Enteropathogenic Escherichia coli: identification of a gene cluster coding for bundle-forming pilus morphogenesis.


:Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at least 12 open reading frames, including bfpA, that could direct the synthesis of a morphologically normal BFP filament. Within the bfp gene cluster, we identified open reading frames that share homology with other type IV pilus accessory genes and with genes required for transformation competence and protein secretion. Immediately upstream of the bfp gene cluster, we identified a potential replication origin including genes that are predicted to encode proteins homologous with replicase and resolvase. Restriction fragment length polymorphism analysis of DNA from six additional EPEC serotypes showed that the organization of the bfp gene cluster and its juxtaposition with a potential plasmid origin of replication are highly conserved features of the EPEC biotype.


J Bacteriol


Journal of bacteriology


Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK




Has Abstract


1996-05-01 00:00:00












  • ExoS controls the cell contact-mediated switch to effector secretion in Pseudomonas aeruginosa.

    abstract::Type III secretion is used by many gram-negative bacterial pathogens to directly deliver protein toxins (effectors) into targeted host cells. In all cases, secretion of effectors is triggered by host cell contact, although the mechanism is unclear. In Pseudomonas aeruginosa, expression of all type III secretion-relate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cisz M,Lee PC,Rietsch A

    更新日期:2008-04-01 00:00:00

  • YkdA and YvtA, HtrA-like serine proteases in Bacillus subtilis, engage in negative autoregulation and reciprocal cross-regulation of ykdA and yvtA gene expression.

    abstract::HtrA-type serine proteases participate in folding and degradation of aberrant proteins and in processing and maturation of native proteins. Mutation of the corresponding genes often confers a pleiotropic phenotype that can include temperature sensitivity, sensitivity to osmotic and oxidative stress, and attenuated vir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Noone D,Howell A,Collery R,Devine KM

    更新日期:2001-01-01 00:00:00

  • Potassium transport system of Rhodopseudomonas capsulata.

    abstract::Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Jasper P

    更新日期:1978-03-01 00:00:00

  • Inhibition of citric acid accumulation by manganese ions in Aspergillus niger mutants with reduced citrate control of phosphofructokinase.

    abstract::Mutant strains of Aspergillus niger with reduced citrate control of carbohydrate catabolism (cic mutants) grow faster than the parent strain on media containing 5% (wt/vol) citrate. The mutants tolerated a higher intracellular citrate concentration than the parent strain. One mutant (cic-7/3) contained phosphofructoki...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schreferl G,Kubicek CP,Röhr M

    更新日期:1986-03-01 00:00:00

  • Phosphorylation of lipopolysaccharides in the Antarctic psychrotroph Pseudomonas syringae: a possible role in temperature adaptation.

    abstract::Phosphorylation of lipopolysaccharide (LPS) from a psychrotrophic bacterium, Pseudomonas syringae, from Antarctica was studied by using sucrose gradient-separated membrane fractions. The bacterium was found to possess an LPS kinase which could phosphorylate more LPS postsynthetically at higher temperatures. The phosph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ray MK,Kumar GS,Shivaji S

    更新日期:1994-07-01 00:00:00

  • Cell shape and division in Escherichia coli: experiments with shape and division mutants.

    abstract::Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsI, ftsQ, or ftsZ) required for septation were constructed. Such mutants were able to grow for about two mass doublings at a normal rate at the restri...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Begg KJ,Donachie WD

    更新日期:1985-08-01 00:00:00

  • Possible role of lectins in mycoparasitism.

    abstract::Lectin activity in a host-mycoparasite relationship was demonstrated with Rhizoctonia solani and Trichoderma harzianum. Attachment of O but not A and B erythrocytes to hyphae occurred on R. solani but not on its mycoparasite. This phenomenon, which was Ca2+ and Mn2+ dependent, was prevented by galactose, present in T....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Elad Y,Barak R,Chet I

    更新日期:1983-06-01 00:00:00

  • Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum.

    abstract::The DNA-dependent RNA polymerase (EC from Clostridium acetobutylicum DSM 1731 has been purified to homogeneity and characterized. The purified enzyme was composed of four subunits and had a molecular mass of 370,000 Da. Western immunoblot analysis with polyclonal antibodies against the sigma 70 subunit of Esc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pich A,Bahl H

    更新日期:1991-03-01 00:00:00

  • Promoter of the pertussis toxin operon and production of pertussis toxin.

    abstract::Pertussis toxin (PT), the major virulence factor of Bordetella pertussis, is composed of five different subunits whose genes are organized as an operon. We report the mapping of the promoter region of the PT operon and show that this promoter is only weakly active in Escherichia coli. Bordetella parapertussis and Bord...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nicosia A,Rappuoli R

    更新日期:1987-06-01 00:00:00


    abstract::Freeman, Bob A. (University of Chicago, Chicago, Ill.) and Richard Circo. Effect of tetracyclines on the intracellular amino acids of molds. J. Bacteriol. 86:38-44. 1963.-The tetracycline antibiotics were shown to alter the amino acid metabolism of molds whose growth is not markedly affected. Eight molds were grown in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: FREEMAN BA,CIRCO R

    更新日期:1963-07-01 00:00:00

  • Macromolecular structure and morphology of native glycogen particles isolated from Candida albicans.

    abstract::A polysaccharide-rich particulate fraction was isolated from cytoplasmic extracts of Candida albicans by a procedure using differential centrifugation. The polysaccharide particles obtained after purification with deoxycholate treatment were essentially free of nitrogen and were identified chemically as polyglucosan, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yamaguchi H,Kanda Y,Iwata K

    更新日期:1974-10-01 00:00:00

  • OmpR and LeuO positively regulate the Salmonella enterica serovar Typhi ompS2 porin gene.

    abstract::The Salmonella enterica serovar Typhi ompS2 gene codes for a 362-amino-acid outer membrane protein that contains motifs common to the porin superfamily. It is expressed at very low levels compared to the major OmpC and OmpF porins, as observed for S. enterica serovar Typhi OmpS1, Escherichia coli OmpN, and Klebsiella ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Fernández-Mora M,Puente JL,Calva E

    更新日期:2004-05-01 00:00:00

  • InvB is a type III secretion-associated chaperone for the Salmonella enterica effector protein SopE.

    abstract::SopE is a bacteriophage-encoded effector protein of Salmonella enterica serovar Typhimurium that is translocated into the cytosol of eukaryotic cells by a type III secretion system (TTSS) (W.-D. Hardt, H. Urlaub, and J. E. Galán, Proc. Natl. Acad. Sci. USA 95:2574-2579, 1998; M. W. Wood, R. Rosqvist, P. B. Mullan, M. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee SH,Galán JE

    更新日期:2003-12-01 00:00:00

  • Cellular autolytic activity in synchronized populations of Streptococcus faecium.

    abstract::The autolytic capacity of Streptococcus faecium (S. faecalis ATCC 9790) varied during synchronous cell division. This phenomenon was initially observed in rapidly dividing populations (TD=30 to 33 min) synchronized by a combination of induction and size selection techniques. To minimize the problems inherent in studie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hinks RP,Daneo-Moore L,Shockman GD

    更新日期:1978-02-01 00:00:00

  • Depletion of Glucose Activates Catabolite Repression during Pneumonic Plague.

    abstract::Bacterial pathogenesis depends on changes in metabolic and virulence gene expression in response to changes within a pathogen's environment. The plague-causing pathogen, Yersinia pestis, requires expression of the gene encoding the Pla protease for progression of pneumonic plague. The catabolite repressor protein Crp,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ritzert JT,Lathem WW

    更新日期:2018-05-09 00:00:00

  • SwmB, a 1.12-megadalton protein that is required for nonflagellar swimming motility in Synechococcus.

    abstract::SwmB is required for swimming motility in Synechococcus sp. strain WH8102. This highly repetitive 1.12-MDa polypeptide is associated with the cell surface, where it is arranged in a punctate manner. Inactivation of swmB does not affect the localization of SwmA, an S-layer protein also required for swimming. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McCarren J,Brahamsha B

    更新日期:2007-02-01 00:00:00

  • Release of proteinase from mycelium of Mucor hiemalis.

    abstract::When Mucor hiemalis NRRL 3103 was grown in soybean medium, only a small fraction of the proteinase produced by the organism appeared in the culture filtrate, whereas the bulk of the enzyme was bound to the mycelial surface. Optimal pH of the proteinase ranged from 3.0 to 3.5. Inclusion of sodium chloride or other ioni...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang HL

    更新日期:1967-06-01 00:00:00

  • Organization of rRNA genes in Mycobacterium bovis BCG.

    abstract::The number of rRNA genes in Mycobacterium bovis BCG was examined by Southern hybridization of end-labeled 5S, 16S, and 23S rRNAs with BamHI, PstI, and SalI digests of M. bovis BCG DNA. Each RNA probe gave only one radioactive band with three kinds of DNA digest. These results suggest that M. bovis BCG chromosomes may ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Suzuki Y,Yoshinaga K,Ono Y,Nagata A,Yamada T

    更新日期:1987-02-01 00:00:00

  • Purification, properties, and sequence of glycerol trinitrate reductase from Agrobacterium radiobacter.

    abstract::Glycerol trinitrate (GTN) reductase, which enables Agrobacterium radiobacter to utilize GTN and related explosives as sources of nitrogen for growth, was purified and characterized, and its gene was cloned and sequenced. The enzyme was a 39-kDa monomeric protein which catalyzed the NADH-dependent reductive scission of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Snape JR,Walkley NA,Morby AP,Nicklin S,White GF

    更新日期:1997-12-01 00:00:00

  • Role of SufI (FtsP) in cell division of Escherichia coli: evidence for its involvement in stabilizing the assembly of the divisome.

    abstract::The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Samaluru H,SaiSree L,Reddy M

    更新日期:2007-11-01 00:00:00

  • The Moraxella catarrhalis immunoglobulin D-binding protein MID has conserved sequences and is regulated by a mechanism corresponding to phase variation.

    abstract::The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Möllenkvist A,Nordström T,Halldén C,Christensen JJ,Forsgren A,Riesbeck K

    更新日期:2003-04-01 00:00:00

  • Influence of genes encoding proton-translocating enzymes on suppression of Salmonella typhimurium growth and colonization.

    abstract::Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

    更新日期:1997-11-01 00:00:00

  • Polar targeting of DivIVA in Bacillus subtilis is not directly dependent on FtsZ or PBP 2B.

    abstract::DivIVA is involved in Bacillus subtilis cell division and is located at the cell poles. Previous experiments suggested that the cell division proteins FtsZ and PBP 2B are required for polar targeting of DivIVA. By using outgrowing spores, we show that DivIVA accumulates at the cell poles independent of the presence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hamoen LW,Errington J

    更新日期:2003-01-01 00:00:00

  • Role of the C terminus of FtsK in Escherichia coli chromosome segregation.

    abstract::FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yu XC,Weihe EK,Margolin W

    更新日期:1998-12-01 00:00:00

  • 3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product.

    abstract::The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

    更新日期:1997-06-01 00:00:00

  • Glucose-triggered germination of Bacillus megaterium spores.

    abstract::Triggering of germination in Bacillus megaterium QM B1551 spores with D-glucose was studied. First, the interaction of glucose with spores for less than 1 min resulted in triggering almost 90% of the spores after the glucose was removed by dilution. Therefore only a brief time is needed for glucose to trigger germinat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Racine FM,Dills SS,Vary JC

    更新日期:1979-05-01 00:00:00

  • Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I.

    abstract::Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Shizuya H,Dykhuizen D

    更新日期:1972-11-01 00:00:00

  • Importance of cis determinants and nitrogenase activity in regulated stability of the Klebsiella pneumoniae nitrogenase structural gene mRNA.

    abstract::The Klebsiella pneumoniae nitrogen fixation (nif) mRNAs are unusually stable, with half-lives of 20 to 30 min under conditions favorable to nitrogen fixation (limiting nitrogen, anaerobiosis, temperatures of 30 degrees C). Addition of O2 or fixed nitrogen or temperature increases to 37 degrees C or more result in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Simon HM,Gosink MM,Roberts GP

    更新日期:1999-06-01 00:00:00

  • Two novel membrane proteins, TcpD and TcpE, are essential for conjugative transfer of pCW3 in Clostridium perfringens.

    abstract::The anaerobic pathogen Clostridium perfringens encodes either toxin genes or antibiotic resistance determinants on a unique family of conjugative plasmids that have a novel conjugation region, the tcp locus. Studies of the paradigm conjugative plasmid from C. perfringens, the 47-kb tetracycline resistance plasmid pCW3...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wisniewski JA,Teng WL,Bannam TL,Rood JI

    更新日期:2015-02-15 00:00:00

  • The Vibrio harveyi GTPase CgtAV is essential and is associated with the 50S ribosomal subunit.

    abstract::It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtAV is not essential. Here we show that cgtAV was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtAV did not result in cell elongation. CgtAV is associated with the large ribosomal particle....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sikora AE,Zielke R,Datta K,Maddock JR

    更新日期:2006-02-01 00:00:00