Proteins of the inner membrane of Escherichia coli: identification of succinate dehydrogenase by polyacrylamide gel electrophoresis with sdh amber mutants.

abstract：:The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...

journal_title：Journal of bacteriology

authors： Strazdis JR,MacKay VL

更新日期：1982-09-01 00:00:00

abstract：:Enterococcus faecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2, NH3, and the end products ornithine and putrescine, respectively. In the arginine deiminase pathway, uptake of arginine ...

journal_title：Journal of bacteriology

authors： Driessen AJ,Smid EJ,Konings WN

更新日期：1988-10-01 00:00:00

abstract：:A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

journal_title：Journal of bacteriology

authors： Calsou P,Salles B

更新日期：1985-06-01 00:00:00

abstract：:In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...

journal_title：Journal of bacteriology

authors： Kuty GF,Xu M,Struck DK,Summer EJ,Young R

更新日期：2010-11-01 00:00:00

abstract：:Escherichia coli mutant N4316 is temperature sensitive and exhibits temperature-dependent suppression. These phenotypes are due to separate genes, as shown by reversion and mapping studies. The suppressor mutation was mapped and lies near argF. ...

journal_title：Journal of bacteriology

authors： Herrington MB,Ganoza MC

更新日期：1977-02-01 00:00:00

abstract：:Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, o...

journal_title：Journal of bacteriology

authors： Johnson WC,Tipper DJ

更新日期：1981-06-01 00:00:00

abstract：:Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

journal_title：Journal of bacteriology

authors： Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

更新日期：2008-03-01 00:00:00

abstract：:Bradyrhizobium diazoefficiens is a soil alphaproteobacterium that possesses two evolutionarily distinct flagellar systems, a constitutive subpolar flagellum and inducible lateral flagella that, depending on the carbon source, may be expressed simultaneously in liquid medium and used interactively for swimming. In each...

journal_title：Journal of bacteriology

authors： Mengucci F,Dardis C,Mongiardini EJ,Althabegoiti MJ,Partridge JD,Kojima S,Homma M,Quelas JI,Lodeiro AR

更新日期：2020-02-11 00:00:00

abstract：:Teichoic acid-like material extracted by cold trichloroacetic acid from lyophilized whole cells of streptococci from groups A,D,E,O, and T was shown to give a positive precipitin reaction with group antisera. Similar material from cells of groups B,C,F,G,H,K,L,M,N,P,Q,R, and S did not give a positive reaction with gro...

journal_title：Journal of bacteriology

authors： Matsuno T,Slade HD

更新日期：1970-06-01 00:00:00

abstract：:We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...

journal_title：Journal of bacteriology

authors： Eggers CH,Kimmel BJ,Bono JL,Elias AF,Rosa P,Samuels DS

更新日期：2001-08-01 00:00:00

abstract：:We isolated several new mutator mutations of the Escherichia coli replicative polymerase dnaE subunit alpha and used them and a previously reported dnaE mutation to study spontaneous frameshift and base substitution mutations. Two of these dnaE strains produce many more mutants when grown on rich (Luria-Bertani) than ...

journal_title：Journal of bacteriology

authors： Strauss BS,Roberts R,Francis L,Pouryazdanparast P

更新日期：2000-12-01 00:00:00

abstract：:CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...

journal_title：Journal of bacteriology

authors： Johnson VG,Nicholls PJ

更新日期：1994-08-01 00:00:00

abstract：:Group A streptococcus (GAS) produces millions of infections worldwide, including mild mucosal infections, postinfection sequelae, and life-threatening invasive diseases. During infection, GAS readily acquires nutritional iron from host heme and hemoproteins. Here, we identified a new heme importer, named SiaFGH, and i...

journal_title：Journal of bacteriology

authors： Chatterjee N,Cook LCC,Lyles KV,Nguyen HAT,Devlin DJ,Thomas LS,Eichenbaum Z

更新日期：2020-06-25 00:00:00

abstract：:Three polynucleotide phosphorylase mutations, isolated in heavily mutagenized Escherichia coli strains Q7, Q13, and Q27, were characterized after their transfer by P1 transduction to nearly isogenic strains which lack ribonuclease I. Each strain has a different altered form of polynucleotide phosphorylase. One enzyme ...

journal_title：Journal of bacteriology

authors： Reiner AM

更新日期：1969-03-01 00:00:00

abstract：:Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...

journal_title：Journal of bacteriology

authors： Flannagan SE,Clewell DB

更新日期：1991-11-01 00:00:00

abstract：:Bdellovibrio bacteriovorus is an obligate predatory bacterium that invades and kills a broad range of Gram-negative prey cells, including human pathogens. Its potential therapeutic application has been the subject of increased research interest in recent years. However, an improved understanding of the fundamental mol...

journal_title：Journal of bacteriology

authors： Bukowska-Faniband E,Andersson T,Lood R

更新日期：2020-08-25 00:00:00

abstract：:The human pathogen Eikenella corrodens synthesizes type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonie...

journal_title：Journal of bacteriology

authors： Villar MT,Helber JT,Hood B,Schaefer MR,Hirschberg RL

更新日期：1999-07-01 00:00:00

abstract：:The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in ana...

journal_title：Journal of bacteriology

authors： Baruah A,Lindsey B,Zhu Y,Nakano MM

更新日期：2004-03-01 00:00:00

abstract：:Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

journal_title：Journal of bacteriology

authors： Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

更新日期：1998-04-01 00:00:00

abstract：:Erythrobacter litoralis has been known as a bacteriochlorophyll a-containing, aerobic, anoxygenic, phototrophic bacterium. Here we announce the complete genome sequence of E. litoralis HTCC2594, which is devoid of phototrophic potential. E. litoralis HTCC2594, isolated by dilution-to-extinction culturing from seawater...

journal_title：Journal of bacteriology

authors： Oh HM,Giovannoni SJ,Ferriera S,Johnson J,Cho JC

更新日期：2009-04-01 00:00:00

abstract：:While the protein complex responsible for controlling the direction (clockwise [CW] or counterclockwise [CCW]) of flagellar rotation has been fairly well studied in Escherichia coli and Salmonella, less is known about the switch complex in Bacillus subtilis or other Gram-positive species. Two component proteins (FliG ...

journal_title：Journal of bacteriology

authors： Ward E,Kim EA,Panushka J,Botelho T,Meyer T,Kearns DB,Ordal G,Blair DF

更新日期：2019-03-26 00:00:00

abstract：:The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...

journal_title：Journal of bacteriology

authors： Wu CY,Blaszczak LC,Smith MC,Skatrud PL

更新日期：1994-03-01 00:00:00

abstract：:The ferric enterobactin (FeEnt) receptor CfrA is present in the majority of Campylobacter jejuni isolates and is responsible for high-affinity iron acquisition. Our recent work and that of others strongly suggested the existence of another FeEnt uptake system in Campylobacter. Here we have identified and characterized...

journal_title：Journal of bacteriology

authors： Xu F,Zeng X,Haigh RD,Ketley JM,Lin J

更新日期：2010-09-01 00:00:00

abstract：:In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...

journal_title：Journal of bacteriology

authors： Frías JE,Flores E

更新日期：2010-06-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...

journal_title：Journal of bacteriology

authors： Young-Robbins SS,Risser DD,Moran JR,Haselkorn R,Callahan SM

更新日期：2010-09-01 00:00:00

abstract：:The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...

journal_title：Journal of bacteriology

authors： Ofek I,Simpson WA,Beachey EH

更新日期：1982-02-01 00:00:00

abstract：:Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919-923. 1962.-Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli wer...

journal_title：Journal of bacteriology

authors： SHAPIRA J,LOWDEN L,HALE R

更新日期：1962-04-01 00:00:00

abstract：:The O-repeating unit of the Escherichia coli O7-specific lipopolysaccharide is made of galactose, mannose, rhamnose, 4-acetamido-4,6-dideoxyglucose, and N-acetyglucosamine. We have recently characterized the genes involved in the biosynthesis of the sugar precursor GDP-mannose occurring in the E. coli O7:K1 strain VW1...

journal_title：Journal of bacteriology

authors： Marolda CL,Valvano MA

更新日期：1995-10-01 00:00:00

abstract：:An extracellular, 700,000-Mr multiprotein complex that catalyzed the hydrolysis of crystalline cellulose (Avicel) was isolated from cultures of Clostridium sp. strain C7, a mesophile from freshwater sediment. In addition to cellulose (Avicel, ball-milled filter paper), the multiprotein complex hydrolyzed carboxymethyl...

journal_title：Journal of bacteriology

authors： Cavedon K,Leschine SB,Canale-Parola E

更新日期：1990-08-01 00:00:00

abstract：:Wallace, W. R. (Louisiana State University, Baton Rouge), and G. T. Dimopoullos. Catalase activity in Anaplasma marginale. J. Bacteriol. 91:309-311. 1965.-Extracts of erythrocytes infected with Anaplasma marginale were found to contain more catalase activity than normal erythrocytic preparations. The increase in catal...

journal_title：Journal of bacteriology

authors： WALLACE WR,DIMOPOULLOS GT

更新日期：1965-08-01 00:00:00