Transduction by phiBB-1, a bacteriophage of Borrelia burgdorferi.

Abstract:

:We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned fragment of phage DNA, and the resulting construct was transformed into B. burgdorferi CA-11.2A cells. The kan cassette recombined into a resident cp32 and was stably maintained. The cp32 containing the kan cassette was packaged by phiBB-1 released from this B. burgdorferi strain. phiBB-1 has been used to transduce this antibiotic resistance marker into naive CA-11.2A cells, as well as two other strains of B. burgdorferi. This is the first direct evidence of a mechanism for lateral gene transfer in B. burgdorferi.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Eggers CH,Kimmel BJ,Bono JL,Elias AF,Rosa P,Samuels DS

doi

10.1128/JB.183.16.4771-4778.2001

keywords:

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

4771-8

issue

16

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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