Homology among Escherichia coli K1 and K92 polysialytransferases.

abstract：:A reaction center H- strain (RCH-) of Rhodobacter sphaeroides, PUHA1, was made by in vitro deletion of an XhoI restriction endonuclease fragment from the puhA gene coupled with insertion of a kanamycin resistance gene cartridge. The resulting construct was delivered to R. sphaeroides wild-type 2.4.1, with the defectiv...

journal_title：Journal of bacteriology

authors： Sockett RE,Donohue TJ,Varga AR,Kaplan S

更新日期：1989-01-01 00:00:00

abstract：:The polar pili of Pseudomonas aeruginosa are composed of subunits encoded by the pilA gene. Expression of pilA requires the alternative sigma factor RpoN and a pair of regulatory elements, PilS and PilR. These two proteins are members of the two-component regulatory family, in which PilS is the sensory component and P...

journal_title：Journal of bacteriology

authors： Boyd JM,Lory S

更新日期：1996-02-01 00:00:00

abstract：:Alcaligenes sp. strain CC1 is able to grow on several alpha-chlorinated aliphatic acids (2-chlorobutyrate, 2-chloropropionate, and chloroacetate), as well as on the beta-chlorinated four-carbon aliphatic acids trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate as sole carbon and energy sources. Dehal...

journal_title：Journal of bacteriology

authors： Kohler-Staub D,Kohler HP

更新日期：1989-03-01 00:00:00

abstract：:Pediococcus halophilus X-160 which lacks catabolite control by glucose was isolated from nature (soy moromi mash). Wild-type strains, in xylose-glucose medium, utilized glucose preferentially over xylose and showed diauxic growth. With wild-type strain I-13, xylose isomerase activity was not induced until glucose was ...

journal_title：Journal of bacteriology

authors： Abe K,Uchida K

更新日期：1989-04-01 00:00:00

abstract：:Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...

journal_title：Journal of bacteriology

authors： de Vries N,Duinsbergen D,Kuipers EJ,Pot RG,Wiesenekker P,Penn CW,van Vliet AH,Vandenbroucke-Grauls CM,Kusters JG

更新日期：2002-12-01 00:00:00

abstract：:Products of the approximately 9.5-kb virB operon are proposed to direct the export of T-DNA/protein complexes across the Agrobacterium tumefaciens envelope en route to plant cells. The presence of conserved nucleoside triphosphate (NTP)-binding domains in VirB4 and VirB11 suggests that one or both proteins couple ener...

journal_title：Journal of bacteriology

authors： Berger BR,Christie PJ

更新日期：1993-03-01 00:00:00

abstract：:Bacillus subtilis produces a 35-kDa cell wall hydrolase, CwlF, during vegetative growth. The CwlF protein was extracted from B. subtilis cwlB sigD mutant cells and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequencing revealed that its sequence is completely identical...

journal_title：Journal of bacteriology

authors： Ishikawa S,Hara Y,Ohnishi R,Sekiguchi J

更新日期：1998-05-01 00:00:00

abstract：:Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...

journal_title：Journal of bacteriology

authors： Bierne H,Garandeau C,Pucciarelli MG,Sabet C,Newton S,Garcia-del Portillo F,Cossart P,Charbit A

更新日期：2004-04-01 00:00:00

abstract：:The plasmids R1162 and pSC101 have origins of conjugative transfer (oriTs) and corresponding relaxases that are closely related. The oriTs are made up of a highly conserved core, where DNA is cleaved by the relaxase prior to transfer, and an inverted repeat that differs in size and sequence. We show that in each case ...

journal_title：Journal of bacteriology

authors： Jandle S,Meyer R

更新日期：2006-01-01 00:00:00

abstract：:The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and ...

journal_title：Journal of bacteriology

authors： Legrand P,Bouche JP,Louarn JM

更新日期：1979-12-01 00:00:00

abstract：:Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, ...

journal_title：Journal of bacteriology

authors： Bodenmiller DM,Spiro S

更新日期：2006-02-01 00:00:00

abstract：:The polycistronic mRNA encoding the nine genes of the unc operon of Escherichia coli was studied. We demonstrated the ribosome-binding capabilities of six of the nine unc genes, uncB, uncE, uncF, uncH, uncA, and uncD, by using the technique of primer extension inhibition or "toeprinting." No toeprint was detected for ...

journal_title：Journal of bacteriology

authors： Schaefer EM,Hartz D,Gold L,Simoni RD

更新日期：1989-07-01 00:00:00

abstract：:The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...

journal_title：Journal of bacteriology

authors： O'Connor M,Gregory ST

更新日期：2011-01-01 00:00:00

abstract：:After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

journal_title：Journal of bacteriology

authors： Friedman SM,Axel R,Weinstein IB

更新日期：1967-05-01 00:00:00

abstract：:The organization and transcriptional control of chromosomal cat genes (required for dissimilation of catechol by the beta-ketoadipate pathway) in the Pseudomonas putida biotype strain (ATCC 12633) are reported. Nucleotide sequence reveals that catR is separated by 135 bp from the divergently transcribed catBC,A; catC ...

journal_title：Journal of bacteriology

authors： Houghton JE,Brown TM,Appel AJ,Hughes EJ,Ornston LN

更新日期：1995-01-01 00:00:00

abstract：UNLABELLED:The 2-thiouridine (s(2)U) modification of the wobble position in glutamate, glutamine, and lysine tRNA molecules serves to stabilize the anticodon structure, improving ribosomal binding and overall efficiency of the translational process. Biosynthesis of s(2)U in Escherichia coli requires a cysteine desulfur...

journal_title：Journal of bacteriology

authors： Black KA,Dos Santos PC

更新日期：2015-06-01 00:00:00

abstract：:We examined the kinetics of beta-galactosidase (EC 3.2.1.23) induction in the yeast Kluyveromyces lactis. Enzyme activity began to increase 10 to 15 min, about 1/10 of a cell generation, after the addition of inducer and continued to increase linearly for from 7 to 9 cell generations before reaching a maximum, some 12...

journal_title：Journal of bacteriology

authors： Dickson RC,Markin JS

更新日期：1980-06-01 00:00:00

abstract：:Polyamines are present in high concentrations in archaea, yet little is known about their synthesis, except by extrapolation from bacterial and eucaryal systems. S-Adenosylmethionine (AdoMet) decarboxylase, a pyruvoyl group-containing enzyme that is required for spermidine biosynthesis, has been previously identified ...

journal_title：Journal of bacteriology

authors： Kim AD,Graham DE,Seeholzer SH,Markham GD

更新日期：2000-12-01 00:00:00

abstract：:The location of the Escherichia coli K-12 genes determining or regulating glutamate transport, and the location of the gene determining glutamate decarboxylase synthesis, were established by conjugation. The ability to grow on glutamate as the sole source of carbon and energy was used to select for glutamate transport...

journal_title：Journal of bacteriology

authors： Marcus M,Halpern YS

更新日期：1967-04-01 00:00:00

abstract：:Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

journal_title：Journal of bacteriology

authors： Caffrey P,Owen P

更新日期：1989-07-01 00:00:00

abstract：:Accumulation of K+ ions and glutamate plays a primary role in maintaining osmotic balance in Escherichia coli, as illustrated by the high concentrations of these ions present in cells growing in medium of high osmolality. We found that two gamma-glutamyl peptides and glutamine also accumulated during growth at high os...

journal_title：Journal of bacteriology

authors： McLaggan D,Logan TM,Lynn DG,Epstein W

更新日期：1990-07-01 00:00:00

abstract：:Horizontal gene transfer by conjugation plays a major role in bacterial evolution, allowing the acquisition of new traits, such as virulence and resistance to antibacterial agents. With the increased antibiotic resistance in bacterial pathogens, a better understanding of how bacteria modulate conjugation under changin...

journal_title：Journal of bacteriology

authors： Grace ED,Gopalkrishnan S,Girard ME,Blankschien MD,Ross W,Gourse RL,Herman C

更新日期：2015-03-01 00:00:00

abstract：:Lipoprotein biogenesis in Gram-negative bacteria occurs by a conserved pathway, each step of which is considered essential. In contrast to this model, LoVullo and colleagues demonstrate that the N-acyl transferase Lnt is not required in Francisella tularensis or Neisseria gonorrhoeae. This suggests the existence of a ...

journal_title：Journal of bacteriology

authors： Chahales P,Thanassi DG

更新日期：2015-05-01 00:00:00

abstract：:Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophor...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schnaitman CA

更新日期：1978-03-01 00:00:00

abstract：:The growth constant and Y (sucrose) (grams of cells per mole of sucrose) for NH(3)-grown cultures of Clostridium pasteurianum were 1.7 times those of N(2)-grown cultures, whereas the rate of sucrose utilized per gram of cells per hour was similar for both conditions. The Y (sucrose) of chemostat cultures grown on limi...

journal_title：Journal of bacteriology

authors： Daesch G,Mortenson LE

更新日期：1968-08-01 00:00:00

abstract：:Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...

journal_title：Journal of bacteriology

authors： Cybulska J,Jeljaszewicz J

更新日期：1966-03-01 00:00:00

abstract：:The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional replication origins in Escherichia coli. The origins in the SalI restriction fragments of 17.5 and 10.2 kilobase pairs, cloned from E. aer...

journal_title：Journal of bacteriology

authors： Harding NE,Cleary JM,Smith DW,Michon JJ,Brusilow WS,Zyskind JW

更新日期：1982-12-01 00:00:00

abstract：:The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

journal_title：Journal of bacteriology

authors： LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

更新日期：2003-11-01 00:00:00

abstract：:A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...

journal_title：Journal of bacteriology

authors： Warner JR,Morgan SA,Shulman RW

更新日期：1976-03-01 00:00:00

abstract：:The imp genes, responsible for the UV protection and mutation effects of the I incompatibility group plasmid TP110, have been cloned into vector plasmids, and their products have been analyzed. The genetic information required for expression of these properties was carried in a continuous DNA sequence of approximately...

journal_title：Journal of bacteriology

authors： Glazebrook JA,Grewal KK,Strike P

更新日期：1986-10-01 00:00:00