Detergent-resistant Streptococcus faecium derivatives that display conditional penicillin lysis.

abstract：:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

journal_title：Journal of bacteriology

authors： Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

更新日期：2008-03-01 00:00:00

abstract：:Welker, N. E. (Western Reserve University, Cleveland, Ohio and University of Illinois, Urbana), and L. Leon Campbell. De novo synthesis of alpha-amylase by Bacillus stearothermophilus. J. Bacteriol. 86:1202-1210. 1963.-The pH optimum for the synthesis of alpha-amylase by washed-cell suspensions was 6.7. alpha-Amylase ...

journal_title：Journal of bacteriology

authors： WELKER NE,CAMPBELL LL

更新日期：1963-12-01 00:00:00

abstract：:Penicillin-binding proteins of 180, 89, 80, 68, 61, 41, and 38 kilodaltons were identified in Treponema pallidum (Nichols) by their covalent binding of [35S]benzylpenicillin. Penicillin-binding proteins are localized in the plasma membranes of many bacterial species and may serve as useful markers for determining plas...

journal_title：Journal of bacteriology

authors： Cunningham TM,Miller JN,Lovett MA

更新日期：1987-11-01 00:00:00

abstract：:A total of 508 random clones from five Mycoplasma genitalium genomic libraries were partially sequenced and analyzed. This resulted in the identification of 291 unique contigs. Sequence information from these clones (100,993 nucleotides), representing approximately 17% of this pathogen's genome, was analyzed by compar...

journal_title：Journal of bacteriology

authors： Peterson SN,Hu PC,Bott KF,Hutchison CA 3rd

更新日期：1993-12-01 00:00:00

abstract：:Spx is a global regulator that is widespread among the low-G+C-content gram-positive bacteria. Spx has been extensively studied in Bacillus subtilis, where it acts as an activator and a repressor of transcription in response to disulfide stress. Under nonstress conditions, Spx is rapidly degraded by the ClpXP protease...

journal_title：Journal of bacteriology

authors： Engman J,Rogstam A,Frees D,Ingmer H,von Wachenfeldt C

更新日期：2012-03-01 00:00:00

abstract：:The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...

journal_title：Journal of bacteriology

authors： Sherman MB,Orlova EV,Smirnova EA,Hovmöller S,Zorin NA

更新日期：1991-04-01 00:00:00

abstract：:The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

journal_title：Journal of bacteriology

authors： LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

更新日期：2003-11-01 00:00:00

abstract：:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...

journal_title：Journal of bacteriology

authors： Previte JJ

更新日期：1968-06-01 00:00:00

abstract：:Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patter...

journal_title：Journal of bacteriology

authors： Dale JW,Al-Ghusein H,Al-Hashmi S,Butcher P,Dickens AL,Drobniewski F,Forbes KJ,Gillespie SH,Lamprecht D,McHugh TD,Pitman R,Rastogi N,Smith AT,Sola C,Yesilkaya H

更新日期：2003-04-01 00:00:00

abstract：:The pst gene cluster encodes the phosphate-specific transport (Pst) system. Inactivation of the Pst system constitutively activates the two-component regulatory system PhoBR and attenuates the virulence of pathogenic bacteria. In uropathogenic Escherichia coli strain CFT073, attenuation by inactivation of pst is predo...

journal_title：Journal of bacteriology

authors： Crépin S,Porcheron G,Houle S,Harel J,Dozois CM

更新日期：2017-11-14 00:00:00

abstract：:Mycobacterium tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for biosynthesis of isopentenyl diphosphate and its isomer, dimethylallyl diphosphate, precursors of all isoprenoid compounds. This pathway is of interest as a source of new drug targets, as it is absent from humans and disruption of the ...

journal_title：Journal of bacteriology

authors： Eoh H,Brown AC,Buetow L,Hunter WN,Parish T,Kaur D,Brennan PJ,Crick DC

更新日期：2007-12-01 00:00:00

abstract：:Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

journal_title：Journal of bacteriology

authors： Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

更新日期：1990-09-01 00:00:00

abstract：:The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

journal_title：Journal of bacteriology

authors： Griffiths MW,Sundaram TK

更新日期：1973-12-01 00:00:00

abstract：:A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) ...

journal_title：Journal of bacteriology

authors： Weisburg WG,Barns SM,Pelletier DA,Lane DJ

更新日期：1991-01-01 00:00:00

abstract：:Transcriptional factor CarD is the only reported prokaryotic analog of eukaryotic high-mobility-group A (HMGA) proteins, in that it has contiguous acidic and AT hook DNA-binding segments and multifunctional roles in Myxococcus xanthus carotenogenesis and fruiting body formation. HMGA proteins are small, randomly struc...

journal_title：Journal of bacteriology

authors： Cayuela ML,Elías-Arnanz M,Peñalver-Mellado M,Padmanabhan S,Murillo FJ

更新日期：2003-06-01 00:00:00

abstract：:The Saccharomyces cerevisiae gene MEC1 represents a structural homolog of the human gene ATM mutated in ataxia telangiectasia patients. Like human ataxia telangiectasia cell lines, mec1 mutants are defective in G2 and S-phase cell cycle checkpoints in response to radiation treatment. Here we show an additional defect ...

journal_title：Journal of bacteriology

authors： Siede W,Allen JB,Elledge SJ,Friedberg EC

更新日期：1996-10-01 00:00:00

abstract：:A mutant, cyaR1, affecting regulation of adenylate cyclase expression or activity is described. It was obtained as a thermoresistant revertant of a strain harboring a thermosensitive transcription termination factor, rho (rho-15). This mutant failed to synthesize adenosine 3',5'-phosphate and exhibited a carbohydrate-...

journal_title：Journal of bacteriology

authors： Guidi-Rontani C,Danchin A,Ullmann A

更新日期：1981-12-01 00:00:00

abstract：:It previously has been proposed that the Escherichia coli SecB protein promotes the export of the maltose-binding protein (MBP) from the cytoplasm by preventing the folding of the precursor MBP (preMBP) into a translocation-incompetent conformation. The export of wild-type MBP is only partially blocked in SecB- cells....

journal_title：Journal of bacteriology

authors： Collier DN,Bassford PJ Jr

更新日期：1989-09-01 00:00:00

abstract：:In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...

journal_title：Journal of bacteriology

authors： Frías JE,Flores E

更新日期：2010-06-01 00:00:00

abstract：:ICE6013 represents one of two families of integrative conjugative elements (ICEs) identified in the pan-genome of the human and animal pathogen Staphylococcus aureus Here we investigated the excision and conjugation functions of ICE6013 and further characterized the diversity of this element. ICE6013 excision was not ...

journal_title：Journal of bacteriology

authors： Sansevere EA,Luo X,Park JY,Yoon S,Seo KS,Robinson DA

更新日期：2017-03-28 00:00:00

abstract：:During Bacillus subtilis sporulation, the transient engulfment defect of spoIIB strains is enhanced by spoVG null mutations and suppressed by spoVS null mutations. These mutations have opposite effects on expression of the motility regulon, as the spoVG mutation reduces and the spoVS mutation increases sigmaD-directed...

journal_title：Journal of bacteriology

authors： Perez AR,Abanes-De Mello A,Pogliano K

更新日期：2006-02-01 00:00:00

abstract：:We have previously used the bacteriophage transposon Mu d1 (which encodes the lacZY structural genes but without their promoter) to construct strains that have lacZY fused to phosphate-regulated promoters in Escherichia coli K-12. Among 18 identified phosphate-starvation-inducible (psi) genes, three (the phoA and two ...

journal_title：Journal of bacteriology

authors： Wanner BL,Wieder S,McSharry R

更新日期：1981-04-01 00:00:00

abstract：:No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths ...

journal_title：Journal of bacteriology

authors： Tseng YH,Choy KT,Hung CH,Lin NT,Liu JY,Lou CH,Yang BY,Wen FS,Weng SF,Wu JR

更新日期：1999-01-01 00:00:00

abstract：:The poly-beta-hydroxybutyrate (PHB) biosynthetic pathway from Alcaligenes eutrophus H16 has been cloned and expressed in Escherichia coli. Initially, an A. eutrophus H16 genomic library was constructed by using cosmid pVK102, and cosmid clones that encoded the PHB biosynthetic pathway were sought by assaying for the f...

journal_title：Journal of bacteriology

authors： Slater SC,Voige WH,Dennis DE

更新日期：1988-10-01 00:00:00

abstract：:The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

journal_title：Journal of bacteriology

authors： Johnson GR,Jain RK,Spain JC

更新日期：2002-08-01 00:00:00

abstract：:Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...

journal_title：Journal of bacteriology

authors： Satta G,Fontana R,Canepari P,Botta G

更新日期：1979-02-01 00:00:00

abstract：:We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity ...

journal_title：Journal of bacteriology

authors： Delany I,Pacheco AB,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-08-01 00:00:00

abstract：:To relate the roles of Escherichia coli SSB in recombination in vivo and in vitro, we have studied the mutant proteins SSB-1 and SSB-113, the variant SSBc produced by chymotryptic cleavage, the partially homologous variant F SSB (encoded by the E. coli sex factor), and the protein encoded by gene 32 of bacteriophage T...

journal_title：Journal of bacteriology

authors： Egner C,Azhderian E,Tsang SS,Radding CM,Chase JW

更新日期：1987-08-01 00:00:00

abstract：:The Shiga toxin operon (stx) is composed of two genes for the A and B subunits, which are transcribed from a promoter 5' to the stxA gene. The 1A:5B subunit stoichiometry of the holotoxin suggests that the stxA and stxB genes are differentially regulated. In a previous study, we demonstrated the existence of a second ...

journal_title：Journal of bacteriology

authors： Habib NF,Jackson MP

更新日期：1993-02-01 00:00:00

abstract：:A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...

journal_title：Journal of bacteriology

authors： Beaumont FC,Kang HY,Brickman TJ,Armstrong SK

更新日期：1998-02-01 00:00:00