The helicase domain of phage P4 alpha protein overlaps the specific DNA binding domain.

Abstract:

:Replication initiation depends on origin recognition, helicase, and primase activities. In phage P4, a second DNA region, the cis replication region (crr), is also required for replication initiation. The multifunctional alpha protein of phage P4, which is essential for DNA replication, combines the three aforementioned activities on a single polypeptide chain. Protein domains responsible for the activities were identified by mutagenesis. We show that mutations of residues G506 and K507 are defective in vivo in phage propagation and in unwinding of a forked helicase substrate. This finding indicates that the proposed P loop is essential for helicase activity. Truncations of gene product alpha (gp alpha) demonstrated that 142 residues of the C terminus are sufficient for specifically binding ori and crr DNA. The minimal binding domain retains gp alpha's ability to induce loop formation between ori and crr. In vitro and in vivo analysis of short C-terminal truncations indicate that the C terminus is needed for helicase activity as well as for specific DNA binding.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ziegelin G,Calendar R,Lurz R,Lanka E

doi

10.1128/jb.179.13.4087-4095.1997

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

4087-95

issue

13

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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