In vivo observation of cell division of anaerobic hyperthermophiles by using a high-intensity dark-field microscope.

Abstract:

:To study growth and cell division of anaerobic hyperthermophilic archaea in vivo, a cultivation technique using glass capillaries was developed. At temperatures of 90 to 98 degrees C, at least 10 successive cell divisions of Pyrodictium abyssi TAG 11 were documented. Cells divide by binary fission. Visualized under a modified dark-field microscope, the formation of cannulae, which finally connected all cells, was observed. The cannulae elongated at 1.0 to 1.5 micrometers/min and reached final lengths of between 30 and 150 micrometers. A "snapping division"-like mode of cell fission was discovered for Thermoproteus tenax.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Horn C,Paulmann B,Kerlen G,Junker N,Huber H

doi

10.1128/JB.181.16.5114-5118.1999

keywords:

subject

Has Abstract

pub_date

1999-08-01 00:00:00

pages

5114-8

issue

16

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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