Comparison of lipid composition of pleuropneumonia-like and L-type organisms.

Abstract:

:Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like organisms that required cholesterol for growth and in L-1, the L-form of Streptobacillus moniliformis. Decreasing amounts were found in nonsterol-requiring strains of pleuropneumonia-like organisms, the stable L-form of Proteus, salt-requiring L-forms, and finally, the bacterial parents of the L-type organisms. Nonsterol-requiring pleuropneumonia-like organisms could synthesize their own nonsaponifiable lipid, but sterol-requiring strains could not. Ability or inability to synthesize nonsaponifiable lipid was often carried over to the L-type organisms from the bacterial parent. More nonsaponifiable lipid was found in the sedimentable fraction (cell membrane) than in the nonsedimentable fraction (protoplasm) of both pleuropneumonia-like and L-type organisms. None of the nonsaponifiable lipid synthesized by any organism tested appeared to be 3-beta-hydroxy sterol. Differences in ability of various organisms to take up cholesterol and to be lysed by digitonin were established.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

SMITH PF,ROTHBLAT GH

doi

10.1128/JB.83.3.500-506.1962

keywords:

subject

Has Abstract

pub_date

1962-03-01 00:00:00

pages

500-6

eissn

0021-9193

issn

1098-5530

journal_volume

83

pub_type

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