Abstract:
:Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for human IgG, IgA, or IgM in the IFA procedure. The effects of heat and mercaptoethanol on IFA reactivities were also studied. It appeared that antibodies of the IgG, IgM, and IgA classes present in the sera of both infected persons (immune antibodies) and normal persons with no history of gonococcal infection (natural antibodies) react with heat-stable somatic antigens. Immune IgG antibodies, however, were distinguishable from natural IgG antibodies by their ability to recognize heat-labile surface antigens. The distinction between natural and immune IgM antibodies was less obvious. IgM antibodies from both infected and normal persons appeared to react with heat-labile antigens. Some, but not all, infected persons had immune IgA antibodies to heat-labile as well as to heat-stable antigens. Treatment of sera with mercaptoethanol had no effect on IgG antibodies. The IFA activity of IgM antibodies was decreased, but not abolished. The effects of mercaptoethanol on IgA antibodies were variable. Some sera showed a decrease in IgA titer, and others showed an increase in IgA activity to certain antigens. Immune IgG antibodies were more resistant to heating than were natural IgG antibodies. Natural and immune IgM antibodies appeared equally sensitive to heating. IgA activity, on the other hand, was increased by heating sera at 60 C, but was decreased at higher temperatures. Thus, it appears that natural and immune human IgG antibodies to N. gonorrhoeae may be distinguished by their interactions with heat-labile antigens and by their resistance to heating.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cohen IRdoi
10.1128/JB.94.1.141-148.1967subject
Has Abstractpub_date
1967-07-01 00:00:00pages
141-8issue
1eissn
0021-9193issn
1098-5530journal_volume
94pub_type
杂志文章abstract::Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.14.3578-3583.1998
更新日期:1998-07-01 00:00:00
abstract::Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.805-807.1984
更新日期:1984-08-01 00:00:00
abstract::The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system (T3SS) to evade phagocytosis and damage eukaryotic cells. Transcription of the T3SS regulon is controlled by ExsA, a member of the AraC/XylS family of transcriptional regulators. These family members generally consist of an approxim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00002-09
更新日期:2009-06-01 00:00:00
abstract::A new alginate regulatory gene, algQ, was identified in a chromosomal region which, when tandemly amplified, induces mucoidy in Pseudomonas aeruginosa. The algQ gene was found closely linked to the previously identified algR gene. Both algQ and algR were required for transcription of the key alginate biosynthetic gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3680-3688.1989
更新日期:1989-07-01 00:00:00
abstract::The CheA kinase is a central protein in the signal transduction network that controls chemotaxis in Escherichia coli. CheA receives information from a transmembrane receptor (e.g., Tar) and CheW proteins and relays it to the CheB and CheY proteins. The biochemical activities of CheA proteins truncated at various dista...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.2097-2101.1993
更新日期:1993-04-01 00:00:00
abstract::Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PE...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00508-08
更新日期:2008-09-01 00:00:00
abstract::O antigen is the major cell surface antigen of gram-negative bacteria, and the genes responsible for its synthesis are located in a single gene cluster. The wzx (rbfX) gene, which is characteristic of the major class of O-antigen gene clusters, encodes a hydrophobic protein with 12 potential transmembrane segments. We...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.2102-2107.1996
更新日期:1996-04-01 00:00:00
abstract::Two sensor proteins, NarX and NarQ, mediate nitrate regulation of anaerobic respiratory gene expression. Either of these sensors is sufficient to signal the presence of nitrate to the response regulator protein, NarL, a transcriptional activator and repressor. Two observations suggested the existence of a second respo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.11.3259-3268.1993
更新日期:1993-06-01 00:00:00
abstract::The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1062-1070.1983
更新日期:1983-09-01 00:00:00
abstract::Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1977-1985.1966
更新日期:1966-05-01 00:00:00
abstract::The ompA gene of Escherichia coli codes for a major protein of the outer membrane. When this gene was moved between various unrelated strains (E. coli K-12 and two clinical isolates of E. coli) by transduction, the gene was expressed very poorly. Recombinants carrying "foreign" genes produced no OmpA protein which cou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.403-410.1980
更新日期:1980-07-01 00:00:00
abstract::A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were prev...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.286-290.1968
更新日期:1968-02-01 00:00:00
abstract::Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1697-1705.1967
更新日期:1967-11-01 00:00:00
abstract::The bacteriophage P22-based challenge-phage system was used to study the binding of Xis and FIS to their sites in attP of bacteriophage lambda. Challenge phages were constructed that contained the X1, X2, and F sites within the P22 Pant promoter, which is required for expression of antirepressor. If Xis and FIS bind t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.19.5954-5963.1991
更新日期:1991-10-01 00:00:00
abstract::The activation properties of the form I and form II ribulose 1,5-bisphosphate carboxylases from Rhodopseudomonas sphaeroides were examined. Both enzymes have a requirement of Mg2+ for optimal activity. Mn2+, Ni2+, and Co2+ can also support activity of the form I enzyme, whereas only Mn2+ can substitute for Mg2+ with t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1023-1027.1979
更新日期:1979-12-01 00:00:00
abstract::Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.867-872.1983
更新日期:1983-11-01 00:00:00
abstract::Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00182-06
更新日期:2006-08-01 00:00:00
abstract::Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from hu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00222-09
更新日期:2009-06-01 00:00:00
abstract::Elevation of active sigma(E) levels in Escherichia coli by either repressing the expression of rseA encoding an anti-sigma(E) factor or cloning rpoE in a multicopy plasmid, led to a large decrease in the number of dead cells and the accumulation of cellular proteins in the medium in the stationary phase. The numbers o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.18.5231-5237.2000
更新日期:2000-09-01 00:00:00
abstract::Mycoplasma fermentans is a microorganism commonly found in the genitourinary and respiratory tracts of healthy individuals and AIDS patients. The complete genome of the repetitive-sequence-rich M. fermentans strain M64 is reported here. Comparative genomics analysis revealed dramatic differences in genome size between...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05228-11
更新日期:2011-08-01 00:00:00
abstract::We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2478-2484.1992
更新日期:1992-04-01 00:00:00
abstract::The location of the Escherichia coli K-12 genes determining or regulating glutamate transport, and the location of the gene determining glutamate decarboxylase synthesis, were established by conjugation. The ability to grow on glutamate as the sole source of carbon and energy was used to select for glutamate transport...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.4.1409-1415.1967
更新日期:1967-04-01 00:00:00
abstract::Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.375-379.1965
更新日期:1965-08-01 00:00:00
abstract::Heat, salinity, or osmotic stress influenced protein synthesis in nitrogen-fixing Anabaena sp. strain L-31. Salinity and osmotic stresses were identical and specifically induced 15 polypeptides. Four polypeptides were unique to heat shock, and four other polypeptides were induced under every stress. The results demons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.5187-5189.1989
更新日期:1989-09-01 00:00:00
abstract::The chlorosome envelope of Chlorobaculum tepidum contains 10 proteins that belong to four structural motif families. A previous mutational study (N.-U. Frigaard, H. Li, K. J. Milks, and D. A. Bryant, J. Bacteriol. 186:646-653, 2004) suggested that some of these proteins might have redundant functions. Six multilocus m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00707-09
更新日期:2009-11-01 00:00:00
abstract::Glutamine phosphoribosylpyrophosphate amidotransferase is stable in growing cells, but is inactivated in an oxygen-dependent process at various rates in starving or antibiotic-treated cells. On the basis of studies of the purified enzyme, we suggested (D.A. Bernlohr and R.L. Switzer, Biochemistry 20:5675-5681, 1981) t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.937-949.1983
更新日期:1983-02-01 00:00:00
abstract:UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00189-15
更新日期:2015-07-01 00:00:00
abstract::Myxococcus xanthus, a gram-negative bacterium, has a complex life cycle. In response to starvation, most cells in a population participate in the formation of multicellular aggregates (i.e., fruiting bodies) in which cells differentiate into spores. However, some cells do not enter aggregates. In this and the two acco...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.11.3318-3333.1991
更新日期:1991-06-01 00:00:00
abstract::The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01722-07
更新日期:2008-04-01 00:00:00
abstract::It is thought that in the gram-negative soil bacterium Sinorhizobium meliloti the protein ExoP is involved in biosynthesis of the acidic exopolysaccharide succinoglycan (EPS I). The amounts and compositions of EPS I produced by mutants expressing ExoP proteins characterized by specific amino acid substitutions in the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.17.5163-5170.2001
更新日期:2001-09-01 00:00:00