Evidence that the isomerization of D-ribose and L-rhamnose is catalyzed by the same enzyme in Mycobacterium smegmatis.

Abstract:

:D-Ribose isomerase was purified and crystallized from cells of Mycobacterium smegmatis grown on either D-ribose or L-rhamnose. Isomerase activity for both of these sugars remained together throughout the purification. The isomerase from L-rhamnose-grown cells had the same chemical and physical properties as the enzyme isolated from D-ribose grown cells. In addition, immunological studies indicated that both activities were in the same protein since antisera prepared against either of the crystals cross-reacted with the other and gave lines of symmetry by the agar gel diffusion method.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Izumori K,Mitchell M,Elbein AD

doi

10.1128/JB.126.1.553-555.1976

keywords:

subject

Has Abstract

pub_date

1976-04-01 00:00:00

pages

553-5

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

126

pub_type

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