authors: Richter S,Hagemann M,Messer W

    更新日期:1998-09-01 00:00:00

  • Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli.

    abstract::DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2569-2575.1991

    authors: Washburn BK,Kushner SR

    更新日期:1991-04-01 00:00:00

  • INTRACELLULAR BEHAVIOR OF HISTOPLASMA CAPSULATUM.

    abstract::Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.33-38.1964

    authors: HOWARD DH

    更新日期:1964-01-01 00:00:00

  • Disruption of VirB6 Paralogs in Anaplasma phagocytophilum Attenuates Its Growth.

    abstract::Many pathogenic bacteria translocate virulence factors into their eukaryotic hosts by means of type IV secretion systems (T4SS) spanning the inner and outer membranes. Genes encoding components of these systems have been identified within the order Rickettsiales based upon their sequence similarities to other prototyp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00301-20

    authors: Crosby FL,Munderloh UG,Nelson CM,Herron MJ,Lundgren AM,Xiao YP,Allred DR,Barbet AF

    更新日期:2020-11-04 00:00:00

  • Bioinformatic and biochemical evidence for the identification of the type III secretion system needle protein of Chlamydia trachomatis.

    abstract::Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01671-07

    authors: Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

    更新日期:2008-03-01 00:00:00

  • Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity.

    abstract::The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.8.2920-2925.2005

    authors: Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

    更新日期:2005-04-01 00:00:00

  • Differential Reduction of Tellurite by Growing Colonies of Normal Yeast and Respiration-Deficient Mutants.

    abstract::Nagai, Susumu (National Women's University, Nara, Japan). Differential reduction of tellurite by growing colonies of normal yeast and respiration-deficient mutants. J. Bacteriol. 90:220-222. 1965.-A differential reduction of sodium tellurite was observed between normal and respiration-deficient mutant colonies of seve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.1.220-222.1965

    authors: Nagai S

    更新日期:1965-07-01 00:00:00

  • RNase III processing of intervening sequences found in helix 9 of 23S rRNA in the alpha subclass of Proteobacteria.

    abstract::We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.17.4719-4729.2000

    authors: Evguenieva-Hackenberg E,Klug G

    更新日期:2000-09-01 00:00:00

  • Multiple determinants of functional mRNA stability: sequence alterations at either end of the lacZ gene affect the rate of mRNA inactivation.

    abstract::The Escherichia coli lacZ gene was used as a model system to identify specific sequence elements affecting mRNA stability. Various insertions and substitutions at the ribosome-binding site increased or decreased the rate of mRNA inactivation by up to fourfold. Deletion of a dyad symmetry, which may give rise to a very...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.7.2167-2172.1991

    authors: Petersen C

    更新日期:1991-04-01 00:00:00

  • Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids.

    abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.153-160.1979

    authors: Emerich DW,Ruiz-Argüeso T,Ching TM,Evans HJ

    更新日期:1979-01-01 00:00:00

  • Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12.

    abstract::Two sensor proteins, NarX and NarQ, mediate nitrate regulation of anaerobic respiratory gene expression. Either of these sensors is sufficient to signal the presence of nitrate to the response regulator protein, NarL, a transcriptional activator and repressor. Two observations suggested the existence of a second respo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.11.3259-3268.1993

    authors: Rabin RS,Stewart V

    更新日期:1993-06-01 00:00:00

  • Lethality of the covalent linkage between mislocalized major outer membrane lipoprotein and the peptidoglycan of Escherichia coli.

    abstract::The major outer membrane lipoprotein (Lpp) of Escherichia coli possesses serine at position 2, which is thought to function as the outer membrane sorting signal, and lysine at the C terminus, through which Lpp covalently associates with peptidoglycan. Arginine (R) is present before the C-terminal lysine in the wild-ty...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.9.2857-2862.1997

    authors: Yakushi T,Tajima T,Matsuyama S,Tokuda H

    更新日期:1997-05-01 00:00:00

  • Mapping and cloning of gldA, the structural gene of the Escherichia coli glycerol dehydrogenase.

    abstract::gldA, the structural gene for the NAD(+)-dependent glycerol dehydrogenase, was mapped at 89.2 min on the Escherichia coli linkage map, cotransducible with, but not adjacent to, the glpFKX operon encoding the proteins for the uptake and phosphorylation of glycerol. gldA was cloned, and its position on the physical map ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.6.1796-1800.1994

    authors: Truniger V,Boos W

    更新日期:1994-03-01 00:00:00

  • SUPPRESSION OF ROUS SARCOMA VIRUS GROWTH IN TISSUE CULTURES BY MYCOPLASMA ORALE.

    abstract::Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.2.534-540.1965

    authors: SOMERSON NL,COOK MK

    更新日期:1965-08-01 00:00:00

  • Assembly site of bacteriophage f1 corresponds to adhesion zones between the inner and outer membranes of the host cell.

    abstract::Morphogenesis of the filamentous bacteriophage f1 occurred at adhesion zones between the inner and outer membranes of the host cell. Quantitation of adhesion zones in cells infected with mutant phage strains suggested that the phage gene I protein may be involved in the formation of adhesion zones for phage assembly. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.3.1270-1274.1985

    authors: Lopez J,Webster RE

    更新日期:1985-09-01 00:00:00

  • Regulation of a macrolide resistance-beta-galactosidase (ermC-lacZ) gene fusion in Escherichia coli.

    abstract::A fusion constructed between the putative attenuator plus the first 219 nucleotides of the ermC (erythromycin resistance) structural gene and a 5' terminally deleted lacZ gene produced a moderate, basal level of beta-galactosidase which was increased by erythromycin addition. Another construction containing an intact ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.381-384.1984

    authors: Kirsch DR,Lai MH

    更新日期:1984-07-01 00:00:00

  • Suppressor analysis of mutations in the loop 2-3 motif of lactose permease: evidence that glycine-64 is an important residue for conformational changes.

    abstract::A superfamily of transport proteins, which includes the lactose permease of Escherichia coli, contains a highly conserved motif, G-X-X-X-D/E-R/K-X-G-R/K-R/K, in the loops that connect transmembrane segments 2 and 3 and transmembrane segments 8 and 9. Previous analysis of this motif in the lactose permease (A. E. Jesse...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.8.2616-2622.1997

    authors: Jessen-Marshall AE,Parker NJ,Brooker RJ

    更新日期:1997-04-01 00:00:00

  • Cloning of the ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) structural genes from Salmonella typhimurium LT2.

    abstract::The structural genes of ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) from Salmonella typhimurium LT2 were cloned on a 5.8-kilobase-pair insert in the SalI site of pBR322. A single strand specific radioactive probe containing the N terminus of the Escherichia coli K-12 glgC gene in M13mp8 was used t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4349-4354.1987

    authors: Leung PS,Preiss J

    更新日期:1987-09-01 00:00:00

  • Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei.

    abstract::Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.4.925-930.1966

    authors: Birnbaum J,Lichstein HC

    更新日期:1966-10-01 00:00:00