Regulation of a phage endolysin by disulfide caging.

abstract：:Escherichia coli suffered 95 to 100% lethality when exposed to 1.0 mM paraquat for 30 min at 37 degrees C in aerobic nutrient broth medium but did not lose viability when the exposure was done in Vogel Bonner or tryptic soy yeast extract medium. Paraquat was, however, bacteriostatic in all of these media. Salts, added...

journal_title：Journal of bacteriology

authors： Kitzler J,Fridovich I

更新日期：1986-07-01 00:00:00

abstract：:A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synth...

journal_title：Journal of bacteriology

authors： Guardiola J,De Felice M,Iaccarino M

更新日期：1974-10-01 00:00:00

abstract：:A characteristic of N2-fixing cyanobacteria in symbiotic associations appears to be release of N2-derived NH4+. The specific activity of the primary ammonium-assimilating enzyme, glutamine synthetase (GS), was found to be three- to fourfold lower in Nostoc sp. strain 7801 grown in symbiotic association with the bryoph...

journal_title：Journal of bacteriology

authors： Joseph CM,Meeks JC

更新日期：1987-06-01 00:00:00

abstract：:Mild alkaline extraction of native poly-beta-hydroxybutyrate (PHB) granules results in the solubilization of a protein fraction. Both the solubilized protein fraction and the extracted granules are essentially devoid of PHB synthetase activity unless recombined. The protein fraction has been separated by chromatograph...

journal_title：Journal of bacteriology

authors： Griebel RJ,Merrick JM

更新日期：1971-11-01 00:00:00

abstract：:The maltose regulon consists of three operons controlled by a positive regulatory gene, malT. Deletions of the gene crp were introduced into strains which carried a malT-lacZ hybrid gene. From the observed reduction in beta-galactosidase activity it was concluded that the expression of malT-lacZ, and therefore of malT...

journal_title：Journal of bacteriology

authors： Chapon C

更新日期：1982-05-01 00:00:00

abstract：:Cholera, a severe diarrheal disease, is caused by ingestion of the gram-negative bacterium Vibrio cholerae. Expression of V. cholerae virulence factors is highly regulated at the transcriptional and posttranscriptional levels by a complex network of proteins and small noncoding RNAs. The direct activator of transcript...

journal_title：Journal of bacteriology

authors： Bellair M,Withey JH

更新日期：2008-12-01 00:00:00

abstract：:Enzymes of the ATP-independent Deg serine endopeptidase family are very flexible with regard to their substrate specificity. Some family members cleave only one substrate, while others act as general proteases on unfolded substrates. The proteolytic activity of Deg proteases is regulated by PDZ protein interaction dom...

journal_title：Journal of bacteriology

authors： Huesgen PF,Scholz P,Adamska I

更新日期：2007-09-01 00:00:00

abstract：:Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...

journal_title：Journal of bacteriology

authors： Thiele I,Vo TD,Price ND,Palsson BØ

更新日期：2005-08-01 00:00:00

abstract：:Nosocomial infections caused by antibiotic-resistant Klebsiella pneumoniae are emerging as a major health problem worldwide, while community-acquired K. pneumoniae infections present with a range of diverse clinical pictures in different geographic areas. In particular, an invasive form of K. pneumoniae that causes li...

journal_title：Journal of bacteriology

authors： Wu KM,Li LH,Yan JJ,Tsao N,Liao TL,Tsai HC,Fung CP,Chen HJ,Liu YM,Wang JT,Fang CT,Chang SC,Shu HY,Liu TT,Chen YT,Shiau YR,Lauderdale TL,Su IJ,Kirby R,Tsai SF

更新日期：2009-07-01 00:00:00

abstract：:An enzyme which liberates Pi from myo-inositol hexaphosphate (phytic acid) was shown to be present in culture filtrates of Bacillus subtilis. It was purified until it was homogeneous by ultracentrifugation, but it still showed two isozymes on polyacrylamide gel electrophoresis. The enzyme differed from other previousl...

journal_title：Journal of bacteriology

authors： Powar VK,Jagannathan V

更新日期：1982-09-01 00:00:00

abstract：:Intracellular accumulation of nitrate, indicative of the operation of an active nitrate transport system, has been measured in intact cells of the cyanobacterium Anacystis nidulans. The ability of the cells to accumulate nitrate was effectively hindered by either ammonium addition or selective inhibition of CO2 fixati...

journal_title：Journal of bacteriology

authors： Lara C,Romero JM,Guerrero MG

更新日期：1987-09-01 00:00:00

abstract：:Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-defi...

journal_title：Journal of bacteriology

authors： Page CA,Krause DC

更新日期：2013-04-01 00:00:00

abstract：:The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) an...

journal_title：Journal of bacteriology

authors： Narberhaus F,Weiglhofer W,Fischer HM,Hennecke H

更新日期：1996-09-01 00:00:00

abstract：:The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...

journal_title：Journal of bacteriology

authors： Rosta S,Mett H

更新日期：1989-01-01 00:00:00

abstract：:Coxiella burnetii is a Gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. In this study, we characterized a thiol-specific peroxidase of C. burnetii that belongs to the atypica...

journal_title：Journal of bacteriology

authors： Hicks LD,Raghavan R,Battisti JM,Minnick MF

更新日期：2010-04-01 00:00:00

abstract：:Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,00...

journal_title：Journal of bacteriology

authors： Schulman M,Valentino D

更新日期：1976-10-01 00:00:00

abstract：:Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...

journal_title：Journal of bacteriology

authors： Walderich B,Ursinus-Wössner A,van Duin J,Höltje JV

更新日期：1988-11-01 00:00:00

abstract：:The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...

journal_title：Journal of bacteriology

authors： Stibitz S,Weiss AA,Falkow S

更新日期：1988-07-01 00:00:00

abstract：:The Bacillus subtilis anti-TRAP protein regulates the ability of the tryptophan-activated TRAP protein to bind to trp operon leader RNA and promote transcription termination. AT synthesis is regulated both transcriptionally and translationally by uncharged tRNA(Trp). In this study, we examined the roles of AT synthesi...

journal_title：Journal of bacteriology

authors： Cruz-Vera LR,Gong M,Yanofsky C

更新日期：2008-03-01 00:00:00

abstract：:Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many beta-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene ...

journal_title：Journal of bacteriology

authors： Doménech-Sánchez A,Hernández-Allés S,Martínez-Martínez L,Benedí VJ,Albertí S

更新日期：1999-05-01 00:00:00

abstract：:A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...

journal_title：Journal of bacteriology

authors： Zuerner RL,Stanton TB

更新日期：1994-02-01 00:00:00

abstract：:The PmrA/PmrB two-component system encoded by the pmrCAB operon regulates the modification of Salmonella enterica serovar Typhimurium lipopolysaccharide leading to polymyxin B resistance. PmrA and PhoP are the only known activators of pmrCAB. A transposon mutagenesis screen for additional regulators of a pmrC::MudJ fu...

journal_title：Journal of bacteriology

authors： Merighi M,Carroll-Portillo A,Septer AN,Bhatiya A,Gunn JS

更新日期：2006-01-01 00:00:00

abstract：:The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...

journal_title：Journal of bacteriology

authors： Gao T,O'Brian MR

更新日期：2005-08-01 00:00:00

abstract：:Strain 768-28 was isolated from a hot spring in Kamchatka, Russia, and represents a novel member of the Vulcanisaeta genus. The complete genome sequence of this thermoacidophilic anaerobic crenarchaeon reveals genes for protein and carbohydrate-active enzymes, the Embden-Meyerhof and Entner-Doudoroff pathways for gluc...

journal_title：Journal of bacteriology

authors： Gumerov VM,Mardanov AV,Beletsky AV,Prokofeva MI,Bonch-Osmolovskaya EA,Ravin NV,Skryabin KG

更新日期：2011-05-01 00:00:00

abstract：:Rhodocyclales are representative of versatile bacteria that are able to utilize a wide variety of organic compounds for growth, but only a few strains have been isolated in pure culture thus far. Here we present the genome sequence of Methyloversatilis universalis FAM5(T), the first cultivable methylotrophic member of...

journal_title：Journal of bacteriology

authors： Kittichotirat W,Good NM,Hall R,Bringel F,Lajus A,Médigue C,Smalley NE,Beck D,Bumgarner R,Vuilleumier S,Kalyuzhnaya MG

更新日期：2011-09-01 00:00:00

abstract：:Oxolinic acid reduced RNA synthesis rates whether chromosome supercoiling decreased, increased, or remained unchanged. Thus, inhibition of RNA synthesis by oxolinic acid appears to involve factors other than average DNA supercoiling level. Coumermycin A1 caused RNA synthesis rates to increase or decrease roughly in pa...

journal_title：Journal of bacteriology

authors： Manes SH,Pruss GJ,Drlica K

更新日期：1983-07-01 00:00:00

abstract：:ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...

journal_title：Journal of bacteriology

authors： Tanaka S,Lee SO,Hamaoka K,Kato J,Takiguchi N,Nakamura K,Ohtake H,Kuroda A

更新日期：2003-09-01 00:00:00

abstract：:The response of Neurospora to changes in the availibility of exogenous arginine was investigated. Upon addition of arginine to the growth medium, catabolism is initiated within minutes. This occurs prior to expansion of the arginine pool or augmentation of catabolic enzyme levels. (Basal levels are approximately 25% o...

journal_title：Journal of bacteriology

authors： Weiss RL,Davis RH

更新日期：1977-02-01 00:00:00

abstract：:The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth r...

journal_title：Journal of bacteriology

authors： Kubitschek HE,Baldwin WW,Schroeter SJ,Graetzer R

更新日期：1984-04-01 00:00:00

abstract：:Lysyl-transfer ribonucleic acid synthetase (EC 6.1.1.6) was identified as four polypeptide spots after two-dimensional polyacrylamide gel electrophoresis of whole-cell lysates of Escherichia coli. Identification was made by migration with partially purified enzyme preparations, by peptide map patterns, by mutant analy...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bloch PL,Van Bogelen RA,Neidhardt FC

更新日期：1981-04-01 00:00:00