Identification of proteins of the outer (L and P) rings of the flagellar basal body of Escherichia coli.

Abstract:

:Synthesis of the Salmonella typhimurium hook protein from the gene cloned on a multicopy plasmid results in partial suppression of the flagellar assembly defects of certain classes of Escherichia coli mutants (K. Ohnishi, M. Homma, K. Kutsukake, and T. Iino, J. Bacteriol, 169:1485-1488, 1987). This phenomenon allowed hook-basal body complexes from such mutants to be purified and analyzed by electron microscopy and gel electrophoresis. The absence of the P and L rings in such structures was found to correlate with the absence of proteins of apparent molecular weight 39,000 and 26,000, respectively. Gene-polypeptide correlations from other studies enabled us to complete gene-polypeptide-structure correspondences for these two proteins as flaM----39-kilodalton protein----P ring and flaY----26-kilodalton protein----L ring.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Jones CJ,Homma M,Macnab RM

doi

10.1128/jb.169.4.1489-1492.1987

subject

Has Abstract

pub_date

1987-04-01 00:00:00

pages

1489-92

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

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