Abstract:
:Two replicons were isolated independently from different IncHI1 plasmids. One was isolated from R27, and a second was isolated from pIP522. We demonstrate, by DNA-DNA hybridization experiments, that these maintenance regions are different and that they are specific to, and carried by, all IncHI1 plasmids tested. In view of this specificity we decided to designate the replicon isolated from R27 as RepHI1A and the replicon isolated from pIP522 as RepHI1B. These two autoreplicative regions are not related to a third replicon present in all IncHI1 plasmids that bears homology with RepFIA and that expresses the characteristic incompatibility of IncHI1 subgroup plasmids toward F factor (D. Saul, D. Lane, and P. L. Bergquist, Mol. Microbiol. 2:219-225, 1988; D. E. Taylor, R. W. Hedges, and P. L. Bergquist, J. Gen. Microbiol. 131:1523-1530, 1985). These results demonstrate that all IncHI1 plasmids tested contain at least three replicons. An incompatibility (Inc) region that hybridizes specifically to all the IncHI1 plasmids was previously isolated (M. Couturier, F. Bex, P. L. Bergquist, and W. K. Maas, Microbiol. Rev. 52:375-395, 1988). Although this Inc locus is not located in an autoreplicative region of IncHI1 plasmids, we observed that this locus stabilizes a low-copy-number replicon. This Inc locus is probably a component of an active partition locus involved in the maintenance of IncHI1 plasmids. The nucleotide sequence of the Inc region contains direct repeats of 31 bp. In addition, this incompatibility determinant hybridizes specifically with IncHI1 plasmids but expresses incompatibility toward plasmids of both IncHI subgroups (IncHI1 and IncHI2). In this communication, we present the mapping of these maintenance elements on the R27 genome.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gabant P,Newnham P,Taylor D,Couturier Mdoi
10.1128/jb.175.23.7697-7701.1993subject
Has Abstractpub_date
1993-12-01 00:00:00pages
7697-701issue
23eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract:UNLABELLED:Silica is deposited in and around the spore coat layer of Bacillus cereus, and enhances the spore's acid resistance. Several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). Homologous sequence search revealed a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00447-15
更新日期:2015-10-26 00:00:00
abstract::The cell surfaces of several Lactobacillus species are covered by a regular layer composed of a single species of protein, the S-protein. The 43-kDa S-protein of the neotype strain Lactobacillus acidophilus ATCC 4356, which originated from the pharynx of a human, was purified. Antibodies generated against purified S-p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.19.6089-6096.1993
更新日期:1993-10-01 00:00:00
abstract::Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00237-11
更新日期:2011-05-01 00:00:00
abstract::A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.443-451.1983
更新日期:1983-04-01 00:00:00
abstract::Alysiella bovis adheres to surfaces by means of short, ruthenium red-staining, rod-like fimbriae. The fimbriae remain associated with the cell envelope of A. bovis, even when sonicated or exposed sequentially to toluene, Triton X-100, lysozyme, ribonuclease, and deoxyribonuclease. Adhesion of outer membrane-derived ce...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.569-576.1984
更新日期:1984-11-01 00:00:00
abstract::The electron transport system of Neisseria gonorrhoeae was partially characterized by using spectrophotometric, spectroscopic, and oxygen consumption measurements. The effects of selected electron transport inhibitors (amytal, rotenone, 2-heptyl-4-hydroxyquinoline, antimycin A1, and potassium cyanide [KCN]) on electro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.537-545.1978
更新日期:1978-05-01 00:00:00
abstract::Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.925-932.1985
更新日期:1985-09-01 00:00:00
abstract::D-Ribose isomerase was purified and crystallized from cells of Mycobacterium smegmatis grown on either D-ribose or L-rhamnose. Isomerase activity for both of these sugars remained together throughout the purification. The isomerase from L-rhamnose-grown cells had the same chemical and physical properties as the enzyme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.553-555.1976
更新日期:1976-04-01 00:00:00
abstract::Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.1.38-46.1965
更新日期:1965-07-01 00:00:00
abstract::The temperature-sensitive cyr1-2 mutant in Saccharomyces cerevisiae produces low levels of adenylate cyclase and cyclic AMP at 25 degrees C and is unable to synthesize repressible acid phosphatase at 25 degrees C. Suppressor mutants of cyr1-2 were isolated by detecting acid phosphatase activity. One of the dominant su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.7.2406-2408.1991
更新日期:1991-04-01 00:00:00
abstract::Microorganisms and plants utilize two-component systems to regulate adaptive responses to changing environmental conditions. Sensor kinases detect stimuli and alter their autophosphorylation activity accordingly. Signal propagation occurs via the transfer of phosphoryl groups from upstream kinases to downstream respon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00089-20
更新日期:2020-07-09 00:00:00
abstract::The plasmids R1162 and pSC101 have origins of conjugative transfer (oriTs) and corresponding relaxases that are closely related. The oriTs are made up of a highly conserved core, where DNA is cleaved by the relaxase prior to transfer, and an inverted repeat that differs in size and sequence. We show that in each case ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.2.499-506.2006
更新日期:2006-01-01 00:00:00
abstract::The complete sequences of the RhsB and RhsC elements of Escherichia coli K-12 have been determined. These sequence data reveal a new repeated sequence, called H-rpt (Hinc repeat), which is distinct from the Rhs core repetition that is found in all five Rhs elements. H-rpt is found in RhsB, RhsC, and RhsE. Characteriza...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.2799-2808.1993
更新日期:1993-05-01 00:00:00
abstract::Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01843-07
更新日期:2008-05-01 00:00:00
abstract::The inability of Micrococcus sodonensis to grow on glucose as the sole source of carbon and energy was investigated. Estimation of pathways of glucose catabolism indicated that both the glycolytic and hexose monophosphate pathways are present in this organism. Comparative studies with Escherichia coli demonstrated tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.6.1839-1846.1967
更新日期:1967-06-01 00:00:00
abstract::Mutations in the spoIIIA and spoIIIJ loci disrupt the compartmentalization of gene expression during sporulation of Bacillus subtilis. The breakdown in compartmentalization is not the cause of their being blocked in spore formation. Rather, it appears to be a consequence of the engulfed prespore's being unstable. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.2221-2223.2003
更新日期:2004-04-01 00:00:00
abstract::Flac mutants insensitive to transfer inhibition by R factors. JR66a and R485 were isolated and characterized. Representative mutations were cis dominant and are therefore presumed to be at the sites of action, fisU and fisV, respectively, of the FinU and FinV transfer inhibition systems encoded by JR66a and R485. The ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.2.413-420.1977
更新日期:1977-08-01 00:00:00
abstract::Insertion of Tn7 in nine independently derived isolates occurred in a single small region of the Caulobacter crescentus chromosome, suggesting that Tn7 insertion occurs at or near a single site. The order of markers in this region of the Caulobacter chromosome was shown to be flaA-argG-Tn7. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.1056-1058.1982
更新日期:1982-08-01 00:00:00
abstract:UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00250-16
更新日期:2016-05-27 00:00:00
abstract::A gene which complements a paralyzed flagellar mutant of Rhodobacter sphaeroides was sequenced. The derived protein sequence has similarity to MotB. R. sphaeroides MotB lacks the C-terminal peptidoglycan-binding motif of other MotB proteins. This divergence of sequence may reflect the unusual, unidirectional, stop-sta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2929-2932.1995
更新日期:1995-05-01 00:00:00
abstract::A positive selection method was used to isolate four Saccharomyces cerevisiae mutations that cause derepressed synthesis of asparaginase II. The four mutations (and1, and2, and3, and4) were neither closely linked to each other nor linked to previously characterized mutations (asp3, asp6) which cause the complete loss ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.1.293-296.1986
更新日期:1986-01-01 00:00:00
abstract::Beppu, Michiko (University of Tokyo, Tokyo, Japan), and Kei Arima. Decreased permeability as the mechanism of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:151-157. 1964.-The mechanism of arsenite resistance of Pseudomonas pseudomallei strain 54, isolated from soil, was studied by use of radioactiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.151-157.1964
更新日期:1964-07-01 00:00:00
abstract::Bacillus subtilis grows under anaerobic conditions utilizing nitrate ammonification and various fermentative processes. The two-component regulatory system ResDE and the redox regulator Fnr are the currently known parts of the regulatory system for anaerobic adaptation. Mutation of the open reading frame ywiD located ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.23.6815-6821.2001
更新日期:2001-12-01 00:00:00
abstract::The structural genes of ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) from Salmonella typhimurium LT2 were cloned on a 5.8-kilobase-pair insert in the SalI site of pBR322. A single strand specific radioactive probe containing the N terminus of the Escherichia coli K-12 glgC gene in M13mp8 was used t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4349-4354.1987
更新日期:1987-09-01 00:00:00
abstract::The Escherichia coli structural gene for glutathione synthetase, gshB, was cloned into pBR322. Plasmids containing gshB were able to complement the glutathione requirement of a trxA gshB double mutant, and cells containing the plasmids were found to have elevated levels of glutathione synthetase. A mutant gshB allele ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.5218-5221.1989
更新日期:1989-09-01 00:00:00
abstract::The utilization of glycerol as a carbon source for growth by Klebsiella aerogenes, strain 2103, involves separate aerobic (sn-glycerol-3-phosphate or G3P) and anaerobic (dihydroxyacetone or DHA) pathways of catabolism. Enzyme and transport activities of the aerobic pathway are elevated in cells grown under oxygenated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.1.50-56.1974
更新日期:1974-07-01 00:00:00
abstract::RSF1010 plasmid DNA was introduced into Pseudomonas putida and P. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in Pseudomonas species. The number of copies of RSF1010 was 43 per chromosome equivalent in P. putida cells. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1527-1529.1978
更新日期:1978-03-01 00:00:00
abstract::We screened mini-Mu plasmid libraries from Enterobacter aerogenes IFO 12010 for plasmids that complement Escherichia coli phn mutants that cannot use phosphonates (Pn) as the sole source of phosphorus (P). We isolated two kinds of plasmids that, unexpectedly, encode genes for different metabolic pathways. One kind com...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2501-2510.1992
更新日期:1992-04-01 00:00:00
abstract::Three Escherichia coli phages, TuIa, TuIb, and TuII, were isolated from local sewage. We present evidence that they use the major outer membrane proteins Ia, Ib, and II, respectively, as receptors. In all cases the proteins, under the experimental conditions used, required lipopolysaccharide to exhibit their receptor ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.3.821-829.1977
更新日期:1977-09-01 00:00:00
abstract::Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.755-760.1983
更新日期:1983-08-01 00:00:00