Enteropathogenic Escherichia coli: identification of a gene cluster coding for bundle-forming pilus morphogenesis.

Abstract:

:Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at least 12 open reading frames, including bfpA, that could direct the synthesis of a morphologically normal BFP filament. Within the bfp gene cluster, we identified open reading frames that share homology with other type IV pilus accessory genes and with genes required for transformation competence and protein secretion. Immediately upstream of the bfp gene cluster, we identified a potential replication origin including genes that are predicted to encode proteins homologous with replicase and resolvase. Restriction fragment length polymorphism analysis of DNA from six additional EPEC serotypes showed that the organization of the bfp gene cluster and its juxtaposition with a potential plasmid origin of replication are highly conserved features of the EPEC biotype.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK

doi

10.1128/jb.178.9.2613-2628.1996

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

2613-28

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

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