Abstract：
:Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The optimal partial pressure of O(2) was increased, and the partial pressure of O(2) range for C(2)H(2) reduction was extended by adding H(2). A supply of succinate to bacteroids resulted in effects similar to those obtained by adding H(2). Both H(2) and succinate provided respiratory protection for the N(2)-fixing system in bacteroids. The oxidation of H(2) by bacteroids increased the steady-state pool of ATP by 20 to 40%. In the presence of 50 mM iodoacetate, which caused much greater inhibition of endogenous respiration than of H(2) oxidation, the addition of H(2) increased the steady-state pool of ATP in bacteroids by 500%. Inhibitor evidence and an absolute requirement for O(2) indicated that the H(2)-stimulated ATP synthesis occurred through oxidative phosphorylation. In the presence of 50 mM iodoacetate, H(2)-dependent ATP synthesis occurred at a rate sufficient to support nitrogenase activity. The addition of H(2) to H(2) uptake-negative strains of R. japonicum had no effect on ATP formation or C(2)H(2) reduction. It is concluded that the H(2)-oxidizing system in H(2) uptake-positive bacteroids benefits the N(2)-fixing process by providing respiratory protection of the O(2)-labile nitrogenase proteins and generating ATP to support maximal rates of C(2)H(2) reduction by oxidation of the H(2) produced from the nitrogenase system.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Emerich DW,Ruiz-Argüeso T,Ching TM,Evans HJdoi
10.1128/JB.137.1.153-160.1979subject
Has Abstractpub_date
1979-01-01 00:00:00pages
153-60issue
1eissn
0021-9193issn
1098-5530journal_volume
137pub_type
杂志文章abstract：:Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose w...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.119.2.484-493.1974
更新日期：1974-08-01 00:00:00
abstract：:Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.) and Joseph Greenberg. Resistance and cross resistance of Escherichia coli S mutants to the radiomimetic agent nitrofurazone. J. Bacteriol. 85:1208-1216. 1963.-Cross-resistance relationships are described for 73 mutants of Escherichia coli s...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.85.6.1208-1216.1963
更新日期：1963-06-01 00:00:00
abstract：:Saccharomyces cerevisiae cells, grown on a fermentable carbon source, display two growth phases before they enter the stationary phase: a rapid phase (log phase) followed by a slow phase. It was previously shown that a subunit of the yeast RNA polymerase II, RPB4, positively affects the activity of the enzyme in post-...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.175.19.6358-6363.1993
更新日期：1993-10-01 00:00:00
abstract：:Strain 4R is a phycocyanin-minus mutant of the unicellular cyanobacterium Synechocystis sp. strain 6803. Although it lacks the light-harvesting protein phycocyanin, 4R has normal levels of phycocyanin (cpc) transcripts. Sequence analysis of the cpcB gene encoding the phycocyanin beta subunit shows an insertion mutatio...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.177.23.6798-6803.1995
更新日期：1995-12-01 00:00:00
abstract：:Escherichia coli fhuF mutants, a sufS::MudI mutant, and a sufD::MudI mutant were found to have the same phenotype: the inability to use ferrioxamine B as an iron source in a plate assay. In addition, the sufS and sufD genes were shown to be regulated by the iron-dependent Fur repressor. Sequence analysis revealed that...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.181.10.3307-3309.1999
更新日期：1999-05-01 00:00:00
abstract：:Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.140.2.479-489.1979
更新日期：1979-11-01 00:00:00
abstract：:Soluble c-type cytochromes are central to metabolism of C1 compounds in methylotrophic bacteria. In order to characterize the role of c-type cytochromes in methane-utilizing bacteria (methanotrophs), we have purified four different cytochromes, cytochromes c-554, c-553, c-552, and c-551, from the marine methanotroph M...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.172.9.5360-5367.1990
更新日期：1990-09-01 00:00:00
abstract：:d-Arabinose isomerase (EC 5.3.1.3) has been isolated from l-fucose-induced cultures of Escherichia coli K-12 and d-arabinose-induced cultures of E. coli B/r. Both enzymes were homogeneous in an ultracentrifuge and migrated as single bands upon disc electrophoresis in acrylamide gels. The s(20,w) was 14.5 x 10(-13) sec...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.113.2.687-696.1973
更新日期：1973-02-01 00:00:00
abstract：:Replication initiation depends on origin recognition, helicase, and primase activities. In phage P4, a second DNA region, the cis replication region (crr), is also required for replication initiation. The multifunctional alpha protein of phage P4, which is essential for DNA replication, combines the three aforemention...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.179.13.4087-4095.1997
更新日期：1997-07-01 00:00:00
abstract：:In previous work with membranes of Bacillus subtilis, the succinate dehydrogenase complex was isolated by immunoprecipitation of Triton X-100-solubilized membranes. The complex included a polypeptide with an apparent molecular weight of 19,000, probably attributable to apocytochrome. This paper reports the further cha...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.144.3.933-940.1980
更新日期：1980-12-01 00:00:00
abstract：:The effect of dihydrostreptomycin on the incorporation of amino acids into protein in antibiotic-deprived cells of a streptomycin-dependent strain of Escherichia coli B has been compared with its effect on protein synthesis in extracts from cells of the same strain. Stimulation of phenylalanine incorporation into prot...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.100.1.247-253.1969
更新日期：1969-10-01 00:00:00
abstract：:The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium do...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.172.12.7275-7277.1990
更新日期：1990-12-01 00:00:00
abstract：:Plasmids for high-level expression of penicillin-binding protein 6 (PBP6) were constructed, giving rise to overproduction of PBP6 under the control of the lambda pR promoter in either the periplasmic or the cytoplasmic space. In contrast to penicillin-binding protein 5 (PBP5), the presence of high amounts of PBP6 in t...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.174.23.7572-7578.1992
更新日期：1992-12-01 00:00:00
abstract：:The type III secretion system (T3SS) encoded by Salmonella pathogenicity island 2 (SPI-2) is involved in systemic infection and intracellular replication of Salmonella enterica serovar Typhimurium. In this study, we investigated the function of SsaE, a small cytoplasmic protein encoded within the SPI-2 locus, which sh...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00863-09
更新日期：2009-11-01 00:00:00
abstract：:The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF- mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the respon...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.188.2.801-804.2006
更新日期：2006-01-01 00:00:00
abstract：:A superfamily of transport proteins, which includes the lactose permease of Escherichia coli, contains a highly conserved motif, G-X-X-X-D/E-R/K-X-G-R/K-R/K, in the loops that connect transmembrane segments 2 and 3 and transmembrane segments 8 and 9. Previous analysis of this motif in the lactose permease (A. E. Jesse...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.179.8.2616-2622.1997
更新日期：1997-04-01 00:00:00
abstract：:Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could mi...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00728-06
更新日期：2006-12-01 00:00:00
abstract：:The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.183.11.3328-3335.2001
更新日期：2001-06-01 00:00:00
abstract：:From several presumably pure cultures of Thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. One was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not s...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.143.1.448-454.1980
更新日期：1980-07-01 00:00:00
abstract：:Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.104.2.933-939.1970
更新日期：1970-11-01 00:00:00
abstract：:The ASM Conference on Mechanisms of Interbacterial Cooperation and Competition was held in Washington DC, from 1 to 4 March 2017. The conference provided an international forum for sociomicrobiologists from different disciplines to present and discuss new findings. The meeting covered a wide range of topics, spanning ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00403-17
更新日期：2017-08-14 00:00:00
abstract：:ZoBell, Claude E. (University of California, La Jolla) and Andre B. Cobet. Growth, reproduction, and death rates of Escherichia coli at increased hydrostatic pressures. J. Bacteriol. 84:1228-1236. 1962.-Pressures ranging from 100 to 500 atm were found to retard the growth and reproduction of Escherichia coli in nutrie...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.84.6.1228-1236.1962
更新日期：1962-12-01 00:00:00
abstract：:A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.128.2.668-670.1976
更新日期：1976-11-01 00:00:00
abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.173.1.86-93.1991
更新日期：1991-01-01 00:00:00
abstract：:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mu...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.178.2.410-417.1996
更新日期：1996-01-01 00:00:00
abstract：:A number of laboratories are now engaged in the genetic analysis of Candida albicans. This diploid yeast, the major fungal pathogen of humans, is imperfect. Parasexual techniques have been devised for complementation and recombination analysis in this organism. This paper attempts to address the question of the extent...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.156.2.498-506.1983
更新日期：1983-11-01 00:00:00
abstract：:Lrp (leucine-responsive regulatory protein) is a global regulator of metabolism in Escherichia coli (J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). The lrp genes from three other enteric microorganisms, Enterobacter aerogenes, Klebsiella aerogenes, and Salmonella typhimurium, were cloned and sequen...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.177.6.1624-1626.1995
更新日期：1995-03-01 00:00:00
abstract：:Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.02474-14
更新日期：2015-04-01 00:00:00
abstract：:Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.155.1.159-168.1983
更新日期：1983-07-01 00:00:00
abstract：:Burkholderia cenocepacia strain K56-2, a representative of the Burkholderia cepacia complex, is part of the epidemic and clinically problematic ET12 lineage. The strain produced plant tissue watersoaking (ptw) on onion tissue, which is a plant disease-associated trait. Using plasposon mutagenesis, mutants in the ptw p...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.186.18.6015-6024.2004
更新日期：2004-09-01 00:00:00
