Abstract:
:Welker, N. E. (Western Reserve University, Cleveland, Ohio and University of Illinois, Urbana), and L. Leon Campbell. De novo synthesis of alpha-amylase by Bacillus stearothermophilus. J. Bacteriol. 86:1202-1210. 1963.-The pH optimum for the synthesis of alpha-amylase by washed-cell suspensions was 6.7. alpha-Amylase synthesis began soon after the addition of the inducer (maltose, methyl-beta-d-maltoside, or phenyl-alpha-d-glucoside, at 10(-3)m), proceeded at a linear rate for 60 min, and then leveled off. Cell suspensions without inducer produced small amounts of alpha-amylase. The addition of glucose (2 x 10(-3)m), sucrose (10(-3)m), or glycerol (4 x 10(-3)m) to washed-cell suspensions failed to stimulate the production of alpha-amylase. Nitrogen starvation of washed cells for 60 min with fructose as a carbon source or by induction with pure maltose showed that the ability to produce alpha-amylase was lost. Examination of the amino acid pool at this time showed a general depletion of amino acids and the complete disappearance of tyrosine, phenyl-alanine, proline, and valine. Replenishment of the amino acid pool with casein hydrolysate (0.5%) restored the ability of the cells to produce alpha-amylase. Chloramphenicol and 8-azaguanine were shown to inhibit alpha-amylase synthesis. Inhibition was observed immediately upon the addition of chloramphenicol to cell suspensions preinduced for varying periods of time. Actinomycin D and mitomycin C also inhibited alpha-amylase synthesis when added to induced washed-cell suspensions. The amino acid analogues, norvaline, norleucine, and ethionine, inhibited alpha-amylase formation by 72, 53, and 38%, respectively. p-Fluorophenylalanine inhibited the synthesis of active alpha-amylase by 92% and the incorporation of proline-C(14) into alpha-amylase and cellular proteins by 95 and 74%, respectively.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
WELKER NE,CAMPBELL LLdoi
10.1128/JB.86.6.1202-1210.1963keywords:
subject
Has Abstractpub_date
1963-12-01 00:00:00pages
1202-10eissn
0021-9193issn
1098-5530journal_volume
86pub_type
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