Further characterization of the F fertility inhibition systems of "unusual" Fin+ plasmids.

Abstract:

:Flac mutants insensitive to transfer inhibition by R factors. JR66a and R485 were isolated and characterized. Representative mutations were cis dominant and are therefore presumed to be at the sites of action, fisU and fisV, respectively, of the FinU and FinV transfer inhibition systems encoded by JR66a and R485. The mutants were used to confirm that the FinU and FinV fertility inhibition systems are different from each other and from the FinOP, FinQ, and FinW systems of R100, R62, and R455, respectively. Together with traO and fisQ mutants of Flac, the new mutants were also used to investigate the nature of the F fertility inhibition systems encoded by a further group of "unusual" Fin+ plasmids. Of these, two incompatibility group X plasmids were found to carry finO+ genes, and of five incompatibility group I plasmids, three encoded FinQ systems, one the FinU system, and one a new system (FinR). Transfer of a variety of derepressed F-like plasmids was inhibited by the FinQ, FinU, and FinV systems, but a quantitatively very different levels; this emphasizes the differences as well as the similarities between the conjugation systems of F-like plasmids.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gasson MJ,Willetts NS

doi

10.1128/JB.131.2.413-420.1977

subject

Has Abstract

pub_date

1977-08-01 00:00:00

pages

413-20

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

131

pub_type

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