Secretion of nuclease across the outer membrane of Serratia marcescens and its energy requirements.

Abstract:

:Extracellular secretion of Serratia marcescens nuclease occurs as a two-step process via a periplasmic intermediate. Unlike other extracellular proteins secreted by gram-negative bacteria by the general secretory pathway, nuclease accumulates in the periplasm in its active form for an unusually long time before its export into the growth medium. The energy requirements for extracellular secretion of nuclease from the periplasm were investigated. Our results suggest that the second step of secretion across the outer membrane is dependent upon the external pH; acidic pH effectively but reversibly blocks extracellular secretion. However, electrochemical proton gradient, and possibly ATP hydrolysis, are not required for this step. We suggest that nuclease uses a novel mechanism for the second step of secretion in S. marcescens.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Suh Y,Benedik MJ

doi

10.1128/jb.179.3.677-683.1997

subject

Has Abstract

pub_date

1997-02-01 00:00:00

pages

677-83

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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