Toxic properties of the cell wall of gram-positive bacteria.

abstract：:Most mutations in the yajC-secDF operon identified via genetic screens confer a cold-sensitive growth phenotype. Here we report that two of these mutations confer this cold-sensitive phenotype by inactivating the SecDF-YajC complex in protein translocation. ...

journal_title：Journal of bacteriology

authors： Nouwen N,Driessen AJ

更新日期：2005-10-01 00:00:00

abstract：:We previously described 14 rare spontaneous morphological mutants of Candida albicans that were associated with chromosomal aberrations (E. P. Rustchenko-Bulgac, F. Sherman, and J. B. Hicks, J. Bacteriol. 172:1276-1283, 1990). Improved conditions for separation of chromosomes, as well as hybridization probes, were use...

journal_title：Journal of bacteriology

authors： Rustchenko-Bulgac EP

更新日期：1991-10-01 00:00:00

abstract：:The Bacillus subtilis skin element confers resistance to arsenate and arsenite. The ars operon in the skin element contains four genes in the order arsR, ORF2, arsB, and arsC. Three of these genes are homologous to the arsR, arsB, and arsC genes from the staphylococcal plasmid pI258, while no homologs of ORF2 have bee...

journal_title：Journal of bacteriology

authors： Sato T,Kobayashi Y

更新日期：1998-04-01 00:00:00

abstract：:Two replicons were isolated independently from different IncHI1 plasmids. One was isolated from R27, and a second was isolated from pIP522. We demonstrate, by DNA-DNA hybridization experiments, that these maintenance regions are different and that they are specific to, and carried by, all IncHI1 plasmids tested. In vi...

journal_title：Journal of bacteriology

authors： Gabant P,Newnham P,Taylor D,Couturier M

更新日期：1993-12-01 00:00:00

abstract：:The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...

journal_title：Journal of bacteriology

authors： Ehlert K,Tschierske M,Mori C,Schröder W,Berger-Bächi B

更新日期：2000-05-01 00:00:00

abstract：:The impact of arsenite [As(III)] on several levels of cellular metabolism and gene regulation was examined in Pseudomonas aeruginosa. P. aeruginosa isogenic mutants devoid of antioxidant enzymes or defective in various metabolic pathways, DNA repair systems, metal storage proteins, global regulators, or quorum sensing...

journal_title：Journal of bacteriology

authors： Parvatiyar K,Alsabbagh EM,Ochsner UA,Stegemeyer MA,Smulian AG,Hwang SH,Jackson CR,McDermott TR,Hassett DJ

更新日期：2005-07-01 00:00:00

abstract：:Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycin...

journal_title：Journal of bacteriology

authors： Popham DL,Meador-Parton J,Costello CE,Setlow P

更新日期：1999-10-01 00:00:00

abstract：:An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...

journal_title：Journal of bacteriology

authors： White RH

更新日期：1988-10-01 00:00:00

abstract：:Agrobacterium tumefaciens transfers single-stranded DNAs (T strands) into plant cells. VirE1 and VirE2, which is a single-stranded DNA binding protein, are important for tumorigenesis. We show that T strands and VirE2 can enter plant cells independently and that export of VirE2, but not of T strands, depends on VirE1....

journal_title：Journal of bacteriology

authors： Sundberg C,Meek L,Carroll K,Das A,Ream W

更新日期：1996-02-01 00:00:00

abstract：:Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthes...

journal_title：Journal of bacteriology

authors： Kuo TT,Stocker BA

更新日期：1972-10-01 00:00:00

abstract：:A systematic survey for the presence of plasmids in 17 different xenobiotic-degrading Sphingomonas strains was performed. In almost all analyzed strains, two to five plasmids with sizes of about 50 to 500 kb were detected by using pulsed-field gel electrophoresis. A comparison of plasmid preparations untreated or trea...

journal_title：Journal of bacteriology

authors： Basta T,Keck A,Klein J,Stolz A

更新日期：2004-06-01 00:00:00

abstract：:In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...

journal_title：Journal of bacteriology

authors： Li Z,Boyd D,Reindl M,Goldberg MB

更新日期：2014-01-01 00:00:00

abstract：:Bacterial chemotaxis is based on modulation of the probability to switch the direction of flagellar rotation. Responses to many stimuli are transduced by a two-component system via reversible phosphorylation of CheY, a small cytoplasmic protein that directly interacts with the switch complex at the flagellar motor. We...

journal_title：Journal of bacteriology

authors： Montrone M,Oesterhelt D,Marwan W

更新日期：1996-12-01 00:00:00

abstract：:Host range mutants of phage T1 (T1h), which productively infected tonB mutants of Escherichia coli, were isolated. The phage mutants were inactivated by isolated outer membranes of E. coli in contrast to the wild-type phage, which only adsorbed reversibly. For the infection process, the tonB function is apparently onl...

journal_title：Journal of bacteriology

authors： Hantke K,Braun V

更新日期：1978-07-01 00:00:00

abstract：:Aspergillus fumigatus secretes a number of glycosidases into the culture medium when the cells are grown in a mineral salts medium containing guar flour (a galactomannan) as the carbon source. At least some of these glycosidases have been reported to be glycoproteins having N-linked oligosaccharides. In this study, we...

journal_title：Journal of bacteriology

authors： Elbein AD,Mitchell M,Molyneux RJ

更新日期：1984-10-01 00:00:00

abstract：:Mutation of the spoVB gene in Bacillus subtilis causes the production of spores containing a defective cortex and unable to acquire heat resistance. The spoVB locus is highly linked to another spo locus, spoIIIF, characterized by a single mutation (I. L. Lamont and J. Mandelstam, J. Gen. Microbiol. 130:1253-1261, 1984...

journal_title：Journal of bacteriology

authors： Popham DL,Stragier P

更新日期：1991-12-01 00:00:00

abstract：:Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

journal_title：Journal of bacteriology

authors： Hahn J,Dubnau D

更新日期：1985-06-01 00:00:00

abstract：:The gonococcal lsi-6 locus was cloned and shown by DNA sequence analysis to have homology with the E. coli rfaD gene, which encodes ADP-L-glycero-D-mannoheptose epimerase. This enzyme is involved in the biosynthesis of the lipopolysaccharide precursor ADP-L-glycero-D-mannoheptose. A site-directed frameshift mutation i...

journal_title：Journal of bacteriology

authors： Drazek ES,Stein DC,Deal CD

更新日期：1995-05-01 00:00:00

abstract：:Lysates of Escherichia coli Ymel obtained from cultures grown in the absence of tryptophan in minimal medium supplemented with 0.1% casein hydrolysate show an approximate fivefold increase in steady-state specific activity of both anthranilate synthetase and tryptophan synthetase A protein relative to cultures grown i...

journal_title：Journal of bacteriology

authors： Stubbs JD,Stubbs EA

更新日期：1971-12-01 00:00:00

abstract：:Two key elements that are thought to be required for replication initiation in eubacteria are the DnaA protein, a trans-acting factor, and the replication origin, a cis-acting element. As a first step in studying the replication initiation process in mycobacteria, we have isolated a 4-kb chromosomal DNA fragment from ...

journal_title：Journal of bacteriology

authors： Rajagopalan M,Qin MH,Nash DR,Madiraju MV

更新日期：1995-11-01 00:00:00

abstract：:Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, ...

journal_title：Journal of bacteriology

authors： Hien NH,Fleet GH

更新日期：1983-12-01 00:00:00

abstract：:Cells of Escherichia coli take up vitamin B(12) (cyano-cobalamin [CN-Cbl]) and iron chelates by use of sequential active transport processes. Transport of CN-Cbl across the outer membrane and its accumulation in the periplasm is mediated by the TonB-dependent transporter BtuB. Transport across the cytoplasmic membrane...

journal_title：Journal of bacteriology

authors： Cadieux N,Bradbeer C,Reeger-Schneider E,Köster W,Mohanty AK,Wiener MC,Kadner RJ

更新日期：2002-02-01 00:00:00

abstract：:By transformation of dnaA null mutant host cells that are suppressed either by an rnh mutation or by chromosomal integration of a mini-R1 plasmid, it was shown that replication of miniplasmids composed of the NR1 minimal replicon had no absolute dependence upon DnaA protein. In addition, the suppression of the dnaA nu...

journal_title：Journal of bacteriology

authors： Tang XB,Womble DD,Rownd RH

更新日期：1989-10-01 00:00:00

abstract：:Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

journal_title：Journal of bacteriology

authors： HOWARD DH

更新日期：1964-01-01 00:00:00

abstract：:A new insertion element, IS1549, was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isola...

journal_title：Journal of bacteriology

authors： Plikaytis BB,Crawford JT,Shinnick TM

更新日期：1998-03-01 00:00:00

abstract：:The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...

journal_title：Journal of bacteriology

authors： Campbell LA,Yasbin RE

更新日期：1984-10-01 00:00:00

abstract：:Pseudomonas aeruginosa and members of the Burkholderia cepacia complex often coexist in both the soil and the lungs of cystic fibrosis patients. To gain an understanding of how these different species affect each other's physiology when coexisting, we performed a screen to identify P. aeruginosa genes that are induced...

journal_title：Journal of bacteriology

authors： Weaver VB,Kolter R

更新日期：2004-04-01 00:00:00

abstract：:The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...

journal_title：Journal of bacteriology

authors： Cooper S,Weinberger M

更新日期：1977-04-01 00:00:00

abstract：:A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

journal_title：Journal of bacteriology

authors： Berg CM,Liu L,Wang B,Wang MD

更新日期：1988-01-01 00:00:00

abstract：UNLABELLED:Biofilm formation is responsible for increased antibiotic tolerance in pathogenic bacteria. Cyclic di-GMP (c-di-GMP) is a widely used second-messenger signal that plays a key role in bacterial biofilm formation. c-di-GMP is synthesized by diguanylate cyclases (DGCs), a conserved class of enzymes absent in ma...

journal_title：Journal of bacteriology

authors： Fernicola S,Paiardini A,Giardina G,Rampioni G,Leoni L,Cutruzzolà F,Rinaldo S

更新日期：2015-09-28 00:00:00