Characterization of polynucleotide phosphorylase mutants of Escherichia coli.

abstract：:We previously clarified that the chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 produces diacetylchitobiose (GlcNAc(2)) as an end product from chitin. Here we sought to identify enzymes in T. kodakaraensis that were involved in the further degradation of GlcNAc(2). Through a search of th...

journal_title：Journal of bacteriology

authors： Tanaka T,Fukui T,Atomi H,Imanaka T

更新日期：2003-09-01 00:00:00

abstract：:The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...

journal_title：Journal of bacteriology

authors： McBeth DL

更新日期：1989-02-01 00:00:00

abstract：:Pantothenate kinase (CoaA) is a key regulator of coenzyme A (CoA) biosynthesis in Escherichia coli, and its activity is controlled by feedback inhibition by CoA and its thioesters. The importance of feedback inhibition in the control of the intracellular CoA levels was tested by constructing three site-directed mutant...

journal_title：Journal of bacteriology

authors： Rock CO,Park HW,Jackowski S

更新日期：2003-06-01 00:00:00

abstract：:We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

journal_title：Journal of bacteriology

authors： Rothstein DM,Magasanik B

更新日期：1980-02-01 00:00:00

abstract：:Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows. ...

journal_title：Journal of bacteriology

authors： Kim KS,Barksdale L

更新日期：1969-06-01 00:00:00

abstract：:The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alp...

journal_title：Journal of bacteriology

authors： Yamaguchi K,Nagata Y,Maruo B

更新日期：1974-08-01 00:00:00

abstract：:The secondary structures of the eubacterial RNase P RNAs are being elucidated by a phylogenetic comparative approach. Sequences of genes encoding RNase P RNA from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. These sequences allow the refinement, to ne...

journal_title：Journal of bacteriology

authors： Brown JW,Haas ES,James BD,Hunt DA,Liu JS,Pace NR

更新日期：1991-06-01 00:00:00

abstract：:Grula, Mary M. (Oklahoma State University, Stillwater) and E. A. Grula. Cell division in a species of Erwinia. IV. Metabolic blocks in pantothenate biosynthesis and their relationship to inhibition of cell division. J. Bacteriol. 83:989-997. 1962.-Four compounds that inhibit cell division in an Erwinia sp., d-serine, ...

journal_title：Journal of bacteriology

authors： GRULA MM,GRULA EA

更新日期：1962-05-01 00:00:00

abstract：:Type 1 fimbriae and flagella have been previously shown to contribute to the virulence of uropathogenic Escherichia coli (UPEC) within the urinary tract. In this study, the relationship between motility and type 1 fimbrial expression was tested for UPEC strain CFT073 by examining the phenotypic effect of fimbrial expr...

journal_title：Journal of bacteriology

authors： Lane MC,Simms AN,Mobley HL

更新日期：2007-08-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:Clostridium perfringens causes gas gangrene and gastrointestinal (GI) diseases in humans. The most common cause of C. perfringens-associated food poisoning is the consumption of C. perfringens vegetative cells followed by sporulation and production of enterotoxin in the gut. Despite the importance of spore formation i...

journal_title：Journal of bacteriology

authors： Ohtani K,Hirakawa H,Paredes-Sabja D,Tashiro K,Kuhara S,Sarker MR,Shimizu T

更新日期：2013-06-01 00:00:00

abstract：:MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...

journal_title：Journal of bacteriology

authors： Kehres DG,Janakiraman A,Slauch JM,Maguire ME

更新日期：2002-06-01 00:00:00

abstract：:Escherichia coli cells expressing the tpm gene encoding the bacterial thiopurine methyltransferase (bTPMT) are shown to methylate selenite and (methyl)selenocysteine into dimethylselenide (DMSe) and dimethyldiselenide (DMDSe). E. coli cells expressing tpm from a gene library cosmid clone (harboring a Pseudomonas syrin...

journal_title：Journal of bacteriology

authors： Ranjard L,Prigent-Combaret C,Nazaret S,Cournoyer B

更新日期：2002-06-01 00:00:00

abstract：:The model archaeon Pyrococcus furiosus grows optimally near 100°C on carbohydrates and peptides. Its genome sequence (NCBI) was determined 12 years ago. A genetically tractable strain, COM1, was very recently reported, and here we describe its genome sequence. Of 1,909,827 bp in size, it is 1,571 bp longer (0.1%) than...

journal_title：Journal of bacteriology

authors： Bridger SL,Lancaster WA,Poole FL 2nd,Schut GJ,Adams MW

更新日期：2012-08-01 00:00:00

abstract：:Aggregative amoebae of Dictyostelium discoideum, D. mucoroides, D. purpureum, and D. rosarium react chemotactically to cyclic AMP (cAMP). We measured the chemotactic activity of 14 cAMP analogs and found that these four species have a similar sensitivity to chemical modifications of cAMP; this suggests that the cAMP r...

journal_title：Journal of bacteriology

authors： Van Haastert PJ,Jastorff B,Pinas JE,Konijn TM

更新日期：1982-01-01 00:00:00

abstract：:A system for high-level expression of heparinase I, heparinase II, heparinase III, chondroitinase AC, and chondroitinase B in Flavobacterium heparinum is described. hepA, along with its regulatory region, as well as hepB, hepC, cslA, and cslB, cloned downstream of the hepA regulatory region, was integrated in the chro...

journal_title：Journal of bacteriology

authors： Blain F,Tkalec AL,Shao Z,Poulin C,Pedneault M,Gu K,Eggimann B,Zimmermann J,Su H

更新日期：2002-06-01 00:00:00

abstract：:Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...

journal_title：Journal of bacteriology

authors： Kang J,Blaser MJ

更新日期：2008-06-01 00:00:00

abstract：:The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...

journal_title：Journal of bacteriology

authors： Samaluru H,SaiSree L,Reddy M

更新日期：2007-11-01 00:00:00

abstract：:Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Iwashima A,Nose Y

更新日期：1972-12-01 00:00:00

abstract：:Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...

journal_title：Journal of bacteriology

authors： Rifkind D,Palmer JD

更新日期：1966-10-01 00:00:00

abstract：:In Escherichia coli, the SecB/SecA branch of the Sec pathway and the twin-arginine translocation (Tat) pathway represent two alternative possibilities for posttranslational translocation of proteins across the cytoplasmic membrane. Maintenance of pathway specificity was analyzed using a model precursor consisting of t...

journal_title：Journal of bacteriology

authors： Blaudeck N,Kreutzenbeck P,Freudl R,Sprenger GA

更新日期：2003-05-01 00:00:00

abstract：:In Streptococcus mutans, competence for genetic transformation and biofilm formation are dependent on the two-component signal transduction system ComDE together with the inducer peptide pheromone competence-stimulating peptide (CSP) (encoded by comC). Here, it is shown that the same system is also required for expres...

journal_title：Journal of bacteriology

authors： van der Ploeg JR

更新日期：2005-06-01 00:00:00

abstract：:The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...

journal_title：Journal of bacteriology

authors： Zhang YX,Shi Y,Zhou M,Petsko GA

更新日期：1994-02-01 00:00:00

abstract：:In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...

journal_title：Journal of bacteriology

authors： Kuty GF,Xu M,Struck DK,Summer EJ,Young R

更新日期：2010-11-01 00:00:00

abstract：:The formation of the five tryptophan biosynthetic enzymes of Neurospora crassa was shown to be derepressed in histidine-starved cells. This histidine-mediated derepression was not due to a lowered intracellular concentration of tryptophan in these cells. Furthermore, histidine-mediated derepression of tryptophan enzym...

journal_title：Journal of bacteriology

authors： Carsiotis M,Jones RF,Lacy AM,Cleary TJ,Fankhauser DB

更新日期：1970-10-01 00:00:00

abstract：:The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo rep...

journal_title：Journal of bacteriology

authors： Wray LV Jr,Reznikoff WS

更新日期：1983-12-01 00:00:00

abstract：:Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the...

journal_title：Journal of bacteriology

authors： Toh E,Kurtz HD Jr,Brun YV

更新日期：2008-11-01 00:00:00

abstract：:The metabolism of mRNA from the lactose (lac) operon of Escherichia coli has been studied in ribonuclease (RNase) III-deficient strains (rnc-105). The induction lag for beta-galactosidase from the first gene was twice as long, and enzyme synthesis was reduced 10-fold in one such mutant compared with its isogenic rnc+ ...

journal_title：Journal of bacteriology

authors： Talkad V,Achord D,Kennell D

更新日期：1978-08-01 00:00:00

abstract：:The heat shock response of the groESL operon of Agrobacterium tumefaciens was studied at the RNA level. The operon was found to be activated under heat shock conditions and transcribed as a polycistronic mRNA that contains the groES and groEL genes. After activation, the polycistronic mRNA appeared to be cleaved betwe...

journal_title：Journal of bacteriology

authors： Segal G,Ron EZ

更新日期：1995-02-01 00:00:00

abstract：:Synthesis of formate dehydrogenase coupled to formate hydrogenlyase activity in Escherichia coli was found to require the product of the fhlA gene. Transcription of fdhF, the gene coding for the 80-kilodalton (kDa) selenopeptide of formate dehydrogenase, was not detected in an fhlA genetic background. Mutations in the...

journal_title：Journal of bacteriology

authors： Sankar P,Lee JH,Shanmugam KT

更新日期：1988-12-01 00:00:00