Abstract:
:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucleotides -305, -614, and -645 relative to the translational start site (-305, -614, and -645 tsps). Transcriptional reporter fusions were used to show that transcription from the -305 tsp was induced in all cells of filaments in response to nitrogen deprivation, required hetR for induction, and increased in a patA mutant. Transcription from -614/-645 tsp reporter fusions was spatially regulated and occurred primarily in cells that would become heterocysts. Complementation of a patA mutant strain by alleles encoding substitutions in, or deletion of, the putative phosphoacceptor C-terminal domain indicates that the PATAN domain can function independently of the C-terminal domain of PatA. Localization of a ring of PatA-GFP at sites of cell division, as well as the formation of enlarged cells with altered cell morphology when patA was overexpressed, suggests that PatA may participate in cell division.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Young-Robbins SS,Risser DD,Moran JR,Haselkorn R,Callahan SMdoi
10.1128/JB.00577-10subject
Has Abstractpub_date
2010-09-01 00:00:00pages
4732-40issue
18eissn
0021-9193issn
1098-5530pii
JB.00577-10journal_volume
192pub_type
杂志文章abstract::Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier s...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1978-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-12-01 00:00:00
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更新日期:2005-10-01 00:00:00
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更新日期:1985-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-01-01 00:00:00
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更新日期:1971-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5661-5671.2004
更新日期:2004-09-01 00:00:00