Protoplast formation in Escherichia coli.

abstract：:The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...

journal_title：Journal of bacteriology

authors： Koudelka AP,Hufnagel LA,Koudelka GB

更新日期：2004-11-01 00:00:00

abstract：:A genetic approach was used to establish the route of UMP biosynthesis in Sulfolobus acidocaldarius, a member of the hyperthermophilic division (the Crenarchaeota) of the Archaea domain. Pyrimidine auxotrophs of S. acidocaldarius DG6 were isolated by direct selection and by brute-force methods. Enzymatic assay of extr...

journal_title：Journal of bacteriology

authors： Grogan DW,Gunsalus RP

更新日期：1993-03-01 00:00:00

abstract：:Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

journal_title：Journal of bacteriology

authors： Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

更新日期：1990-09-01 00:00:00

abstract：:The sigA and sigB genes of Mycobacterium tuberculosis encode two sigma 70-like sigma factors of RNA polymerase. While transcription of the sigA gene is growth rate independent, sigB transcription is increased during entry into stationary phase. The sigA gene transcription is unresponsive to environmental stress but th...

journal_title：Journal of bacteriology

authors： Hu Y,Coates AR

更新日期：1999-01-01 00:00:00

abstract：:A combined physical and genetic map of the genome of strain SG24 of Vibrio cholerae O139 Bengal, a novel non-O1 strain having epidemic potential, has been constructed by using the enzymes NotI, SfiI, and CeuI. The genome of SG24 is circular, and the genome size is about 3. 57 Mb. The linkages between 47 NotI and 32 Sf...

journal_title：Journal of bacteriology

authors： Khetawat G,Bhadra RK,Kar S,Das J

更新日期：1998-09-01 00:00:00

abstract：:Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...

journal_title：Journal of bacteriology

authors： JOHNSON RC,ROGERS P

更新日期：1964-12-01 00:00:00

abstract：:In wild-type Escherichia coli, fumarate reductase encoded by the frd operon is inducible by its substrate in the absence of molecular oxygen and nitrate. Synthesis of this enzyme under permissive conditions requires the fnr+ gene product, which is believed to be a pleiotropic regulatory protein that activates transcri...

journal_title：Journal of bacteriology

authors： Iuchi S,Kuritzkes DR,Lin EC

更新日期：1985-03-01 00:00:00

abstract：:We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus c...

journal_title：Journal of bacteriology

authors： Huang Y,Anderson R

更新日期：1991-01-01 00:00:00

abstract：:The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetracycline resistance we...

journal_title：Journal of bacteriology

authors： McNicholas P,Chopra I,Rothstein DM

更新日期：1992-12-01 00:00:00

abstract：:Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid inco...

journal_title：Journal of bacteriology

authors： Hashimoto-Gotoh T,Inselburg J

更新日期：1979-08-01 00:00:00

abstract：:Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that they encode. Here, we...

journal_title：Journal of bacteriology

authors： Garriss G,Poulin-Laprade D,Burrus V

更新日期：2013-05-01 00:00:00

abstract：:Escherichia coli strains mutant in the starvation gene cstC grow normally in a mineral salts medium but are impaired in utilizing amino acids as nitrogen sources. They are also compromised in starvation survival, where amino acid catabolism is important. The cstC gene encodes a 406-amino-acid protein that closely rese...

journal_title：Journal of bacteriology

authors： Fraley CD,Kim JH,McCann MP,Matin A

更新日期：1998-08-01 00:00:00

abstract：:Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...

journal_title：Journal of bacteriology

authors： Alphen WV,Lugtenberg B

更新日期：1977-08-01 00:00:00

abstract：:Nisin is a cationic antimicrobial peptide that belongs to the group of lantibiotics. It is thought to form oligomeric pores in the target membrane by a mechanism that requires the transmembrane electrical potential delta psi and that involves local pertubation of the lipid bilayer structure. Here we show that nisin do...

journal_title：Journal of bacteriology

authors： Moll GN,Clark J,Chan WC,Bycroft BW,Roberts GC,Konings WN,Driessen AJ

更新日期：1997-01-01 00:00:00

abstract：:Strain IMCC9480 is a novel member of the family Oxalobacteraceae of the Betaproteobacteria, isolated from the Arctic Ocean by dilution-to-extinction culturing. Here we present the draft genome sequence of strain IMCC9480. The genome is predicted to contain genes for xanthorhodopsin, retinoid biosynthesis, carbon monox...

journal_title：Journal of bacteriology

authors： Oh HM,Lee K,Jang Y,Kang I,Kim HJ,Kang TW,Kim SY,Cho JC

更新日期：2011-07-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 can fix N(2) under oxic conditions, and the activity of nitrogen fixation occurs exclusively in heterocysts, cells differentiated from vegetative cells in response to a limitation of a combined-nitrogen source in the growth medium. At the late stages of heter...

journal_title：Journal of bacteriology

authors： Shi L,Li JH,Cheng Y,Wang L,Chen WL,Zhang CC

更新日期：2007-07-01 00:00:00

abstract：:Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...

journal_title：Journal of bacteriology

authors： Roggiani M,Hahn J,Dubnau D

更新日期：1990-07-01 00:00:00

abstract：:The control of the synthesis of certain key enzymes of aromatic amino acid biosynthesis was studied. Tyrosine represses the first enzyme of the 3-deoxy-d-arabino heptulosonic acid 7-phosphate pathway, DAHP synthetase, as well as shikimate kinase and chorismate mutase about fivefold in cultures grown under conditions l...

journal_title：Journal of bacteriology

authors： Nester EW,Jensen RA,Nasser DS

更新日期：1969-01-01 00:00:00

abstract：:The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobact...

journal_title：Journal of bacteriology

authors： Steffen DL,Cocks GT,Wilson AC

更新日期：1972-06-01 00:00:00

abstract：:A strain of Serratia marcescens was found to produce a bacteriocin that inhibits the growth of certain Escherichia coli strains. This inhibition was bacteriocidal rather than bacteriostatic and was not caused by a bacteriophage. Whereas the bacteriocin was inactive on the 7 Serratia strains tested, it killed 11 of the...

journal_title：Journal of bacteriology

authors： Foulds JD,Shemin D

更新日期：1969-09-01 00:00:00

abstract：:Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...

journal_title：Journal of bacteriology

authors： Hutson JY,Downing M

更新日期：1968-10-01 00:00:00

abstract：:A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology u...

journal_title：Journal of bacteriology

authors： Borgia PT,Gokul NK,Phillips GJ

更新日期：1985-12-01 00:00:00

abstract：:The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR...

journal_title：Journal of bacteriology

authors： Ramon AM,Porta A,Fonzi WA

更新日期：1999-12-01 00:00:00

abstract：:This paper reports the partial characterization of two temperature-sensitive mutants of Escherichia coli with alterations in ribosomal ribonucleic acid (rRNA) metabolism at the restrictive temperature. Both mutants continue to synthesize deoxyribonucleic acid and protein at 42 C but showed little or no accumulation of...

journal_title：Journal of bacteriology

authors： Jackson JM 3rd,Chaney SG

更新日期：1976-03-01 00:00:00

abstract：:Lactobacillus brevis transports glucose and the nonmetabolizable glucose analog 2-deoxyglucose via a proton symport mechanism that is allosterically inhibited by the seryl-phosphorylated derivative of HPr, the small phosphocarrier protein of the phosphotransferase system. We have demonstrate that S46DHPr, a mutant ana...

journal_title：Journal of bacteriology

authors： Ye JJ,Saier MH Jr

更新日期：1995-04-01 00:00:00

abstract：:A delta tyrT::kan mutant from Escherichia coli K-12 (DTK-12) shows a transient growth lag that is caused by glycine starvation (U. Michelsen, M. Bösl, T. Dingermann, and H. Kersten, J. Bacteriol. 171:5987-5994, 1989). The same deletion, transduced into the relA1 spoT1 mutant CA274 to construct strain DTC274, caused co...

journal_title：Journal of bacteriology

authors： Bösl M,Kersten H

更新日期：1994-01-01 00:00:00

abstract：:Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...

journal_title：Journal of bacteriology

authors： Claus H,Friedrich A,Frosch M,Vogel U

更新日期：2000-03-01 00:00:00

abstract：:A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

journal_title：Journal of bacteriology

authors： Von Tersch MA,Carlton BC

更新日期：1984-12-01 00:00:00

abstract：:Cells of Candida albicans WO-1 switch frequently and reversibly between two colony-forming phenotypes, white and opaque. In the white form, budding cells appear similar to those of most other strains of C. albicans, but in the opaque form, budding cells are larger, are bean shaped, and possess pimples on the wall. The...

journal_title：Journal of bacteriology

authors： Anderson J,Mihalik R,Soll DR

更新日期：1990-01-01 00:00:00

abstract：:Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...

journal_title：Journal of bacteriology

authors： Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

更新日期：1972-03-01 00:00:00