Abstract:
:The Put3p and Gal4p transcriptional activators are members of a distinct class of fungal regulators called the Cys(6) Zn(II)(2) binuclear cluster family. This family includes over 50 different Saccharomyces cerevisiae proteins that share a similar domain organization. Gal4p activates the genes of the galactose utilization pathway permitting the use of galactose as the sole source of carbon and energy. Put3p controls the expression of the proline utilization pathway that allows yeast cells to grow on proline as the sole nitrogen source. We report that Gal4p can activate the PUT structural genes in a strain lacking Put3p. We also show that the activation of PUT2 by Gal4p depends on the presence of the inducer galactose and the Put3p binding site and that activation increases with increased dosage of Gal4p. Put3p cannot activate the GAL genes in the absence of Gal4p. Our in vivo results confirm previously published in vitro data showing that Gal4p is more promiscuous than Put3p in its DNA binding ability. The results also suggest that under appropriate circumstances, Gal4p may be able to function in place of a related family member to activate expression.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
D'Alessio M,Brandriss MCdoi
10.1128/jb.182.13.3748-3753.2000keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
3748-53issue
13eissn
0021-9193issn
1098-5530journal_volume
182pub_type
杂志文章abstract::Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1315-1320.1987
更新日期:1987-03-01 00:00:00
abstract::A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2720-2723.1992
更新日期:1992-04-01 00:00:00
abstract::Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01489-08
更新日期:2009-04-01 00:00:00
abstract::Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.620-624.1979
更新日期:1979-08-01 00:00:00
abstract::Over most of the range of salt concentrations in which the moderately halophilic bacterium Vibrio costicola could grow, the sum of the cell-associated Na+ + K+ ions was at least as high as in the external medium. This is in contrast to other moderate halophiles, which have substantially lower internal than external sa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.2.698-703.1977
更新日期:1977-05-01 00:00:00
abstract::Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1700-1704.1964
更新日期:1964-12-01 00:00:00
abstract::The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. Muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.139-144.2001
更新日期:2001-01-01 00:00:00
abstract::One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase-like open reading frames (ORFs) were found besides one ORF having the typical alpha-like DNA poly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2232-2236.1998
更新日期:1998-04-01 00:00:00
abstract::The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were identified and characterized. In addition to the known cellulose (cel) and acidic exopolysaccharide (EPS) (pss) genes, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01694-07
更新日期:2008-07-01 00:00:00
abstract::It was shown previously that the disruption of the ahrC gene encoding a predicted ArgR family transcription factor results in a severe defect in biofilm formation in vitro, as well as a significant attenuation of virulence of Enterococcus faecalis strain OG1RF in multiple experimental infection models. Using transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00269-18
更新日期:2018-08-24 00:00:00
abstract::Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.5.1977-1985.1966
更新日期:1966-05-01 00:00:00
abstract::Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.719-727.1976
更新日期:1976-02-01 00:00:00
abstract::The highly conserved DnaK chaperones consist of an N-terminal ATPase domain, a central substrate-binding domain, and a C-terminal domain whose function is not known. Since Bacillus subtilis dnaK was not able to complement an Escherichia coli dnaK null mutant, we performed domain element swap experiments to identify th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.6.1971-1974.1999
更新日期:1999-03-01 00:00:00
abstract::Under anaerobic conditions, Klebsiella pneumoniae reduced nitrite (NO2-), yielding nitrous oxide (N2O) and ammonium ions (NH4+) as products. Nitrous oxide formation accounted for about 5% of the total NO2- reduced, and NH4+ production accounted for the remainder. Glucose and pyruvate were the electron donors for NO2- ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.454-458.1983
更新日期:1983-08-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) O157:H7 responds to the host-produced epinephrine and norepinephrine, and bacterially produced autoinducer 3 (AI-3), through two-component systems. Further integration of multiple regulatory signaling networks, involving regulators such as the LysR-type transcriptional regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00382-10
更新日期:2010-07-01 00:00:00
abstract::A 40-kb region of DNA from Sorangium cellulosum So ce26, which contains polyketide synthase (PKS) genes for synthesis of the antifungal macrolide antibiotic soraphen A, was cloned. These genes were detected by homology to Streptomyces violaceoruber genes encoding components of granaticin PKS, thus extending this power...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.13.3673-3679.1995
更新日期:1995-07-01 00:00:00
abstract::Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00718-18
更新日期:2019-05-08 00:00:00
abstract::Bacteriocin 41 (Bac41) is produced from clinical isolates of Enterococcus faecalis and consists of two extracellular proteins, BacL1 and BacA. We previously reported that BacL1 protein (595 amino acids, 64.5 kDa) is a bacteriolytic peptidoglycan D-isoglutamyl-L-lysine endopeptidase that induces cell lysis of E. faecal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02203-14
更新日期:2015-01-01 00:00:00
abstract::An extracellular, 700,000-Mr multiprotein complex that catalyzed the hydrolysis of crystalline cellulose (Avicel) was isolated from cultures of Clostridium sp. strain C7, a mesophile from freshwater sediment. In addition to cellulose (Avicel, ball-milled filter paper), the multiprotein complex hydrolyzed carboxymethyl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.8.4231-4237.1990
更新日期:1990-08-01 00:00:00
abstract::We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.7.2711-2714.2006
更新日期:2006-04-01 00:00:00
abstract::Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01412-08
更新日期:2009-04-01 00:00:00
abstract::The inner or cytoplasmic membrane fraction of the cell envelope of Escherichia coli was isolated by isopycnic centrifugation on sucrose gradients. The membrane proteins were analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels (8.5%), and up to 56 bands were resolved. Different preparations gave v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.947-953.1974
更新日期:1974-03-01 00:00:00
abstract::Intact yeast cells treated with alkali cations took up plasmid DNA. Li+, Cs+, Rb+, K+, and Na+ were effective in inducing competence. Conditions for the transformation of Saccharomyces cerevisiae D13-1A with plasmid YRp7 were studied in detail with CsCl. The optimum incubation time was 1 h, and the optimum cell concen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.163-168.1983
更新日期:1983-01-01 00:00:00
abstract::The concentrations of all metabolites studied, except fructose 1,6-bisphosphate from wild-type Phycomyces blakesleeanus, were light dependent. This photoregulation appears to be independent of the mad gene product(s) and also independent of carotene biosynthesis regulation. However, the photoregulation of glyceraldehy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.904-907.1987
更新日期:1987-02-01 00:00:00
abstract::Heating at 55 C causes the intracellular degradation of ribosomes of Staphylococcus aureus MF-31. The 30S subunit of heated cells appears to be selectively attacked. Analysis of ribonucleic acid (RNA) showed that 16S RNA was destroyed and that the secondary structure of 23S RNA was altered. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.3.833-835.1970
更新日期:1970-09-01 00:00:00
abstract::Mated cultures of Blakeslea trispora, grown in a potato extract-glucose-thiamine medium, produced 10 to 15 times more beta-carotene than either unmated culture. Mated, but not unmated, cultures produced a family of compounds (beta factor) which stimulated carotenogenesis in unmated cultures. In fact, carotenogenesis w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.426-432.1968
更新日期:1968-02-01 00:00:00
abstract::The virulence of Staphylococcus aureus is controlled by the accessory gene regulator (agr) system, including an extracellular inducer encoded by agrD. Variable agr PCR restriction fragment length polymorphism (RFLP) patterns of unique S. aureus strains (n = 192) were determined for a region comprising agrD and parts o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.20.5721-5729.2000
更新日期:2000-10-01 00:00:00
abstract::The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein, which had been isolat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3730-3737.1990
更新日期:1990-07-01 00:00:00
abstract::Fifteen low-temperature conditional division mutants of Escherichia coli K-12 was isolated. They grew normally at 39 degrees C but formed filaments at 30 degrees C. All exhibited a coordinated burst of cell division when the filaments were shifted to the permissive temperature (39 degrees C). None of the various agent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.256-264.1978
更新日期:1978-01-01 00:00:00
abstract::The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.1016-1024.1967
更新日期:1967-10-01 00:00:00