Physiological studies of beta-galactosidase induction in Kluyveromyces lactis.

abstract：:Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for huma...

journal_title：Journal of bacteriology

authors： Cohen IR

更新日期：1967-07-01 00:00:00

abstract：:The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple o...

journal_title：Journal of bacteriology

authors： Simpson KH,Bowden G,Höök M,Anvari B

更新日期：2003-03-01 00:00:00

abstract：:DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...

journal_title：Journal of bacteriology

authors： Vujaklija D,Horinouchi S,Beppu T

更新日期：1993-05-01 00:00:00

abstract：:Wolin, M. J. (University of Illinois, Urbana). Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bacteriol. 91:1781-1786. 1966.-Cell suspensions of Vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (EDTA) or lysozyme. Lysis occurs at alkaline pH and is prevented by 0.15 m N...

journal_title：Journal of bacteriology

authors： Wolin MJ

更新日期：1966-05-01 00:00:00

abstract：:The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derive...

journal_title：Journal of bacteriology

authors： Ke D,Boissinot M,Huletsky A,Picard FJ,Frenette J,Ouellette M,Roy PH,Bergeron MG

更新日期：2000-12-01 00:00:00

abstract：:The bacterium Rhodobacter capsulatus is capable of an unusual form of genetic exchange, mediated by a transducing bacteriophage-like particle called the gene transfer agent (GTA). GTA production by R. capsulatus is controlled at the level of transcription by a cellular two-component signal transduction system that inc...

journal_title：Journal of bacteriology

authors： Lang AS,Beatty JT

更新日期：2002-02-01 00:00:00

abstract：:Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activiti...

journal_title：Journal of bacteriology

authors： Habe H,Chung JS,Kato H,Ayabe Y,Kasuga K,Yoshida T,Nojiri H,Yamane H,Omori T

更新日期：2004-09-01 00:00:00

abstract：:Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...

journal_title：Journal of bacteriology

authors： Kobayashi K

更新日期：2007-07-01 00:00:00

abstract：:Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...

journal_title：Journal of bacteriology

authors： Vales LD,Chase JW,Murphy JB

更新日期：1979-07-01 00:00:00

abstract：:Bacillus subtilis JH642 and a wild strain of B. subtilis called 22a both produce an antilisterial peptide that can be purified by anion-exchange and gel filtration chromatography. Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. A mutant defective in production of the substance w...

journal_title：Journal of bacteriology

authors： Zheng G,Yan LZ,Vederas JC,Zuber P

更新日期：1999-12-01 00:00:00

abstract：:Agrobacterium tumefaciens transfers single-stranded DNAs (T strands) into plant cells. VirE1 and VirE2, which is a single-stranded DNA binding protein, are important for tumorigenesis. We show that T strands and VirE2 can enter plant cells independently and that export of VirE2, but not of T strands, depends on VirE1....

journal_title：Journal of bacteriology

authors： Sundberg C,Meek L,Carroll K,Das A,Ream W

更新日期：1996-02-01 00:00:00

abstract：:A chemotaxis signal transduction pathway (hereafter called Che1) has been previously identified in the alphaproteobacterium Azospirillum brasilense. Previous experiments have demonstrated that although mutants lacking CheB and/or CheR homologs from this pathway are defective in chemotaxis, a mutant in which the entire...

journal_title：Journal of bacteriology

authors： Bible AN,Stephens BB,Ortega DR,Xie Z,Alexandre G

更新日期：2008-10-01 00:00:00

abstract：:The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...

journal_title：Journal of bacteriology

authors： Chitlaru T,Gat O,Gozlan Y,Ariel N,Shafferman A

更新日期：2006-05-01 00:00:00

abstract：:Bacterial pathogenesis depends on changes in metabolic and virulence gene expression in response to changes within a pathogen's environment. The plague-causing pathogen, Yersinia pestis, requires expression of the gene encoding the Pla protease for progression of pneumonic plague. The catabolite repressor protein Crp,...

journal_title：Journal of bacteriology

authors： Ritzert JT,Lathem WW

更新日期：2018-05-09 00:00:00

abstract：:The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...

journal_title：Journal of bacteriology

authors： Kew OM,Douglas HC

更新日期：1976-01-01 00:00:00

abstract：:The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...

journal_title：Journal of bacteriology

authors： Rosta S,Mett H

更新日期：1989-01-01 00:00:00

abstract：:Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...

journal_title：Journal of bacteriology

authors： Dubourdieu M,DeMoss JA

更新日期：1992-02-01 00:00:00

abstract：:Tryptophan auxotrophs of the archaebacterium Haloferax volcanii define a cluster of overlapping genes homologous to eubacterial-eukaryotic trpD, -F, -E, and -G, linked in that order and each preceded by a possible ribosome binding site. Residues involved in feedback inhibition of eubacterial anthranilate synthetases a...

journal_title：Journal of bacteriology

authors： Lam WL,Logan SM,Doolittle WF

更新日期：1992-03-01 00:00:00

abstract：:The ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the protein...

journal_title：Journal of bacteriology

authors： Sun TT,Bickle TA,Traut RR

更新日期：1972-08-01 00:00:00

abstract：:Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...

journal_title：Journal of bacteriology

authors： Summers RG,Wendt-Pienkowski E,Motamedi H,Hutchinson CR

更新日期：1992-03-01 00:00:00

abstract：:Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid during sporulation. Other spore structures appeared normal. Spore shape was quite irregular in the absence of meso-alpha,ep...

journal_title：Journal of bacteriology

authors： Imae Y,Strominger MB,Strominger JL

更新日期：1976-09-01 00:00:00

abstract：:Phosphoenolypyruvate-dependent maltose:phosphotransferase activity was induced in cells of Fusobacterium mortiferum ATCC 25557 during growth on maltose. The disaccharide was rapidly metabolized by washed cells maintained under anaerobic conditions, but fermentation ceased immediately upon exposure of the cell suspensi...

journal_title：Journal of bacteriology

authors： Robrish SA,Fales HM,Gentry-Weeks C,Thompson J

更新日期：1994-06-01 00:00:00

abstract：:Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...

journal_title：Journal of bacteriology

authors： Hamrick TS,Harris SL,Spears PA,Havell EA,Horton JR,Russell PW,Orndorff PE

更新日期：2000-07-01 00:00:00

abstract：:The three-component naphthalene dioxygenase (NDO) enzyme system carries out the first step in the aerobic degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The three-dimensional structure of NDO revealed that several of the amino acids at the active site of the oxygenase are hydrophobic, which is consi...

journal_title：Journal of bacteriology

authors： Parales RE,Lee K,Resnick SM,Jiang H,Lessner DJ,Gibson DT

更新日期：2000-03-01 00:00:00

abstract：:We have cloned the Micromonospora viridifaciens neuraminidase (EC 3.2.1.18) gene (nedA) in Streptomyces lividans. This was accomplished by using the vector pIJ702 and BglII-BclI libraries of M. viridifaciens chromosomal inserts created in S. lividans. The libraries were screened for the expression of neuraminidase by ...

journal_title：Journal of bacteriology

authors： Sakurada K,Ohta T,Hasegawa M

更新日期：1992-11-01 00:00:00

abstract：:The methanol-insoluble, heat-stable enterotoxin of Escherichia coli synthesized by clinical strains or strains that harbor the cloned gene was shown to be an extracellular polypeptide. The toxin (STB) was first detected as an 8,100-Mr precursor (pre-STB) that was converted to a transiently cell-associated 5,200-Mr for...

journal_title：Journal of bacteriology

authors： Kupersztoch YM,Tachias K,Moomaw CR,Dreyfus LA,Urban R,Slaughter C,Whipp S

更新日期：1990-05-01 00:00:00

abstract：:Escherichia coli has a formate hydrogenlyase system which allows it to maintain an electron balance during anaerobic growth by passing electrons from formate to H+ ions, thus generating H2. The Mu d1(Ap lac) bacteriophage was used to generate mutants that were defective in passing electrons from formate to benzyl viol...

journal_title：Journal of bacteriology

authors： Yerkes JH,Casson LP,Honkanen AK,Walker GC

更新日期：1984-04-01 00:00:00

abstract：:The DNAs of strains of three cyanobacterial genera (Anabaena, Plectonema, and Synechococcus) were found to be partially or fully resistant to many restriction endonucleases. This could be due to the absence of specific sequences or to modifications, rendering given sequences resistant to cleavage. The latter explanati...

journal_title：Journal of bacteriology

authors： Padhy RN,Hottat FG,Coene MM,Hoet PP

更新日期：1988-04-01 00:00:00

abstract：:The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates o...

journal_title：Journal of bacteriology

authors： Lambert MP,Neuhaus FC

更新日期：1972-06-01 00:00:00

abstract：:A P22 hybrid phage bearing the bacteriophage T4 lysozyme gene (e), as well as T4 sequences upstream from the lysozyme gene, was constructed. Amber mutations were introduced into gene e in the hybrid phage, and the resulting mutant phages were tested for the ability to form plaques on amber suppressor strains. Revertan...

journal_title：Journal of bacteriology

authors： Knight JA,Hardy LW,Rennell D,Herrick D,Poteete AR

更新日期：1987-10-01 00:00:00