Detergent-resistant Streptococcus faecium derivatives that display conditional penicillin lysis.

abstract：:An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

journal_title：Journal of bacteriology

authors： Gardner RM,Doerner KC,White BA

更新日期：1987-10-01 00:00:00

abstract：:An incompetent mutant (4S Com(-)) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture me...

journal_title：Journal of bacteriology

authors： Streips UN,Welker NE

更新日期：1971-06-01 00:00:00

abstract：:The mechanism by which iron uptake is facilitated by the siderophore rhodotorulic acid (RA) in the yeast Rhodotorula pilimanae was investigated with radioactively labeled Fe and RA and kinetically inert, chromic-substituted RA complexes. The weight of the evidence supports a model in which RA mediates iron transport t...

journal_title：Journal of bacteriology

authors： Carrano CJ,Raymond KN

更新日期：1978-10-01 00:00:00

abstract：:Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

journal_title：Journal of bacteriology

authors： Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

更新日期：1995-11-01 00:00:00

abstract：:Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguano...

journal_title：Journal of bacteriology

authors： Best AN

更新日期：1978-01-01 00:00:00

abstract：:Salmonella enterica can utilize fructose-asparagine (F-Asn) as a source of carbon and nitrogen. This capability has been attributed to five genes in the fra locus. Previously, we determined that mutations in fraB (deglycase), fraD (kinase), or fraA (transporter) eliminated the ability of Salmonella to grow on F-Asn, w...

journal_title：Journal of bacteriology

authors： Sabag-Daigle A,Sengupta A,Blunk HM,Biswas PK,Cron MC,Bogard AJ,Behrman EJ,Gopalan V,Ahmer BMM

更新日期：2017-10-17 00:00:00

abstract：:EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal S...

journal_title：Journal of bacteriology

authors： Marchès O,Batchelor M,Shaw RK,Patel A,Cummings N,Nagai T,Sasakawa C,Carlsson SR,Lundmark R,Cougoule C,Caron E,Knutton S,Connerton I,Frankel G

更新日期：2006-04-01 00:00:00

abstract：:One step in de novo pyridoxine (vitamin B6) and pyridoxal 5'-phosphate biosynthesis was predicted to be an oxidation catalyzed by an unidentified D-erythrose-4-phosphate dehydrogenase (E4PDH). To help identify this E4PDH, we purified the Escherichia coli K-12 gapA- and gapB-encoded dehydrogenases to homogeneity and te...

journal_title：Journal of bacteriology

authors： Zhao G,Pease AJ,Bharani N,Winkler ME

更新日期：1995-05-01 00:00:00

abstract：:The uptake of iron into Pseudomonas aeruginosa is mediated by two major siderophores produced by the bacterium, pyoverdine and pyochelin. The bacterium is also able of utilize several heterologous siderophores of bacterial or fungal origin. In this work, we have investigated the iron uptake in P. aeruginosa PAO1 by th...

journal_title：Journal of bacteriology

authors： Hannauer M,Barda Y,Mislin GL,Shanzer A,Schalk IJ

更新日期：2010-03-01 00:00:00

abstract：:Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

journal_title：Journal of bacteriology

authors： Golub EI,Low KB

更新日期：1985-04-01 00:00:00

abstract：:Aerosols of Mycoplasma pneumoniae were studied at several relative humidities at a controlled temperature of 27 C. Production of an experimentally reproducible aerosol required preatomization of the organism in its suspending fluid and was dependent on the type of fluid used in atomization as well as on the procedures...

journal_title：Journal of bacteriology

authors： Wright DN,Bailey GD,Hatch MT

更新日期：1968-10-01 00:00:00

abstract：:The whole-genome sequence of Mycobacterium bolletii M24, isolated from the bronchoalveolar lavage fluid of a Malaysian patient, is reported here. The circular chromosome of 5,507,730 bp helped to clarify the taxonomic position of this organism within the M. abscessus complex and revealed the presence of proteins poten...

journal_title：Journal of bacteriology

authors： Wong YL,Choo SW,Tan JL,Ong CS,Ng KP,Ngeow YF

更新日期：2012-08-01 00:00:00

abstract：:Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component o...

journal_title：Journal of bacteriology

authors： Day LE

更新日期：1966-07-01 00:00:00

abstract：:Previously, we characterized a pathway necessary for the processing of NAD+ and for uptake of nicotinamide riboside (NR) in Haemophilus influenzae. Here we report on the role of NadR, which is essential for NAD+ utilization in this organism. Different NadR variants with a deleted ribonucleotide kinase domain or with a...

journal_title：Journal of bacteriology

authors： Merdanovic M,Sauer E,Reidl J

更新日期：2005-07-01 00:00:00

abstract：:Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and her...

journal_title：Journal of bacteriology

authors： Gardner SP,Olson JW

更新日期：2018-10-10 00:00:00

abstract：:Small nuclear ribonucleoprotein (snRNP) particles play an important role in the processing of pre-mRNA. snRNPs have been identified immunologically in a variety of cells, but none have ever been observed in prokaryotic systems. This report provides the first evidence for the presence of snRNP-like components in two ty...

journal_title：Journal of bacteriology

authors： Kovacs SA,O'Neil J,Watcharapijarn J,Moe-Kirvan C,Vijay S,Silva V

更新日期：1993-04-01 00:00:00

abstract：:Staphylococcus lugdunensis is the only coagulase-negative Staphylococcus species with a locus encoding iron-regulated surface determinant (Isd) proteins. In Staphylococcus aureus, the Isd proteins capture heme from hemoglobin and transfer it across the wall to a membrane-bound transporter, which delivers it into the c...

journal_title：Journal of bacteriology

authors： Zapotoczna M,Heilbronner S,Speziale P,Foster TJ

更新日期：2012-12-01 00:00:00

abstract：:D-Ribose isomerase was purified and crystallized from cells of Mycobacterium smegmatis grown on either D-ribose or L-rhamnose. Isomerase activity for both of these sugars remained together throughout the purification. The isomerase from L-rhamnose-grown cells had the same chemical and physical properties as the enzyme...

journal_title：Journal of bacteriology

authors： Izumori K,Mitchell M,Elbein AD

更新日期：1976-04-01 00:00:00

abstract：:NBU1 is an integrated Bacteroides element that can he mobilized from Bacteroides donors to Bacteroides recipients. Previous studies have shown that a plasmid carrying the internal mobilization region of NBU1 could be transferred by conjugation from Bacteroides thetaiotaomicron to Escherichia coli. In this report, we s...

journal_title：Journal of bacteriology

authors： Shoemaker NB,Wang GR,Salyers AA

更新日期：1996-06-01 00:00:00

abstract：:Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. The enzyme has been p...

journal_title：Journal of bacteriology

authors： Brush GS,Bhatnagar SK,Bessman MJ

更新日期：1990-06-01 00:00:00

abstract：:A fusion between lacZ and ftsZ of Escherichia coli was constructed to obtain a beta-galactosidase-FtsZ fusion protein. This fusion protein was used to raise antibodies against cell division protein FtsZ. Six monoclonal antibodies were obtained, and they reacted with FtsZ from cytoplasm and membrane fractions. The epit...

journal_title：Journal of bacteriology

authors： Voskuil JL,Westerbeek CA,Wu C,Kolk AH,Nanninga N

更新日期：1994-04-01 00:00:00

abstract：:SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids with...

journal_title：Journal of bacteriology

authors： Runti G,Lopez Ruiz Mdel C,Stoilova T,Hussain R,Jennions M,Choudhury HG,Benincasa M,Gennaro R,Beis K,Scocchi M

更新日期：2013-12-01 00:00:00

abstract：:The transport system for glycylglycine in Escherichia coli behaves like a shock-sensitive transport system. The initial rate of transport is reduced 85% by subjecting whole cells to osmotic shock, and glycylglycine is not transported by membrane vesicles. The energetics of transport was studied with strain ML 308-225 ...

journal_title：Journal of bacteriology

authors： Cowell JL

更新日期：1974-10-01 00:00:00

abstract：:Graber, C. D. (Baylor University College of Medicine, Houston, Tex.), R. M. O'Neal, and E. R. Rabin. Effect of high fat diets on intestinal microflora and serum cholesterol in rats. J. Bacteriol. 89:47-51. 1965.-Differential bacterial counts of feces and total plasma cholesterol determinations were performed on 60 Wis...

journal_title：Journal of bacteriology

authors： GRABER CD,O'NEAL RM,RABIN ER

更新日期：1965-01-01 00:00:00

abstract：:In many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. We report the characterization of a gene coding for an alkane hydroxylase in a Burkholderia cepacia strain isolated from an oil-contaminated site. The protein encoded showed similarity to ot...

journal_title：Journal of bacteriology

authors： Marín MM,Smits TH,van Beilen JB,Rojo F

更新日期：2001-07-01 00:00:00

abstract：:We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...

journal_title：Journal of bacteriology

authors： Batchelor RA,Haraguchi GE,Hull RA,Hull SI

更新日期：1991-09-01 00:00:00

abstract：:Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...

journal_title：Journal of bacteriology

authors： Hsu CC,Chen CW

更新日期：2010-01-01 00:00:00

abstract：:Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. a...

journal_title：Journal of bacteriology

authors： Jensen RA,Twarog R

更新日期：1972-09-01 00:00:00

abstract：:Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...

journal_title：Journal of bacteriology

authors： Kaneshiro T

更新日期：1968-06-01 00:00:00

abstract：:To circumvent the permeability barrier of its outer membrane, Pseudomonas aeruginosa has evolved a series of specific porins. These channels have binding sites for related classes of molecules that facilitate uptake under nutrient-limited conditions. Here, we report on the identification of a 19-member family of porin...

journal_title：Journal of bacteriology

authors： Tamber S,Ochs MM,Hancock RE

更新日期：2006-01-01 00:00:00