The LysR-type transcriptional regulator QseD alters type three secretion in enterohemorrhagic Escherichia coli and motility in K-12 Escherichia coli.

abstract：:Ruminococcin A (RumA) is a trypsin-dependent lantibiotic produced by Ruminococcus gnavus E1, a gram-positive strict anaerobic strain isolated from a human intestinal microbiota. A 12.8-kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumA, has been cloned and sequenced. It consisted ...

journal_title：Journal of bacteriology

authors： Gomez A,Ladiré M,Marcille F,Fons M

更新日期：2002-01-01 00:00:00

abstract：:Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

journal_title：Journal of bacteriology

authors： Rocco JM,Churchward G

更新日期：2006-03-01 00:00:00

abstract：:Banerjee, Arun B. (University of Calcutta, Calcutta, India), and S. K. Bose. Biosynthesis of mycobacillin, a new antifungal peptide. II. Relation between streptomycin dependence and biosynthesis. J. Bacteriol. 87:1402-1405. 1964.-A streptomycin-dependent variant was isolated by a single-step mutation process from a st...

journal_title：Journal of bacteriology

authors： BANERJEE AB,BOSE SK

更新日期：1964-06-01 00:00:00

abstract：:Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,00...

journal_title：Journal of bacteriology

authors： Schulman M,Valentino D

更新日期：1976-10-01 00:00:00

abstract：:We described previously a class of mutations (phenotypic designation R2(-)) in Staphylococcus aureus that were unlinked to penicillinase genes but nevertheless imposed constitutive synthesis of this enzyme upon wild-type plasmids. The plasmids affected by these mutations have been found to possess the same maintenance...

journal_title：Journal of bacteriology

authors： Cohen S,Vernon EG,Sweeney HM

更新日期：1970-09-01 00:00:00

abstract：:The bacteriophage lambda cIII gene product regulates the lysogenic pathway. The cIII gene is located in the leftward operon, which is transcribed from the pL promoter. We have previously shown (S. Altuvia and A. B. Oppenheim, J. Bacteriol. 167:415-419, 1986) that mutations that show elevated expression lie within the ...

journal_title：Journal of bacteriology

authors： Kornitzer D,Teff D,Altuvia S,Oppenheim AB

更新日期：1989-05-01 00:00:00

abstract：:A 200-megadalton plasmid was mobilized from Rhizobium japonicum USDA 191 to other Rhizobium strains either that cannot nodulate soybeans or that form Fix- nodules on certain cultivars. The symbiotic properties of the transconjugants indicate that both soybean specificity for nodulation and cultivar specificity for nit...

journal_title：Journal of bacteriology

authors： Appelbaum ER,McLoughlin TJ,O'Connell M,Chartrain N

更新日期：1985-07-01 00:00:00

abstract：:Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends on the AHL synthase SinI and two transcriptional regulators, SinR and ExpR. Our study demonstrates that ectopic expression of the gene rne, coding ...

journal_title：Journal of bacteriology

authors： Baumgardt K,Charoenpanich P,McIntosh M,Schikora A,Stein E,Thalmann S,Kogel KH,Klug G,Becker A,Evguenieva-Hackenberg E

更新日期：2014-04-01 00:00:00

abstract：:narK mutants of Escherichia coli produce wild-type levels of nitrate reductase but, unlike the wild-type strain, do not accumulate nitrite when grown anaerobically on a glucose-nitrate medium. Comparison of the rates of nitrate and nitrite metabolism in cultures growing anaerobically on glucose-nitrate medium revealed...

journal_title：Journal of bacteriology

authors： DeMoss JA,Hsu PY

更新日期：1991-06-01 00:00:00

abstract：:Bacterial uptake of phosphate is usually accomplished via high-affinity transporters that are commonly regulated by two-component systems, which are activated when the concentration of phosphate is low. Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transpo...

journal_title：Journal of bacteriology

authors： Gebhard S,Busby JN,Fritz G,Moreland NJ,Cook GM,Lott JS,Baker EN,Money VA

更新日期：2014-10-01 00:00:00

abstract：:Saccharomyces cerevisiae cells, grown on a fermentable carbon source, display two growth phases before they enter the stationary phase: a rapid phase (log phase) followed by a slow phase. It was previously shown that a subunit of the yeast RNA polymerase II, RPB4, positively affects the activity of the enzyme in post-...

journal_title：Journal of bacteriology

authors： Choder M

更新日期：1993-10-01 00:00:00

abstract：:Bacterial lipases play important roles in bacterial metabolism and environmental response. Our laboratory recently discovered that a novel lipoprotein lysophospholipase, VolA, localizes on the surface of the Gram-negative aquatic pathogen Vibrio cholerae. VolA functions to cleave exogenous lysophosphatidylcholine, fre...

journal_title：Journal of bacteriology

authors： Pride AC,Guan Z,Trent MS

更新日期：2014-04-01 00:00:00

abstract：:The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...

journal_title：Journal of bacteriology

authors： Uchiyama T,Kaneko R,Yamaguchi J,Inoue A,Yanagida T,Nikaidou N,Regue M,Watanabe T

更新日期：2003-03-01 00:00:00

abstract：:The gram-positive bacterium Bacillus subtilis contains two minimal Tat translocases, TatAdCd and TatAyCy, which are each involved in the secretion of one or more specific protein substrates. We have investigated the subcellular localization of the TatA components by employing C-terminal green fluorescent protein (GFP)...

journal_title：Journal of bacteriology

authors： Ridder AN,de Jong EJ,Jongbloed JD,Kuipers OP

更新日期：2009-07-01 00:00:00

abstract：:The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...

journal_title：Journal of bacteriology

authors： Briles EB,Tomasz A

更新日期：1975-04-01 00:00:00

abstract：:Mycolic acids are a key component of the mycobacterial cell wall, providing structure and forming a major permeability barrier. In Mycobacterium tuberculosis mycolic acids are synthesized by type I and type II fatty acid synthases. One of the enzymes of the type II system is encoded by fabG1. We demonstrate here that ...

journal_title：Journal of bacteriology

authors： Parish T,Roberts G,Laval F,Schaeffer M,Daffé M,Duncan K

更新日期：2007-05-01 00:00:00

abstract：:A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....

journal_title：Journal of bacteriology

authors： Sonstein SA,Hammel JM,Bondi A

更新日期：1971-08-01 00:00:00

abstract：:The Escherichia coli mutant Y16, which shows thermosensitive colony formation and filamentation with reduced amounts of penicillin-binding protein 3 (PBP3), has mutations in the ftsI gene encoding PBP3 and in the ftsH gene. The ftsI mutation markedly reduces the amount of PBP3 at 42 degrees C, whereas the amount of th...

journal_title：Journal of bacteriology

authors： Begg KJ,Tomoyasu T,Donachie WD,Khattar M,Niki H,Yamanaka K,Hiraga S,Ogura T

更新日期：1992-04-01 00:00:00

abstract：:The allosteric pattern of control for 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase has previously been shown to be strongly conserved among the member species of a given genus in bacteria. The implications of this finding as a procedural tool of bacterial phylogeny were pursued by a study of two organ...

journal_title：Journal of bacteriology

authors： Jensen RA,Stenmark SL

更新日期：1970-03-01 00:00:00

abstract：:Nine high copy number mutations of plasmid R6-5, representing five phenotypically distinct groups, have been identified by DNA sequencing. In each mutant plasmid examined, a single nucleotide change was found. The effects of the mutations on possible gene products, and DNA-RNA secondary structure, were analyzed and co...

journal_title：Journal of bacteriology

authors： Brady G,Frey J,Danbara H,Timmis KN

更新日期：1983-04-01 00:00:00

abstract：:We have constructed a suicide vector, pU1800, containing the transposable element TnphoA (Tn5 IS50L::phoA), for the purpose of producing protein fusions in vivo between the Escherichia coli alkaline phosphatase (APase) and proteins of the facultative photoheterotroph, Rhodobacter sphaeroides. We introduced TnphoA into...

journal_title：Journal of bacteriology

authors： Moore MD,Kaplan S

更新日期：1989-08-01 00:00:00

abstract：:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...

journal_title：Journal of bacteriology

authors： Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

更新日期：1995-01-01 00:00:00

abstract：:Capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (W. Godchaux III and E. R. Leadbetter, J. Bacteriol. 144:592-602, 1980). One of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, N-acylated versions of capnine. The lipids were foun...

journal_title：Journal of bacteriology

authors： Godchaux W 3rd,Leadbetter ER

更新日期：1983-03-01 00:00:00

abstract：:Eight metH mutants in Salmonella typhimurium with closely linked sites of mutation which could grow only on methionine were isolated from a metE mutant deficient in N(5)-methyltetrahydropteroyltriglutamate-homocysteine transmethylase; their deficiency in cobalamin-dependent N(5)-methyltetrahydrofolate-homocysteine tra...

journal_title：Journal of bacteriology

authors： Childs JD,Smith DA

更新日期：1969-10-01 00:00:00

abstract：:The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various concentrations of oxygen or types of growth substrates. Spectrophotometric analysis revealed that the quantity and type...

journal_title：Journal of bacteriology

authors： Ensley BD Jr,Finnerty WR

更新日期：1980-06-01 00:00:00

abstract：:The single polar flagellum of Pseudomonas aeruginosa plays an important role in the pathogenesis of infection by this organism. However, regulation of the assembly of this organelle has not been delineated. In analyzing the sequence available at the Pseudomonas genome database, an open reading frame (ORF), flanked by ...

journal_title：Journal of bacteriology

authors： Dasgupta N,Arora SK,Ramphal R

更新日期：2000-01-01 00:00:00

abstract：:The lytD1 mutant of Escherichia coli exhibits temperature-sensitive growth which is attributed to cellular autolysis at the restrictive temperature. Either of two cloned phage lambda genes, identified as cI and cro, suppressed the lytD1(Ts) lysis phenotype, suggesting that lytD encodes a DNA-binding protein with a DNA...

journal_title：Journal of bacteriology

authors： Dai DX,Ishiguro EE

更新日期：1991-01-01 00:00:00

abstract：:The plasmid-encoded epidermin biosynthetic gene epiC of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using the T7 RNA polymerase-promoter system, and the gene product EpiC was identified by Western blotting (immunoblotting) with an anti-EpiC-peptide antiserum. EpiC was a hydrophobic but solub...

journal_title：Journal of bacteriology

authors： Kupke T,Gotz F

更新日期：1996-03-01 00:00:00

abstract：:Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...

journal_title：Journal of bacteriology

authors： Brambl R

更新日期：1975-06-01 00:00:00

abstract：:The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobact...

journal_title：Journal of bacteriology

authors： Steffen DL,Cocks GT,Wilson AC

更新日期：1972-06-01 00:00:00